IGF::OT::IGF A MULTIFUNCTIONAL, REUSABLE AND AUTOMATED MICROFLUIDIC SMART PLATE FOR HIGH THROUGHPUT LIVE CELL ASSAYS

IGF::OT::IGF 用于高通量活细胞检测的多功能、可重复使用的自动化微流控智能板

• 批准号: 9355754
• 负责人: MARY LUDLAM
• 金额: $ 23万
• 依托单位: CAIRN BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-15 至 2017-02-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9355754
• 关键词: Biological Assay; Cells; Devices; Electronics; Environmental Risk Factor; Foundations; Image; Industry; Life; Manufacturer Name; Measurement; Measures; Methods; Microfluidics; Microscope; Monitor; Research; Research Personnel; Stimulus; System; Testing; Time; cost; design; interest; prototype; research study; response; sensor

This project aims to design and develop a multifunctional, reusable and automated microfluidic smart-plate for high throughput, live cell assays. This system will combine a microfluidic multi-well chamber array and an environmental measurement system into a single device, enabling high accuracy and closed-loop control over experiment environmental factors. Existing environmental control chambers are large, physically cumbersome and expensive to obtain, while being designed for a single microscope type and configuration. Our device is a singular solution for live cell time-lapse and environmental response studies. With a single device, researchers can inject stimuli, monitor and alter environmental factors, capture high numerical aperture images, and measure confluence. The device will make use of commonly available components and manufacturing methods, enabling replication by a wide range of interested parties, from research labs to industry manufacturers.

本项目旨在设计和开发一种多功能、可重复使用和自动化的微流控智能板，用于高通量活细胞检测。该系统将联合收割机与微流控多孔腔阵列和环境测量系统集成到一个装置中，实现了对实验环境因素的高精度和闭环控制。现有的环境控制室是大的，物理上笨重和昂贵的获得，而被设计为一个单一的显微镜类型和配置。我们的设备是活细胞延时和环境响应研究的独特解决方案。通过一个单一的设备，研究人员可以注入刺激，监测和改变环境因素，捕获高数值孔径图像，并测量汇流。该设备将利用常用的组件和制造方法，使从研究实验室到工业制造商的广泛利益相关方能够复制。