Study of Accessory Lacrimal Gland and Precursor Cell Biology

副泪腺及前体细胞生物学研究

• 批准号: 9044784
• 负责人: Vinay Aakalu
• 金额: $ 23.64万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2019-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9044784
• 关键词: ABCG2 gene; Address; Affect; Age; Anatomy; Atrophic; Basic Science; Biology; Blindness; Cell Separation; Cell Survival; Cells; Cellular biology; Characteristics; Chicago; Clinical; Clinical Investigator Award; Development; Disease; Ethics; Eye; Eye diseases; Film; Fluorescence; Gender; Gland; Goals; Growth; Harvest; Health; Histologic; Hormonal; Human; Illinois; Immunohistochemistry; Investigation; K-Series Research Career Programs; Knowledge; Lacrimal gland structure; Lead; Location; Lubrication; Maintenance; Maps; Mentors; Microscopic; Molecular; Mus; Ocular orbit; Operative Surgical Procedures; Patients; Pattern; Performance; Persons; Population; Production; Productivity; Property; Quality of life; Race; Regenerative Medicine; Research; Research Personnel; Scientist; Specimen; Stem cells; Surface; Techniques; Thinking; Tissue Microarray; Tissues; Training; Translational Research; Transplantation; Treatment Cost; Universities; Work; age effect; aqueous; base; career; chronic pain; clinical practice; corneal scar; experience; eye dryness; lacrimal; ocular surface; older patient; precursor cell; programs; research and development; skills; stem cell biology; symposium; translational clinical trial; vision science

DESCRIPTION (provided by applicant): This is a proposal for a K08 Mentored Clinical Scientist Career Development Award to train Dr. Vinay Kumar Aakalu. The primary goal of this application is to serve as a basis for training Dr. Aakalu in the critical skills necessary to deveop into an independent scientific investigator focused on lacrimal cell and precursor cell biology. The specific aims of the proposed educational program are the performance of 1) graduate level cell, molecular, stem cell biology and basic translational/clinical trial course work at the University of Illinois at Chicago 2) basic scientific investigations in accessory lacrimal cell and stem cell biology under the guidance of an experienced four person mentoring committee 3) seminar and conference participation at the University of Illinois and outside the University of Illinois. The mentoring committee is made up of experts in stem cell, ocular, molecular cell biology and translational research. Each mentor is well experienced in training clinician- scientists. The mentoring committee will be focused on guiding Dr. Aakalu in the development of research skills, analytical thinking and the ethical performance of basic and translational research. The overarching research goals of the proposal are to develop a better understanding of accessory lacrimal cell biology and to identify and isolate precursor cells from this tissue. Th specific aims of the project are to: 1. Develop a better understanding of ALG distribution, growth requirements, and changes with patient characteristics. 1a. A three dimensional map of the cadaveric human orbit will be constructed to determine the location of ALG tissue throughout the ocular surface as a basis for rational harvesting decisions. 1b. Characterize ALG tissue in human surgical specimens and relate the robustness or histopathologic changes in these tissues to relevant patient characteristics (age, gender, race, hormonal status). 1c. Develop ALG cultures from human surgical specimens to better understand requirements for cell survival and expansion. 2. Establish the presence of precursor cells in ALG tissue and isolate and characterize these precursor cells. 2a. Identify precursor marker positive ALG cells and determine a signature marker pattern using human specimens and immunohistochemistry on tissue microarrays. 2b. Isolate precursor candidate cells utilizing fluorescence cell sorting and determine if changes in precursor candidate cell populations are noted with different patient characteristics. 2c. Determine if precursor candidate cells isolated have stem cell properties. 2d. Establish cultures from precursor candidates and determine if these cultures can produce functional ALG cells.

描述（由申请人提供）：这是一份K08指导临床科学家职业发展奖的提案，旨在培训Vinay Kumar Aakalu博士。本申请的主要目的是为Aakalu博士提供必要的关键技能培训，使其成为一名专注于泪细胞和前体细胞生物学的独立科学研究者。该教育计划的具体目标是：1)伊利诺伊大学芝加哥分校研究生水平的细胞、分子、干细胞生物学和基础转化/临床试验课程；2)辅助泪细胞和泪细胞的基础科学研究