Nanopore Enabled Exonuclease Sequencing
纳米孔核酸外切酶测序
基本信息
- 批准号:9171771
- 负责人:
- 金额:$ 22.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-09-16 至 2018-02-28
- 项目状态:已结题
- 来源:
- 关键词:AddressBase SequenceBenchmarkingBindingBiologicalChemicalsCyclodextrinsDNADNA SequenceDNA Sequence AlterationDetectionDevelopmentDevicesDiscriminationEngineeringEvaluationExonucleaseFilmGeometryGlassGoalsGovernmentHealthHereditary DiseaseHumanIndividualLipid BilayersMeasurementMembraneMethodologyMethodsMolecularNatureNucleotidesOral cavityPathogen detectionPersonsPhasePolymersPore ProteinsPropertyReadingReproducibilityShapesSiliconSmall Business Innovation Research GrantSolidSpecificitySystemTemperatureTransport Processbasecostfluid flowgenome sequencinginterestnanoporenovelpersonalized medicinepressurepreventprogramsresearch studysensorsuccessvoltage
项目摘要
Project Summary
The overall goal of this program is to develop a nanopore that will allow DNA mononucleotides, such as those
released from an exonuclease, to be captured and read with 100% accuracy, thus enabling the
exonuclease/nanopore-based sequencing concept. While base discrimination with a molecular adapter has
been previously demonstrated, capturing the molecules with 100% efficiency is the current limitation. By
creating the proposed device, we will be able to use novel methods to control the molecular transport rate of
the mononucleotides to and through the pore, enabling 100% capture efficiency.
项目摘要
该计划的总体目标是开发一种纳米孔,使DNA单核苷酸,如那些
从核酸外切酶释放,可以100%准确地捕获和读取,从而使
基于核酸外切酶/纳米孔的测序概念。而用分子适配器进行碱基识别则具有
以前已经证明,以100%的效率捕获分子是目前的限制。通过
发明了这个装置,我们将能够使用新的方法来控制分子传输速率
单核苷酸进入和通过毛孔,实现100%的捕获效率。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Eric Ervin其他文献
Eric Ervin的其他文献
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{{ truncateString('Eric Ervin', 18)}}的其他基金
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- 批准号:
10322603 - 财政年份:2021
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$ 22.5万 - 项目类别:
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