The Dictyostelium Stock Center

 描述(申请人提供)：真核生物盘基网柄菌是一种模式生物，广泛用于研究细胞过程，如细胞运动、趋化性、信号转导、细胞对药物的反应以及宿主与病原体的相互作用。网柄菌的基因组包括与脊椎动物、酵母和微生物基因有显著同源性的基因，吸引了研究人员对广泛的生物学主题感兴趣，如人类疾病、比较基因组学和自我/非自我识别。Dictyostatus研究界从Dicty Stock Center(DSC)中受益匪浅，该中心是菌株和质粒的中央储存库，提供了一种成本效益高的手段，确保菌株广泛可用并在标准化条件下维护。DSC使新的网柄网柄菌研究人员能够获得经过测试的研究材料，并以最低的成本快速启动新的研究项目。知识渊博的DSC工作人员为有效处理菌株提供技术支持和方案。DSC还大大受益于它与DictyBase的协同集成，DictyBase是Dictyostelials的模式生物数据库(MOD)，它提供了菌株和质粒注释的网络界面，并允许在线提交菌株订单。DSC收集了含有新表型的网柄基菌和其他种类的社会阿米巴的转基因菌株，这些品种很难重新设计。它还提供了来自经验丰富的研究人员的表达和基因靶向质粒，这将是重建所需的时间。此应用程序寻求支持以继续运行此社区资源。这项建议的目标有两个：(1)通过继续收集、注释和分发DSC集合，继续收集、注释和分发DSC集合，重点关注产生表型和标记菌株(如GFP融合)的突变株以及遵循不断更新的标准操作程序的有用的质粒、cDNA文库和抗体；(2)创建MyDSC，通过创建个性化的用户帐户，允许用户更改、更新或删除他们的订单，请求不可用的菌株，并为每个菌株留下关于生长习性或表型的评论；该系统还将便利用户和DSC工作人员之间的交流，简化双方的工作流程。实施这些改进将提高DSC工作人员的效率，并极大地改善用户的在线体验。

 DESCRIPTION (provided by applicant): The eukaryote Dictyostelium discoideum is a model organism widely used for the study of cellular processes such as cell motility, chemotaxis, signal transduction, cellular response to drugs, and host-pathogen interactions. Dictyostelium's genome includes genes with significant homology to vertebrate, yeast and microbial genes, attracting researchers interested in a wide variety of biological topics such as human disease, comparative genomics, and self/non-self-recognition. The Dictyostelium research community has significantly benefited from the Dicty Stock Center (DSC), a central repository of strains and plasmids that provides a cost-effective means of ensuring that strains are widely available and maintained in standardized conditions. The DSC enables new Dictyostelium researchers to obtain tested research materials and quickly start new research projects at minimal costs. The knowledgeable DSC staff provides technical support and protocols for effective handling of the strains. The DSC also benefits greatly from its synergistic integration with dictyBase, the model organism database (MOD) for Dictyostelium, which provides the web interface for strain and plasmid annotations, and allows strain orders to be submitted online. The DSC collects genetically modified strains of Dictyostelium and other species of social amoebae that contain novel phenotypes and which would be difficult to re-engineer. It also offers both expression and gene-targeting plasmids from experienced researchers that would be time consuming to reconstruct. This application seeks support to continue operating this community resource. The goals of this proposal are twofold: (1) Maintain, expand, and distribute the DSC collection by continuing to collect, annotate and distribute strains from Dictyostelium and related organisms, focusing on mutants that produce phenotypes and marked strains (such as GFP fusions) as well as useful plasmids, cDNA libraries, and antibodies following continuously updated standard operating procedures; (2) Create MyDSC to improve usability and utility for users by creating personalized user accounts, allowing users to change, update or delete their orders, request unavailable strains, and to leave comments pertaining to growth habits or phenotypes for each strain; this system will also facilitate communications between users and DSC staff, easing the workflow on both sides. Implementing these improvements will increase efficiency for DSC staff and greatly improve the online experience for users.