Project 1 - Salk Institute for Biological Studies NINDS Center Core Grant

个别NINDS受赠者努力雇用和发展尖端的 基因工具来操纵小鼠基因组，以测试他们在NINDS资助的 研究项目。产生这些细胞系中的许多需要多个步骤来产生小鼠ES细胞， 这些线通常是费力的或难以获得的。产生这样的ES线的成本和时间是主要问题。 成功的动物模型开发的瓶颈，因为它阻止了许多NINDS资助的研究人员， 产生基因工程小鼠，即使是对于那些精通所需的先进和非常 具体技术。一个中央基因组修改核心将提供服务，打破障碍， 通过支持设计ES细胞系的生产来产生遗传修饰的小鼠系。集中 通用汽车核心将提高效率，降低生产这些线路的成本。此外，中央服务将 为协同开发对所有用户群体都有价值的产品线提供机会。的 研究所承诺为建立全球机制核心提供空间， 满足神经科学中心研究人员不断变化的需求。通用汽车核心的主要目标是提供 在胚胎干细胞中操作小鼠基因组的服务，这对于研究 神经功能、结构和发育以及体外和体内小鼠模型的产生 疾病。

Individual NINDS grantees have strived to employ and develop cutting-edge genetic tools to manipulate the mouse genome to test specific hypotheses proposed in their NINDS-funded research projects. Producing many of these lines has required multiple steps in generating mouse ES cells and these lines are often laborious or difficult to obtain. The cost and time for generating such ES lines is a major bottleneck for successful animal model development as it prohibits many NINDS funded investigators from generating genetically-engineering mice, even for labs that are versed in the required advanced and very specific technologies. A central genome modification core will provide services to break the barriers to generate genetically modified mouse lines by supporting the production of designer ES-cell lines. A centralized GM Core will increase efficiency and lower the cost of generating such lines. In addition, a central service will provide opportunities for synergistic development of lines that will be of value across all user groups. The Institute has made a commitment to provide space for establishment of the GM core that would lead the way to meet the evolving needs of Neuroscience Center investigators. The major goal of the GM Core is to provide services for manipulation ofthe mouse genome in ES cells that are essential for studies ofthe mechanisms of neural function, structure and development as well as the generation of both in vitro and in vivo mouse models of diseases.