COBRE: Smooth Muscle Plasticity

描述(申请人提供)：在第三阶段科布雷，我们将继续发展的主题重点：平滑肌可塑性，通过继续和加强科布雷阶段的核心实验室服务L和11。平滑肌是包含多种细胞类型的复杂组织。将这些肌肉作为同质组织处理一直是评估基因表达以及在发育和衰老、糖尿病、重塑、炎症反应等情况下基因表达变化的常见方法。内华达大学的平滑肌肉生物学小组开创了使用细胞特异性报告程序和表面抗原对平滑肌组织的细胞成分进行分类，然后对细胞特异性转录进行全基因组评估的技术。目前正在使用Fluidigm BioMark HD技术评估特定细胞类型中非常突出的基因，以确定随着平滑肌组织在发育或疾病状态下发生变化，基因表达的逐个细胞的定量变化。转录分析还伴随着使用核糖体分析技术对哪些转录本进行积极翻译的评估。我们还在创造特定基因上调或下调的细胞系。COBRE的主题重点服务将由核心B(FACS/流式细胞术共享资源实验室)和核心C(单细胞分子表达)提供。提供了详细的计划，以加强整个大学这些核心的用户基础，并为该州的研究界创建长期、可持续的核心设施。第三阶段将由一项试点资助计划补充，该计划旨在通过大学内的几个更多项目增加核心的使用量。试点赠款将授予合格的调查员，以利用核心技术获得试点数据，以便核心的利用将增强赠款提案的力度，并在为新的赠款提供资金时增加核心使用量。第三阶段由一个行政核心(核心A)领导，该核心通过首席调查员和计划协调员提供领导，通过内部指导委员会进行质量控制，并通过评估协调员和外部咨询委员会进行年度评估。

DESCRIPTION (provided by applicant): In this Phase III COBRE we will continue to develop the thematic focus: Smooth Muscle Plasticity, by continuing and enhancing Core laboratory services developed during COBRE Phases l&ll. Smooth muscles are complex tissues containing multiple cell types. Treating these muscles as homogenous tissues has been a common way to evaluate gene expression and the changes that occur in gene expression in conditions such as development and aging, diabetes, remodeling, responses to inflammation, etc. The smooth muscle biology group at the University of Nevada has pioneered techniques to sort the cellular components of smooth muscle tissues using cell-specific reporters and surface antigens and then to perform genome wide evaluations of cell-specific transcriptomes. Highly prominent genes in specific cell types are being evaluated with the Fluidigm Biomark HD technology to determine cell-by-cell quantitative changes in gene expression as smooth muscle tissues are altered in development or disease states. Transciptional analysis is also accompanied by evaluation of which transcripts are actively translated using Ribosome profiling techniques. We are also creating cell lines in which specific genes are up or down regulated. Services to the thematic focus of the COBRE will be provided by Core B (FACS/flow cytometry Shared Resource Lab) and Core C (Single Cell Molecular Expression). Detailed plans are provided to enhance the user base for these Cores throughout the University and to create long-lasting sustainable Core facilities for the research community in the State. The Phase III will be supplement by a Pilot Grant Program that is designed to increase usage of the Cores by several more projects within the University. Pilot Grants will be awarded to qualified investigators to obtain pilot data using Core technologies such that utilization of the Cores will enhance the strength of grant proposals and increase Core usage when new grants are funded. The Phase III is lead by an Administrative Core (Core A) which provides leadership via the Principal Investigator and Program Coordinator, quality control via an Internal Steering Committee, and annual evaluation through an Assessment Coordinator and an External Advisory Committee.