Purchase of Mass Spectrometric Instrumentation

购买质谱仪器

• 批准号: 6730903
• 负责人: KYM Francis FAULL
• 金额: $ 34.75万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730903
• 关键词: biomedical equipment purchase; liquid chromatography mass spectrometry

DESCRIPTION (provided by applicant): Funds are requested to purchase a high performance LC/MS/MS system equipped with electrospray, nanospray and atmospheric pressure chemical ionization sources for the UCLA Pasarow Mass Spectrometry Laboratory. This is a shared facility jointly supported by the Department of Chemistry & Biochemistry (College of Letters and Sciences) and the Department of Psychiatry & Biobehavioral Sciences and the Neuropsychiatric Institute (School of Medicine) that is available to the entire UCLA campus research and teaching enterprise. The driving force behind this application is the need for an instrument that can be devoted to analyses of brain dialysate tissue perfusion biopsy samples for the specific quantification of multiple neurotransmitters and related compounds in the same samples, and for the identification of novel neuro-regulatory compounds, which are co-released with known neurotransmitters. At least seven different laboratories on the UCLA campus will use this instrument in their research. The extreme demands for sensitivity in this research require an instrument that can be reserved for operation at absolute maximal efficiency. There is no instrument on the UCLA campus, which is available and suitable for this type of research and these types of samples. This instrument will be a central analytical tool of the growing cadre of UCLA scientists who use brain dialysis as a core experimental technique, and the acquisition of this instrument is essential for the continued productivity, success and competitiveness of these programs. The PI and his associates have carefully evaluated all available instruments which potentially fulfill the demanding requirements, and have chosen the Q Trap Pro LC/MS/MS system package from Applied Biosystems as the most suitable of those currently available. Descriptions of the research of the seven major users of the instrument are provided.

描述（由申请人提供）： 申请资金为加州大学洛杉矶分校帕萨罗质谱实验室购买配备电喷雾、纳喷雾和大气压化学电离源的高性能 LC/MS/MS 系统。这是一个由化学与生物化学系（文学与科学学院）、精神病学与生物行为科学系以及神经精神病学研究所（医学院）共同支持的共享设施，可供整个加州大学洛杉矶分校校园的研究和教学企业使用。该应用背后的驱动力是需要一种能够专门分析脑透析液组织灌注活检样本的仪器，以对同一样本中的多种神经递质和相关化合物进行特定定量，并鉴定与已知神经递质共同释放的新型神经调节化合物。加州大学洛杉矶分校校园内至少有七个不同的实验室将在其研究中使用该仪器。这项研究对灵敏度的极端要求需要一种能够以绝对最大效率运行的仪器。加州大学洛杉矶分校校园内没有可用且适合此类研究和此类样本的仪器。该仪器将成为加州大学洛杉矶分校日益壮大的科学家队伍的核心分析工具，他们使用脑透析作为核心实验技术，而获得该仪器对于这些项目的持续生产力、成功和竞争力至关重要。 PI 及其同事仔细评估了所有可能满足严格要求的可用仪器，并选择 Applied Biosystems 的 Q Trap Pro LC/MS/MS 系统包作为当前可用的最合适的系统包。提供了该仪器的七个主要用户的研究描述。