Cellular and Molecular Biology Core

项目概要：细胞和分子生物学核心 细胞和分子生物学核心为分子生物学、细胞生物学和分子生物学提供必要的设备和支持。 文化学组织学和病毒学重要的是，核心包含一些专门的工具， 要么对个体调查人员来说成本太高，必要但不经常使用，要么需要熟练的援助， 使用.其目标是提供设施，专业知识和服务：1）建立，扩大和维护 原代细胞、干细胞和转化的、转染的和可诱导的细胞系以及器官培养物， 用于视觉研究，2）进行眼科研究的细胞和分子生物学实验， 需要使用专门的仪器，3）辅助和协调涉及细胞培养的实验， 转染，病毒包装，引物设计，克隆，哺乳动物和病毒载体的构建，流动 流式细胞术，激光捕获显微切割，DNA测序，真实的时间PCR，DNA/microRNA微阵列， Luminex、蛋白质组学和蛋白质分析，以及4）协助组织的组织学和形态学研究 标本和培养细胞。

PROJECT SUMMARY: CELLULAR AND MOLECULAR BIOLOGY CORE The Cellular and Molecular Biology Core provides essential equipment and support for molecular biology, cell culture, histology, and virology. Importantly, the Core contains a number of specialized instruments that are either too costly for individual investigators, necessary but infrequently used, or require skilled assistance to use. The goals are to provide facilities, expertise, and services for: 1) establishing, expanding, and maintaining primary cells, stem cells, and transformed, transfected, and inducible cell lines as well as organ cultures for use in vision research, 2) performing cellular and molecular biology experiments for ophthalmic research that require use of specialized instrumentation, 3) assisting and coordinating experiments that involve cell culturing, transfection, viral packaging, primer design, cloning, construction of mammalian and viral vectors, flow cytometry, laser capture microdissection, DNA sequencing, real time PCR, DNA/microRNA microarray, Luminex, proteomics, and protein analyses, and 4) assisting in histologic and morphological studies of tissue specimens and cultured cells.