Nanopore Enabled Exonuclease Sequencing

纳米孔核酸外切酶测序

• 批准号: 9171771
• 负责人: Eric Ervin
• 金额: $ 22.5万
• 依托单位: ELECTRONIC BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-16 至 2018-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9171771
• 关键词: Address; Base Sequence; Benchmarking; Binding; Biological; Chemicals; Cyclodextrins; DNA; DNA Sequence; DNA Sequence Alteration; Detection; Development; Devices; Discrimination; Engineering; Evaluation; Exonuclease; Film; Geometry; Glass; Goals; Government; Health; Hereditary Disease; Human; Individual; Lipid Bilayers; Measurement; Membrane; Methodology; Methods; Molecular; Nature; Nucleotides; Oral cavity; Pathogen detection; Persons; Phase; Polymers; Pore Proteins; Property; Reading; Reproducibility; Shapes; Silicon; Small Business Innovation Research Grant; Solid; Specificity; System; Temperature; Transport Process; base; cost; fluid flow; genome sequencing; interest; nanopore; novel; personalized medicine; pressure; prevent; programs; research study; sensor; success; voltage

Project Summary The overall goal of this program is to develop a nanopore that will allow DNA mononucleotides, such as those released from an exonuclease, to be captured and read with 100% accuracy, thus enabling the exonuclease/nanopore-based sequencing concept. While base discrimination with a molecular adapter has been previously demonstrated, capturing the molecules with 100% efficiency is the current limitation. By creating the proposed device, we will be able to use novel methods to control the molecular transport rate of the mononucleotides to and through the pore, enabling 100% capture efficiency.

项目摘要 该计划的总体目标是开发一种纳米孔，使DNA单核苷酸，如那些 从核酸外切酶释放，可以100%准确地捕获和读取，从而使 基于核酸外切酶/纳米孔的测序概念。而用分子适配器进行碱基识别则具有 以前已经证明，以100%的效率捕获分子是目前的限制。通过 发明了这个装置，我们将能够使用新的方法来控制分子传输速率 单核苷酸进入和通过毛孔，实现100%的捕获效率。