BONE MORPHOMETRY AND BIOMECHANICS CORE

骨形态学和生物力学核心

• 批准号: 8663770
• 负责人: ROBERT Stewart WEINSTEIN
• 金额: $ 29.71万
• 依托单位: UNIV OF ARKANSAS FOR MED SCIS
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8663770
• 关键词: 3-Dimensional; Age; Aging; American; Animals; Apoptosis; Architecture; Area; Autopsy; Biomechanics; Blood Circulation; Blood Vessels; Calibration; Cell Count; Cells; Continuing Education; Cryoultramicrotomy; Diet; Doxycycline; Equipment; Femur; Fluorescence; Fracture; Frozen Sections; Galactosidase; Generic Drugs; Green Fluorescent Proteins; Human Resources; Ibuprofen; In Situ Nick-End Labeling; Individual; Infiltration; Infusion procedures; Injection of therapeutic agent; Institutes; Instruction; Investigation; Knowledge; Label; Laboratories; LacZ Genes; Life; Lighting; Materials Testing; Measurement; Measures; Methylmethacrylate; Microtomy; Modeling; Molecular; Mus; Osteoblasts; Osteocytes; Osteoporosis; Paraffin Embedding; Perfusion; Peripheral; Persons; Plastic Embedding; Prevalence; Procedures; Property; Reading; Running; Silicon; Societies; Specimen; Spinal; Staining method; Stains; Steel; Structure; Surface; System; Tablets; Tail; Technology; Tetracyclines; Time; Training; Transgenic Animals; Vascular System; Veins; Work; X-Ray Computed Tomography; bone; bone cell; bone mass; bone strength; bone toughness; cost; cryostat; density; lead chromate; lumbar vertebra bone structure; morphometry; multidisciplinary; programs; promoter; research study; solute; spine bone structure

The purpose of the Bone Morphometry and Biomechanics Core is to consolidate key personnel and equipment in order to provide a centralized facility that will enhance collaborative and multidisciplinary investigations into the multiple mechanisms that increase bone fragility with age. Core C will perform six procedures in a cost and time efficient manner to facilitate the examination of the changes in bone cell number, architecture, mass, vascularity, and strength in murine models. 1. Undecalcified, plastic embedded, tetracycline labeled sections of vertebral cortical and cancellous bone and femoral diaphyseal cortical bone will be prepared and read histomorphometrically including measurements of the prevalence of osteoblast and osteocyte apoptosis, solute transport from the peripheral circulation to the lacunar-canalicular system as revealed by procion red epifluorescence, and proximity of vascular channels to the tetracycline labeled cancellous perimeter as revealed by the infusion of lead chromate. 2. Frozen sections of bone will be obtained to facilitate quantification of galactosidase activity, immunostaining of targeted cells in transgenic animals and recognition of green fluorescent protein. 3. Micro-CT measurements will be done at necropsy in excised lumbar vertebrae and femora to determine the individual volumetric densitometric contributions of cortical and cancellous bone as well as the 3-dimensional measurements of cortical and cancellous microarchitecture. In addition, the volume and suri'ace area of the vascular system will be measured usingmicro-CT imaging of decalcified bones after perfusion with lead chromate. 4. Bone mass will be determined by dual-energy x-ray absorptiometry. Measurements of the integral density of the global, spinal, and femoral subregions will be performed serially, in longitudinal experiments in live mice. 5. Vertebral compression strength and femoral three-point bending will be measured to determine the significance of the changes in BMD and bone architecture. 6. The Core will train and maintain the continuing education of a key laboratory person from each project to help perform the routine static and dynamic bone histomorphometry.

骨形态测量和生物力学核心的目的是整合关键人员和设备，以提供一个集中设施，加强对随着年龄增长而增加骨骼脆性的多种机制的协作和多学科研究。 Core C 将以成本和时间高效的方式执行六个程序，以方便检查小鼠模型中骨细胞数量、结构、质量、血管分布和强度的变化。 1. 未脱钙、塑性 将制备嵌入的、四环素标记的椎骨皮质和松质骨以及股骨骨干皮质骨切片，并通过组织形态计量学读取，包括测量成骨细胞和骨细胞凋亡的发生率、普罗翁红落射荧光显示的溶质从外周循环到腔隙-小管系统的转运， 以及通过注入铬酸铅显示血管通道与四环素标记的松质周界的接近程度。 2.获得骨的冰冻切片，以便于半乳糖苷酶活性的定量、转基因动物中靶细胞的免疫染色以及绿色荧光蛋白的识别。 3. 尸检时将进行显微 CT 测量 在切除的腰椎和股骨中，以确定皮质和松质骨的个体体积密度贡献以及皮质和松质骨微结构的 3 维测量。此外，在灌注铬酸铅后，将使用脱钙骨的显微 CT 成像来测量血管系统的体积和表面面积。 4. 骨量采用双能X射线吸收法测定。将在活体小鼠的纵向实验中连续进行整体、脊柱和股骨分区的整体密度测量。 5. 测量椎骨压缩强度和股骨三点弯曲，以确定 BMD 和骨结构变化的意义。 6. 核心将培训和维持每个项目的关键实验室人员的继续教育，以帮助进行常规静态和动态骨组织形态测定。