Study of Accessory Lacrimal Gland and Precursor Cell Biology

副泪腺及前体细胞生物学研究

• 批准号: 9044784
• 负责人: Vinay Aakalu
• 金额: $ 23.64万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9044784
• 关键词: ABCG2 gene; Address; Affect; Age; Anatomy; Atrophic; Basic Science; Biology; Blindness; Cell Separation; Cell Survival; Cells; Cellular biology; Characteristics; Chicago; Clinical; Clinical Investigator Award; Development; Disease; Ethics; Eye; Eye diseases; Film; Fluorescence; Gender; Gland; Goals; Growth; Harvest; Health; Histologic; Hormonal; Human; Illinois; Immunohistochemistry; Investigation; K-Series Research Career Programs; Knowledge; Lacrimal gland structure; Lead; Location; Lubrication; Maintenance; Maps; Mentors; Microscopic; Molecular; Mus; Ocular orbit; Operative Surgical Procedures; Patients; Pattern; Performance; Persons; Population; Production; Productivity; Property; Quality of life; Race; Regenerative Medicine; Research; Research Personnel; Scientist; Specimen; Stem cells; Surface; Techniques; Thinking; Tissue Microarray; Tissues; Training; Translational Research; Transplantation; Treatment Cost; Universities; Work; age effect; aqueous; base; career; chronic pain; clinical practice; corneal scar; experience; eye dryness; lacrimal; ocular surface; older patient; precursor cell; programs; research and development; skills; stem cell biology; symposium; translational clinical trial; vision science

DESCRIPTION (provided by applicant): This is a proposal for a K08 Mentored Clinical Scientist Career Development Award to train Dr. Vinay Kumar Aakalu. The primary goal of this application is to serve as a basis for training Dr. Aakalu in the critical skills necessary to deveop into an independent scientific investigator focused on lacrimal cell and precursor cell biology. The specific aims of the proposed educational program are the performance of 1) graduate level cell, molecular, stem cell biology and basic translational/clinical trial course work at the University of Illinois at Chicago 2) basic scientific investigations in accessory lacrimal cell and stem cell biology under the guidance of an experienced four person mentoring committee 3) seminar and conference participation at the University of Illinois and outside the University of Illinois. The mentoring committee is made up of experts in stem cell, ocular, molecular cell biology and translational research. Each mentor is well experienced in training clinician- scientists. The mentoring committee will be focused on guiding Dr. Aakalu in the development of research skills, analytical thinking and the ethical performance of basic and translational research. The overarching research goals of the proposal are to develop a better understanding of accessory lacrimal cell biology and to identify and isolate precursor cells from this tissue. Th specific aims of the project are to: 1. Develop a better understanding of ALG distribution, growth requirements, and changes with patient characteristics. 1a. A three dimensional map of the cadaveric human orbit will be constructed to determine the location of ALG tissue throughout the ocular surface as a basis for rational harvesting decisions. 1b. Characterize ALG tissue in human surgical specimens and relate the robustness or histopathologic changes in these tissues to relevant patient characteristics (age, gender, race, hormonal status). 1c. Develop ALG cultures from human surgical specimens to better understand requirements for cell survival and expansion. 2. Establish the presence of precursor cells in ALG tissue and isolate and characterize these precursor cells. 2a. Identify precursor marker positive ALG cells and determine a signature marker pattern using human specimens and immunohistochemistry on tissue microarrays. 2b. Isolate precursor candidate cells utilizing fluorescence cell sorting and determine if changes in precursor candidate cell populations are noted with different patient characteristics. 2c. Determine if precursor candidate cells isolated have stem cell properties. 2d. Establish cultures from precursor candidates and determine if these cultures can produce functional ALG cells.

描述（由申请人提供）：这是一份K 08指导临床科学家职业发展奖的提案，旨在培训Vinay Kumar Aakalu博士。该应用程序的主要目标是作为培训Aakalu博士所需的关键技能的基础，以发展成为专注于泪腺细胞和前体细胞生物学的独立科学研究者。拟议的教育计划的具体目标是1）研究生水平的细胞，分子，干细胞生物学和基本的转化/临床试验课程工作在伊利诺伊大学芝加哥2）在副泪腺细胞的基础科学研究和 在一个经验丰富的四人指导委员会的指导下进行干细胞生物学的研究。3）参加伊利诺伊大学和伊利诺伊大学以外的研讨会和会议。指导委员会由干细胞、眼科、分子细胞生物学和转化研究领域的专家组成。每位导师在培训临床医生-科学家方面经验丰富。指导委员会将专注于指导Aakalu博士发展研究技能，分析思维以及基础和转化研究的道德表现。该提案的总体研究目标是更好地了解副泪腺细胞生物学，并从该组织中识别和分离前体细胞。该项目的具体目标是：1。更好地了解ALG的分布、生长要求以及患者特征的变化。1a.将构建尸体人眼眶的三维图，以确定ALG组织在整个眼表中的位置，作为合理采集决策的基础。1b.表征人体手术标本中的ALG组织，并将这些组织中的稳健性或组织病理学变化与相关患者特征（年龄、性别、种族、激素状态）相关联。1c.从人类手术标本中开发ALG培养物，以更好地了解细胞存活和扩增的要求。2.建立ALG组织中前体细胞的存在，并分离和表征这些前体细胞。2a.鉴定前体标志物阳性ALG细胞，并使用人类标本和组织微阵列上的免疫组织化学确定特征标志物模式。2b.利用荧光细胞分选分离前体候选细胞，并确定前体候选细胞群的变化是否与不同的患者特征有关。2c.确定分离的前体候选细胞是否具有干细胞特性。2d. 从前体候选者建立培养物，并确定这些培养物是否可以产生功能性ALG细胞。