Smooth Muscle Plasticity: A COBRE

描述（由申请人提供）：本提案旨在为内华达大学里诺分校（UNR）的卓越生物医学研究平滑肌可塑性中心（COBRE）更新资金。COBRE扩大了研究基础设施，并帮助发展了几位非常有前途的年轻研究人员的职业生涯。该中心在平滑肌生物学方面有很强的专题重点。COBRE由5个项目组成，研究平滑肌可塑性的各个方面。项目1：糖尿病性胃轻瘫的结构与运动缺陷的相关性，由Grant Hennig博士牵头；项目二：平滑肌可塑性中的磷蛋白和CaM激酶II，由Brian Perrino博士牵头；项目3：确定平滑肌力学调节机制的体外模型系统，由Josh Baker博士领导；项目4：由Dr. Seungil Ro领导的microRNAs及其靶基因调控的平滑肌肥大；项目5：由Sang Don Koh博士领导的过度活动膀胱拉伸依赖性钾通道调节。这些项目由核心实验室设施提供支持：核心A：管理和教师发展；核心B：分子表达和转基因；核心C：蛋白表达和细胞形态；Core D：动态成像。COBRE将由以下部门管理：i) COBRE中央行政部门，由PI和Co-PI、行政助理和计算机专家组成；ii)内部咨询委员会（lAC），为每个项目负责人配备导师；iii)由平滑肌生物学领域的领导者组成的外部咨询委员会（EAC）。lAC的导师是杰出的科学家，在生物医学研究领域具有丰富的职业生涯。lAC为职业发展制定里程碑，对里程碑的进展进行形成性和总结性评估，并协助项目负责人实现发展独立资助和可持续研究事业的中心目标。COBRE由dr。肯顿·桑德斯和克里斯汀·克雷莫分别担任PI和Co-PI。PI和Co-PI都是非常合格的，在COBRE的主题重点方面具有多年的行政和科学经验。

DESCRIPTION (provided by applicant): This proposal is to renew funding for the Smooth Muscle Plasticity Center of Biomedical Research Excellence (COBRE) at the University of Nevada, Reno (UNR). The COBRE has expanded the research infrastructure and helped to develop the careers of several highly promising young investigators. The center has a strong thematic focus in smooth muscle biology. The COBRE consists of 5 projects that are investigating various aspects of smooth muscle plasticity. Project 1: Correlation between structural and motor defects in diabetic gastroparesis to be headed by Dr. Grant Hennig; Project 2: Phopholamban and CaM Kinase II in smooth muscle plasticity to be headed by Dr. Brian Perrino; Project 3: An in vitro model system for determining regulatory mechanisms for smooth muscle mechanics to be led by Dr. Josh Baker; Project 4: Smooth muscle hypertrophy regulated by microRNAs and their target genes to be headed by Dr. Seungil Ro; Project 5: Stretch-dependent potassium channel regulation in overactive bladder to be led by Dr. Sang Don Koh. The projects are supported by Core lab facilities: Core A: Administration and faculty development; Core B: Molecular expression and transgenics; Core C: Protein expression and cell morphology; and Core D: Dynamic imaging. The COBRE will be administered by: i) the Central COBRE Administration consisting of the PI and Co-PI, administrative assistant and computer specialist; ii) an Internal Advisory Committee (lAC) with mentors for each project leader; and iii) an External Advisory Committee (EAC) consisting of leaders in smooth muscle biology. Mentors in the lAC are distinguished scientists with productive careers in biomedical research. The lAC establishes milestones for career development, performs formative and summative evaluation of progress toward milestones, and assists the Project Leaders in the central goal of developing independent funding and sustainable research careers. The COBRE is led by Drs. Kenton Sanders and Christine Cremo as PI and Co-PI, respectively. The PI and Co-PI are highly qualified, with many years of administrative and scientific experience in the thematic focus of the COBRE. PUBLIC HEALTH RELEVANCE (provided by applicant): Smooth muscles are unique among muscle lineages because they change phenotype in response to a variety of stimuli. Pathophysiological conditions result from phenotypic changes in smooth muscle tissues, but the cause and consequences of remodeling and hypertrophy are not well understood. Several disease models of smooth muscles will be used to learn how phenotypic change contributes to pathophysiology.