COBRE: Smooth Muscle Plasticity

COBRE：平滑肌可塑性

• 批准号: 9115199
• 负责人: KENTON M SANDERS
• 金额: $ 107.62万
• 依托单位: UNIVERSITY OF NEVADA RENO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2019-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9115199
• 关键词: Advisory Committees; Aging; Applications Grants; Award; Behavior; Biology; Cell Line; Cells; Centers of Research Excellence; Communities; Complex; Core Facility; Data; Development; Diabetes Mellitus; Disease; Evaluation; Flow Cytometry Shared Resource; Funding; Gene Expression; Genes; Grant; Inflammation; Laboratories; Lead; Leadership; Molecular; Muscle; Nevada; Phase; Pilot Projects; Qualifying; Quality Control; Reporter; Research; Research Personnel; Research Project Grants; Services; Smooth Muscle; Sorting - Cell Movement; Surface Antigens; Techniques; Technology; Tissues; Transcript; Translating; Universities; base; cell type; design; genome-wide; programs; response; ribosome profiling; transcriptome

In this Phase III COBRE we will continue to develop the thematic focus: Smooth Muscle Plasticity, by continuing and enhancing Core laboratory services developed during COBRE Phases I&II. Smooth muscles are complex tissues containing multiple cell types. Treating these muscles as homogenous tissues has been a common way to evaluate gene expression and the changes that occur in gene expression in conditions such as development and aging, diabetes, remodeling, responses to inflammation, etc. The smooth muscle biology group at the University of Nevada has pioneered techniques to sort the cellular components of smooth muscle tissues using cell-specific reporters and surface antigens and then to perform genome wide evaluations of cell-specific transcriptomes. Highly prominent genes in specific cell types are being evaluated with the Fluidigm Biomark HD technology to determine cell-by-cell quantitative changes in gene expression as smooth muscle tissues are altered in development or disease states. Transciptional analysis is also accompanied by evaluation of which transcripts are actively translated using Ribosome profiling techniques. We are also creating cell lines in which specific genes are up or down regulated. Services to the thematic focus of the COBRE will be provided by Core B (FACS/flow cytometry Shared Resource Lab) and Core C (Single Cell Molecular Expression). Detailed plans are provided to enhance the user base for these Cores throughout the University and to create long-lasting sustainable Core facilities for the research community in the State. The Phase III will be supplement by a Pilot Grant Program that is designed to increase usage of the Cores by several more projects within the University. Pilot Grants will be awarded to qualified investigators to obtain pilot data using Core technologies such that utilization of the Cores will enhance the strength of grant proposals and increase Core usage when new grants are funded. The Phase III is lead by an Administrative Core (Core A) which provides leadership via the Program Director and Program Coordinator, quality control via an Internal Steering Committee, and annual evaluation through an Assessment Coordinator and an External Advisory Committee.

在这一阶段III索布雷，我们将继续发展主题焦点：平滑肌可塑性， 在I＆II期间持续和增强核心实验室服务。平滑的肌肉 是包含多种细胞类型的复杂组织。将这些肌肉视为同质组织 是评估基因表达和基因表达中发生的变化的常见方法 诸如发育和衰老，糖尿病，重塑，对炎症的反应等疾病等。 内华达大学的平滑肌生物学组具有先驱技术来对细胞进行分类 使用细胞特异性记者和表面抗原的平滑肌组织成分，然后执行 基因组对细胞特异性转录组的广泛评估。特定细胞类型中高度突出的基因是 通过流体生物摩变高清技术评估，以确定逐个细胞的定量变化 基因表达作为平滑肌组织在发育或疾病状态中发生了改变。触发分析还伴随着评估哪些转录本使用核糖体进行了积极翻译 分析技术。我们还创建了细胞系，其中特定基因被调节。 Core B（FACS/流式细胞术共享 资源实验室）和核C（单细胞分子表达）。提供了详细的计划来增强 这些核心的用户群在整个大学中 该州的研究界。第三阶段将由试点赠款计划进行补充 旨在通过大学内的其他几个项目来增加对核心的使用。飞行员赠款将是 授予合格的调查人员使用核心技术获取试验数据，以便利用 核心将提高赠款提案的实力，并在资助新赠款时增加核心使用情况。 第三阶段是由行政核心（核心A）领导的，该核心通过计划主任提供领导 和计划协调员，通过内部指导委员会进行质量控制，以及通过年度评估 评估协调员和外部咨询委员会。