Thermo Vantage triple quadrupole with FAIMS and Accela UPLC

配备 FAIMS 和 Accela UPLC 的 Thermo Vantage 三重四极杆

• 批准号: 7795994
• 负责人: KYM Francis FAULL
• 金额: $ 49.45万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-07-10 至 2011-07-09
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7795994
• 关键词: 17 year old; Age; Aging-Related Process; Alzheimer' s Disease; Arts; Blood capillaries; Brain Injuries; Communication; Complex Mixtures; Computers; Detection; Development; Disease; Doctor of Philosophy; Equipment; Frequencies; Funding; Funding Applicant; Gas Chromatography; Health; High Pressure Liquid Chromatography; Homeostasis; Hormonal; Human; Immune response; Inflammation; Institutes; Ions; Laboratories; Liquid substance; Maintenance; Malignant Neoplasms; Mass Spectrum Analysis; Mental disorders; Metabolic Diseases; Molecular; Molecular Weight; Parkinson Disease; Personal Satisfaction; Population; Process; Research; Research Infrastructure; Research Project Grants; Resolution; Signal Transduction; Societies; Spectrometry; System; Technology; Time; United States National Institutes of Health; Viral; Work; age related; base; capillary; flexibility; instrument; ion mobility; liquid chromatography mass spectrometry; mass spectrometer; medical schools; metabolomics; nervous system disorder; neuropsychiatry; operation; professor; public health relevance; ultra high pressure

DESCRIPTION (provided by applicant): Funds are requested for a state-of-the-art triple quadrupole mass spectrometer (QqQ) and two essential ancillary attachments - an ultra-high pressure liquid chromatography (UPLC) system, and a High- Field Assymetric Waveform Ion Mobility Spectrometry (FAIMS) attachment. This equipment will be located in the Pasarow Mass Spectrometry Laboratory (PMSL) within the Semel (formerly Neuropsychiatric) Institute at UCLA, a campus-wide core mass spectrometry laboratory. The QqQ mass spectrometers currently in the PMSL are about 17 years old and these are obsolete by any criteria. In the intervening period there have been significant improvements in sensitivity, resolution, accuracy of m/z assignment, computer control, experimental flexibility, and detection dynamic range of these instruments. UPLC brings liquid chromatographic resolution close to that attainable with capillary gas chromatography, and is an important development for high sensitivity work, high throughput work, and for work with complex mixtures. There is no UPLC system on the UCLA campus. The FAIMS technology is a new development that for some compounds lowers the background from isobaric ions, and as such can provide a significant advantage for low molecular weight analyses of complex mixtures by LC/MS, as is intended for the new instrument. The research projects for which the equipment will be used involve quantitative and qualitative analyses of a diverse range of molecules, many but not all with relatively low molecular weights (generally less that 1500 Da), in a variety of milieu. Collectively these compounds are implicated in diverse phenomena including signal transduction, cellular communication, hormonal homeostasis, addictive processes, ageing, logical thought processing, immune responses and inflammation. The application includes descriptions of eighteen collaborating laboratories with mass spectrometric analysis essential to their research projects. These groups are expected to occupy approximately 90% of instrument time. The remaining instrument time will be used by other collaborating groups as needed. Funding support is in place for all the collaborating laboratories. The PMSL has the necessary infrastructure to maintain and support the new equipment. Professor Faull, the PI on this application and the Director of the PMSL, and Postdoctoral Research Fellow Christopher Ryan, PhD, will be responsible for the day-to-day operation and maintenance of the new equipment. The PI has a strong track- record for the effective use of equipment purchased through the NIH SIG mechanism. This application represents a critical component of an effort by the UCLA Semel Institute and School of Medicine to re-place obsolete equipment within the campus core laboratories with state-of-the-art instruments for cutting edge research in metabolomics and related fields. PUBLIC HEALTH RELEVANCE: The research for which the new equipment will be used is directly relevant to many aspects of human health and disease, including cancer, metabolic diseases, brain injury, viral infectivity, psychiatric diseases, and age-related neurological diseases including Alzheimer's and Parkinson's diseases. The frequency with which many of these diseases occur in Western society is rising as the population ages. Research equipment such as that requested is crucial for understanding the molecular basis of these diseases and continued progress on these important problems that significantly impact human health and well-being.

描述（由申请人提供）：申请资金用于最先进的三重四极杆质谱仪（QqQ）和两个基本辅助附件-超高压液相色谱（UPLC）系统和高场不对称波形离子迁移谱仪（FAIMS）附件。该设备将位于加州大学洛杉矶分校塞梅尔（原神经精神病学）研究所内的帕萨罗质谱实验室（PMSL），这是一个校园范围的核心质谱实验室。目前在PMSL的QqQ质谱仪大约有17年的历史，按照任何标准都是过时的。在此期间，这些仪器的灵敏度、分辨率、m/z分配的准确性、计算机控制、实验灵活性和检测动态范围都有了显着的改善。UPLC使液相色谱分离度接近毛细管气相色谱法，是高灵敏度工作、高通量工作和复杂混合物工作的重要发展。UCLA校园内没有UPLC系统。FAIMS技术是一项新的发展，对于某些化合物，它降低了来自同量异位素离子的背景，因此可以为LC/MS对复杂混合物的低分子量分析提供显着优势，这是新仪器的目的。将使用该设备的研究项目涉及在各种环境中对各种分子进行定量和定性分析，其中许多分子（但不是所有分子）的分子量相对较低（通常小于1500 Da）。总的来说，这些化合物涉及多种现象，包括信号转导、细胞通讯、激素稳态、成瘾过程、衰老、逻辑思维处理、免疫反应和炎症。该应用程序包括18个合作实验室的描述，质谱分析对其研究项目至关重要。预计这些组将占用约90%的仪器时间。剩余的仪器时间将根据需要由其他合作小组使用。为所有合作实验室提供了资金支持。PMSL拥有必要的基础设施来维护和支持新设备。Faull教授，本申请的PI和PMSL主任，博士后研究员Christopher Ryan博士，将负责新设备的日常操作和维护。PI在有效使用通过NIH SIG机制购买的设备方面有着良好的记录。该应用程序代表了加州大学洛杉矶分校塞梅尔研究所和医学院努力更换校园核心实验室内的过时设备的关键组成部分，这些设备具有最先进的仪器，用于代谢组学和相关领域的尖端研究。 公共卫生相关性：新设备将用于的研究与人类健康和疾病的许多方面直接相关，包括癌症，代谢性疾病，脑损伤，病毒感染，精神疾病以及与年龄相关的神经系统疾病，包括阿尔茨海默氏症和帕金森氏症。在西方社会，随着人口老龄化，这些疾病的发生频率也在上升。所要求的研究设备对于了解这些疾病的分子基础和在这些严重影响人类健康和福祉的重要问题上继续取得进展至关重要。