Study of Accessory Lacrimal Gland and Precursor Cell Biology

副泪腺及前体细胞生物学研究

• 批准号: 8679183
• 负责人: Vinay Aakalu
• 金额: $ 23.64万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8679183
• 关键词: ABCG2 gene; Address; Affect; Age; Anatomy; Atrophic; Basic Science; Biology; Blindness; Cell Separation; Cell Survival; Cells; Cellular biology; Characteristics; Chicago; Clinical; Clinical Investigator Award; Development; Disease; Ethics; Eye; Eye diseases; Film; Fluorescence; Gender; Gland; Goals; Growth; Harvest; Health; Histologic; Hormonal; Human; Illinois; Immunohistochemistry; Investigation; K-Series Research Career Programs; Knowledge; Lacrimal gland structure; Lead; Location; Lubrication; Maintenance; Maps; Mentors; Microscopic; Molecular; Mus; Ocular orbit; Operative Surgical Procedures; Patients; Pattern; Performance; Persons; Population; Production; Productivity; Property; Quality of life; Race; Regenerative Medicine; Research; Research Personnel; Scientist; Specimen; Stem cells; Surface; Techniques; Thinking; Tissue Microarray; Tissues; Training; Translational Research; Transplantation; Treatment Cost; Universities; Work; age effect; aqueous; base; career; chronic pain; clinical practice; corneal scar; experience; eye dryness; lacrimal; ocular surface; older patient; precursor cell; programs; public health relevance; research and development; skills; stem cell biology; symposium; translational clinical trial; vision science

Project Abstract This is a proposal for a K08 Mentored Clinical Scientist Career Development Award to train Dr. Vinay Kumar Aakalu. The primary goal of this application is to serve as a basis for training Dr. Aakalu in the critical skills necessary to develop into an independent scientific investigator focused on lacrimal cell and precursor cell biology. The specific aims of the proposed educational program are the performance of 1) graduate level cell, molecular, stem cell biology and basic translational/clinical trial course work at the University of Illinois at Chicago 2) basic scientific investigations in accessory lacrimal cell and stem cell biology under the guidance of an experienced four person mentoring committee 3) seminar and conference participation at the University of Illinois and outside the University of Illinois. The mentoring committee is made up of experts in stem cell, ocular, molecular cell biology and translational research. Each mentor is well experienced in training clinician- scientists. The mentoring committee will be focused on guiding Dr. Aakalu in the development of research skills, analytical thinking and the ethical performance of basic and translational research. The overarching research goals of the proposal are to develop a better understanding of accessory lacrimal cell biology and to identify and isolate precursor cells from this tissue. The specific aims of the project are to: 1. Develop a better understanding of ALG distribution, growth requirements, and changes with patient characteristics. 1a. A three dimensional map of the cadaveric human orbit will be constructed to determine the location of ALG tissue throughout the ocular surface as a basis for rational harvesting decisions. 1b. Characterize ALG tissue in human surgical specimens and relate the robustness or histopathologic changes in these tissues to relevant patient characteristics (age, gender, race, hormonal status). 1c. Develop ALG cultures from human surgical specimens to better understand requirements for cell survival and expansion. 2. Establish the presence of precursor cells in ALG tissue and isolate and characterize these precursor cells. 2a. Identify precursor marker positive ALG cells and determine a signature marker pattern using human specimens and immunohistochemistry on tissue microarrays. 2b. Isolate precursor candidate cells utilizing fluorescence cell sorting and determine if changes in precursor candidate cell populations are noted with different patient characteristics. 2c. Determine if precursor candidate cells isolated have stem cell properties. 2d. Establish cultures from precursor candidates and determine if these cultures can produce functional ALG cells.

项目摘要 这是一项 K08 指导临床科学家职业发展奖的提案，旨在培训 Vinay Kumar 博士 阿卡鲁。此应用程序的主要目标是作为培训 Aakalu 博士关键技能的基础 发展成为专注于泪腺细胞和前体细胞的独立科学研究者所必需的 生物学。拟议教育计划的具体目标是 1) 研究生水平单元的表现， 伊利诺伊大学分子、干细胞生物学和基础转化/临床试验课程 芝加哥2）在副泪腺细胞和干细胞生物学的指导下进行基础科学研究 经验丰富的四人指导委员会 3) 参加大学的研讨会和会议 伊利诺伊州和伊利诺伊大学外。指导委员会由干细胞领域专家组成， 眼科、分子细胞生物学和转化研究。每位导师在培训临床医生方面都拥有丰富的经验- 科学家。指导委员会将重点指导Aakalu博士的研究发展 技能、分析思维以及基础和转化研究的道德表现。首要的 该提案的研究目标是更好地了解副泪腺细胞生物学并 从该组织中识别并分离前体细胞。该项目的具体目标是： 1. 开发一个 更好地了解 ALG 分布、生长要求以及患者特征的变化。 1a.一个 将构建人体尸体轨道三维图以确定 ALG 的位置 整个眼表组织作为合理采集决策的基础。 1b.表征 ALG 组织 人类手术标本并将这些组织的稳健性或组织病理学变化与相关 患者特征（年龄、性别、种族、荷尔蒙状态）。 1c.从人类手术中开发 ALG 培养物 样本以更好地了解细胞存活和扩增的要求。 2. 确定存在 ALG 组织中的前体细胞，并分离和表征这些前体细胞。 2a.识别前体标记 阳性 ALG 细胞并使用人类样本确定特征标记模式 组织微阵列上的免疫组织化学。 2b.利用荧光细胞分离前体候选细胞 分选并确定不同患者是否注意到前体候选细胞群的变化 特征。 2c.确定分离的前体候选细胞是否具有干细胞特性。 2d.建立 来自前体候选物的培养物，并确定这些培养物是否可以产生功能性 ALG 细胞。