Study of Accessory Lacrimal Gland and Precursor Cell Biology

副泪腺及前体细胞生物学研究

• 批准号: 8679183
• 负责人: Vinay Aakalu
• 金额: $ 23.64万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8679183
• 关键词: ABCG2 gene; Address; Affect; Age; Anatomy; Atrophic; Basic Science; Biology; Blindness; Cell Separation; Cell Survival; Cells; Cellular biology; Characteristics; Chicago; Clinical; Clinical Investigator Award; Development; Disease; Ethics; Eye; Eye diseases; Film; Fluorescence; Gender; Gland; Goals; Growth; Harvest; Health; Histologic; Hormonal; Human; Illinois; Immunohistochemistry; Investigation; K-Series Research Career Programs; Knowledge; Lacrimal gland structure; Lead; Location; Lubrication; Maintenance; Maps; Mentors; Microscopic; Molecular; Mus; Ocular orbit; Operative Surgical Procedures; Patients; Pattern; Performance; Persons; Population; Production; Productivity; Property; Quality of life; Race; Regenerative Medicine; Research; Research Personnel; Scientist; Specimen; Stem cells; Surface; Techniques; Thinking; Tissue Microarray; Tissues; Training; Translational Research; Transplantation; Treatment Cost; Universities; Work; age effect; aqueous; base; career; chronic pain; clinical practice; corneal scar; experience; eye dryness; lacrimal; ocular surface; older patient; precursor cell; programs; public health relevance; research and development; skills; stem cell biology; symposium; translational clinical trial; vision science

Project Abstract This is a proposal for a K08 Mentored Clinical Scientist Career Development Award to train Dr. Vinay Kumar Aakalu. The primary goal of this application is to serve as a basis for training Dr. Aakalu in the critical skills necessary to develop into an independent scientific investigator focused on lacrimal cell and precursor cell biology. The specific aims of the proposed educational program are the performance of 1) graduate level cell, molecular, stem cell biology and basic translational/clinical trial course work at the University of Illinois at Chicago 2) basic scientific investigations in accessory lacrimal cell and stem cell biology under the guidance of an experienced four person mentoring committee 3) seminar and conference participation at the University of Illinois and outside the University of Illinois. The mentoring committee is made up of experts in stem cell, ocular, molecular cell biology and translational research. Each mentor is well experienced in training clinician- scientists. The mentoring committee will be focused on guiding Dr. Aakalu in the development of research skills, analytical thinking and the ethical performance of basic and translational research. The overarching research goals of the proposal are to develop a better understanding of accessory lacrimal cell biology and to identify and isolate precursor cells from this tissue. The specific aims of the project are to: 1. Develop a better understanding of ALG distribution, growth requirements, and changes with patient characteristics. 1a. A three dimensional map of the cadaveric human orbit will be constructed to determine the location of ALG tissue throughout the ocular surface as a basis for rational harvesting decisions. 1b. Characterize ALG tissue in human surgical specimens and relate the robustness or histopathologic changes in these tissues to relevant patient characteristics (age, gender, race, hormonal status). 1c. Develop ALG cultures from human surgical specimens to better understand requirements for cell survival and expansion. 2. Establish the presence of precursor cells in ALG tissue and isolate and characterize these precursor cells. 2a. Identify precursor marker positive ALG cells and determine a signature marker pattern using human specimens and immunohistochemistry on tissue microarrays. 2b. Isolate precursor candidate cells utilizing fluorescence cell sorting and determine if changes in precursor candidate cell populations are noted with different patient characteristics. 2c. Determine if precursor candidate cells isolated have stem cell properties. 2d. Establish cultures from precursor candidates and determine if these cultures can produce functional ALG cells.

项目摘要 这是为培训Vinay Kumar博士而设立的K08临床科学家导师职业发展奖的建议 阿卡鲁。此应用程序的主要目标是作为培训Aakalu博士关键技能的基础 有必要发展成为专注于泪液细胞和前体细胞的独立科学研究人员 生物学。拟议的教育计划的具体目标是1)研究生水平单元的表现， 伊利诺伊大学分子、干细胞生物学和基础翻译/临床试验课程工作 芝加哥2)辅助泪液细胞和干细胞生物学的基础科学研究 一位经验丰富的四人指导委员会3)在加州大学参加研讨会和会议 伊利诺伊州和伊利诺伊大学外。指导委员会由干细胞方面的专家组成， 眼科、分子细胞生物学和翻译研究。每一位导师都在培训临床医生方面经验丰富- 科学家们。指导委员会将专注于指导Aakalu博士的研究开发 基础研究和翻译研究的技能、分析思维和道德表现。最重要的是 该提案的研究目标是更好地了解副泪腺细胞生物学，并 从该组织中鉴定并分离前体细胞。该项目的具体目标是：1.开发一种 更好地了解ALG的分布、生长要求和随患者特征的变化。1A.一个 将构建身体人体眼眶的三维地图，以确定ALG的位置 整个眼表的组织，作为合理的收获决定的基础。1B.ALG组织的特征 并将这些组织中的稳健性或组织病理学变化与相关 患者特征(年龄、性别、种族、荷尔蒙状况)。1C。从人类外科发展ALG培养 标本，以更好地了解细胞生存和扩张的要求。2.确定……存在 ALG组织中的前体细胞，分离和鉴定这些前体细胞。2A。识别前驱标记 阳性的ALG细胞，并使用人类标本和 组织芯片上的免疫组织化学。2B。利用荧光细胞分离前体候选细胞 分类并确定不同患者是否注意到前体候选细胞群的变化 特点。2C。确定分离的前体候选细胞是否具有干细胞特性。2D。建立 从前体候选细胞中进行培养，并确定这些培养物是否能产生功能性ALG细胞。