Hybrid quadrupole-time-of-flight mass spectrometer and chromatography system

 申请者描述(由申请人提供)：安捷伦6550混合式四极杆飞行时间质谱仪(QTOF)与安捷伦1290超高压液相色谱系统(UHPLC)一起申请资金。该设备将设在帕萨罗质量分析实验室(PMSL)，这是加州大学洛杉矶分校塞梅尔研究所的一个合作核心实验室。PMSL目前没有适用于液-质联用(LC/MS)代谢组学或蛋白质组学实验的仪器。这一文书对于支持私营部门和劳工部参与并赢得高度评价的一系列研究项目至关重要。采购所需设备将确保PMSL及其合作实验室在各自领域保持竞争力，并继续在它们正在开展的许多重要和令人振奋的研究项目上取得进展。所要求的仪器将用于的项目包括基础、翻译和临床科学，涉及对包括阿尔茨海默氏症和相关疾病、各种癌症和缺铁性贫血等多种疾病的分子生物标志物筛查的尖端研究。在这些实验中被鉴定和量化的化合物与多种生物现象有关，包括信号转导、细胞通讯、激素稳态、成瘾过程、衰老、免疫反应和炎症。本申请包括23个合作实验室的项目描述：6个被指定为主要项目，其余被指定为次要项目，这一区别的基础是仪器和人员的总体时间投入，所有这些都同样重要和令人兴奋。这些协作预计将占用大约90%的仪器时间。剩余的仪器时间将用于其他合作小组进行短期探索性研究。PMSL拥有维护和支持新设备的基础设施，并有出色的记录支持，促进了当代质谱学的应用，以解决科学和医学中的重要生物学问题。负责这项申请的PPI和PMSL主任、PMSL事实上的副主任Whitelgge教授以及研究助理Joseph Capri和Farbod Fazlollahi将负责新设备的日常操作和维护。福尔教授和惠特勒格教授在有效使用通过NIH SIG机制购买的设备方面享有非凡的声誉。这项应用是加州大学洛杉矶分校、大卫·格芬医学院和塞梅尔研究所努力的关键组成部分，目的是为协作的校园核心实验室配备代谢组学、蛋白质组学和相关领域的尖端研究工具。

 DESCRIPTION (provided by applicant): Funds are requested for an Agilent 6550 hybrid quadrupole-time-of-flight mass spectrometer (QTOF) with an Agilent 1290 ultra-high pressure liquid chromatography system (UHPLC). This equipment will be located in the Pasarow Mass Spectrometry Laboratory (PMSL), a collaborative core laboratory within the Semel Institute at UCLA. The PMSL does not currently have an instrument suitable for liquid chromatography/mass spectrometry (LC/MS) metabolomics or proteomics experiments. Such an instrument is essential to support the range of research projects in which the PMSL is involved and for which it has earned high regard. Acquisition of the requested equipment will ensure that the PMSL and its collaborating laboratories remain competitive in their respective fields and continue to make progress on the many important and exciting research projects on which they are working. The projects for which the requested instrument will be used encompass basic, translational, and clinical sciences, involving cutting-edge research in molecular biomarker screening for a number of diseases including Alzheimer's Disease and related disorders, a variety of cancers, and iron deficiency anemia, among others. The compounds that are being identified and quantified in these experiments are implicated in diverse biological phenomena including signal transduction, cellular communication, hormonal homeostasis, the addictive process, aging, immune responses, and inflammation. This application includes descriptions of projects from 23 collaborating laboratories: 6 are designated as Major and the remainder as Minor Projects, the basis of this distinction being overall time-commitment of instrument and personnel, all equally important and exciting. These collaborations are expected to occupy approximately 90% of instrument time. The remaining instrument time will be available to other collaborating groups for short-term exploratory research. The PMSL has the infrastructure to maintain and support the new equipment backed by an outstanding track record fostering the application of contemporary mass spectrometry to address important biological problems in science and medicine. Professor Faull, the PI on this application and the Director of the PMSL, Professor Whitelgge, de facto deputy director of the PMSL, and Research Assistants Joseph Capri and Farbod Fazlollahi, will be responsible for the day-to-day operation and maintenance of the new equipment. Professors Faull and Whitelegge have an exceptional reputation for the effective use of equipment purchased through the NIH SIG mechanism. This application represents a critical component of the effort by UCLA, the David Geffen School of Medicine, and The Semel Institute, to equip collaborative campus core laboratories with state-of-the- art instruments for cutting-edge research in metabolomics, proteomics, and related fields.