Optimizing Nanopore Protein Interactions

优化纳米孔蛋白质相互作用

• 批准号: 8892399
• 负责人: Eric Ervin
• 金额: $ 23.5万
• 依托单位: ELECTRONIC BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-04-15 至 2017-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8892399
• 关键词: Address; Amino Acid Sequence; Amino Acids; Automobile Driving; Biological Markers; Characteristics; Chemicals; Complex; Data Analyses; Databases; Detection; Development; Diagnosis; Discrimination; Disease; Electronics; Evaluation; Foundations; Gene Expression; Genomics; Goals; Government; Immunoassay; Individual; Lead; Mass Spectrum Analysis; Measurement; Methodology; Methods; Monitor; Peptide Sequence Determination; Peptides; Persons; Phosphorylation; Pore Proteins; Post-Translational Protein Processing; Preparation; Property; Protein translocation; Proteins; Proteomics; Research Personnel; Sampling; Sequence Determination; System; Techniques; Temperature; Testing; Time; Validation; Variant; Work; X-Ray Crystallography; base; constriction; cost; driving force; electric field; glycosylation; instrumentation; mutant; nanopore; pathogen; pressure; programs; public health relevance; research clinical testing; tool; voltage

 DESCRIPTION (provided by applicant): Electronic BioSciences (EBS) will begin examining the characteristics and interactions that will eventually enable nanopore based differentiation of peptides and amino acids. Although there are numerous components of a complete nanopore based protein characterization system that will ultimately need to be developed, during the course of this two year program EBS will focus solely on the detection and differentiation methodologies in order to demonstrate basic feasibility and proof-of-concept. The information generated from these studies will provide the foundation for developing the nanopore based protein characterization concept and eventually will lead to the development of cutting edge proteomics instrumentation.

 描述（由申请人提供）：电子生物科学（EBS）将开始研究特性和相互作用，最终实现基于纳米孔的肽和氨基酸的区分。尽管最终需要开发基于纳米孔的完整蛋白质表征系统的许多组成部分，但在这两年的计划过程中，EBS 将仅专注于检测和区分方法，以证明基本的可行性和概念验证。这些研究产生的信息将为开发基于纳米孔的蛋白质表征概念提供基础，并最终导致尖端蛋白质组学仪器的开发。