Project 1 - Salk Institute for Biological Studies NINDS Center Core Grant

NINDS个人受赠者努力雇用和发展尖端技术 用于操纵小鼠基因组以检验其NINDS资助的特定假说的遗传工具 研究项目。生产许多这样的品系需要多个步骤来生成小鼠胚胎干细胞和 这些线路往往很费力，或者很难获得。生成这样的ES线路的成本和时间是主要的 动物模型成功开发的瓶颈，因为它禁止许多NINDS资助的研究人员 产生基因工程小鼠，即使是对于精通所需的先进和非常 具体的技术。一个中央基因组修改核心将提供服务，以打破障碍 通过支持设计的胚胎干细胞系的生产来产生转基因小鼠系。一个集中化的 GM Core将提高效率并降低生产此类生产线的成本。此外，还将设立一个中央服务处 为协同开发对所有用户群体都有价值的产品线提供机会。这个 研究所承诺为建立全球机制核心提供空间，这将导致 满足神经科学中心研究人员不断发展的需求。通用汽车核心的主要目标是提供 在ES细胞中操纵小鼠基因组的服务，这对于研究EST的机制是必不可少的 神经功能、结构和发育以及体外和体内小鼠模型的产生 疾病的威胁。

Individual NINDS grantees have strived to employ and develop cutting-edge genetic tools to manipulate the mouse genome to test specific hypotheses proposed in their NINDS-funded research projects. Producing many of these lines has required multiple steps in generating mouse ES cells and these lines are often laborious or difficult to obtain. The cost and time for generating such ES lines is a major bottleneck for successful animal model development as it prohibits many NINDS funded investigators from generating genetically-engineering mice, even for labs that are versed in the required advanced and very specific technologies. A central genome modification core will provide services to break the barriers to generate genetically modified mouse lines by supporting the production of designer ES-cell lines. A centralized GM Core will increase efficiency and lower the cost of generating such lines. In addition, a central service will provide opportunities for synergistic development of lines that will be of value across all user groups. The Institute has made a commitment to provide space for establishment of the GM core that would lead the way to meet the evolving needs of Neuroscience Center investigators. The major goal of the GM Core is to provide services for manipulation ofthe mouse genome in ES cells that are essential for studies ofthe mechanisms of neural function, structure and development as well as the generation of both in vitro and in vivo mouse models of diseases.