Multi-region, extended-depth imaging of neural activity via a novel needle microendoscope

通过新型针显微内窥镜对神经活动进行多区域、深度成像

• 批准号: 9093803
• 负责人: Jerome Mertz
• 金额: $ 20.46万
• 依托单位: BOSTON UNIVERSITY (CHARLES RIVER CAMPUS)
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-01 至 2018-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9093803
• 关键词: Animals; Area; BRAIN initiative; Brain region; Calcium; Caliber; Cells; Cortical Column; Coupling; Data; Development; Electrodes; Feedback; Fiber; Fluorescence; Health; Image; Lighting; Measurement; Microscopy; Miniaturization; Mus; Needles; Neocortex; Optics; Penetration; Play; Process; Resolution; Role; Scanning; Source; Structure; System; Tactile; Techniques; Technology; Thalamic structure; Tissues; Vibrissae; awake; base; brain tissue; calcium indicator; cell type; design; detector; improved; in vivo imaging; innovation; interest; lens; light scattering; novel; optogenetics; relating to nervous system; research study; sensor; somatosensory; tool; virtual

 DESCRIPTION (provided by applicant): With the continuing expansion of optogenetic tools, the central importance of optical measurement of neural activity has and will continue to grow. However, large scale optical approaches, such as pursued by the BRAIN Initiative, must overcome high light scattering in brain tissue. Moreover, optical approaches should be feasible in behaving animals, including freely moving mice. We will develop an ultra-miniature microendoscope, termed a needle optrode, made with a bare fiber bundle beveled to a fine tip. The smaller size and beveling improves tissue penetration and lowers tissue damage compared to existing technology, and arranges the field of view for imaging across layered structures such as neocortex. The core project contribution is achieving miniaturization through a lensless design, using an innovative coupling of array detector to enable fluorescence background rejection and remote focusing. We will demonstrate the power of our approach for collecting large scale, multiregion data by recording simultaneously from aligned cortical and thalamic regions of the mouse somatosensory system, in anesthetized mice. Completion of these aims will prepare us to record simultaneously throughout an entire thalamocortical whisker processing network, including both feedforward and feedback projections, and be a first step towards performing these recordings in an awake animal actively engaging objects of interest in a tactile task. Moreover, our approach --- providing multiregion, cellular resolution recording of genetically identified cell types, with possible extension to behaving animals --- will support similar experiments across brain regions and systems, as one of the high priority components of the BRAIN initiative.



项目成果

Dual-modality endomicroscopy with co-registered fluorescence and phase contrast.

具有共同配准荧光和相差的双模态内窥镜检查。

• DOI: 10.1364/boe.7.003403
• 发表时间: 2016
• 期刊: Biomedical optics express
• 影响因子: 3.4
• 作者: Ba,C;Palmiere,M;Ritt,J;Mertz,J
• 通讯作者: Mertz,J