Identification and Quantification of Sequence Specific RNA Modifications via the Alpha-Hemolysin Latch Zone

通过 α-溶血素锁存区对序列特异性 RNA 修饰进行鉴定和定量

• 批准号: 8841921
• 负责人: Eric Ervin
• 金额: $ 21.57万
• 依托单位: ELECTRONIC BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2016-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8841921
• 关键词: Base Pairing; Binding; Biological; Caliber; Chemicals; Chemistry; Collaborations; Detection; Development; Diagnostic; Disease; Electronics; Engineering; Epigenetic Process; Face; Gene Expression Regulation; Genomics; Goals; Health; Hemolysin; Laboratories; Left; Lesion; Major Groove; Messenger RNA; Metabolism; Methodology; Methods; Modification; Nucleotides; Peptide Nucleic Acids; Phase; Positioning Attribute; RNA; RNA Caps; Regulation; Relative (related person); Research; Role; Sampling; Series; Signal Transduction; Site; Site-Directed Mutagenesis; Small Business Technology Transfer Research; Structure; System; Tail; Technology; Time; Universities; Utah; base; cost; design; improved; interest; nanopore; novel; programs; triple helix

DESCRIPTION (provided by applicant): During this STTR program, Electronic BioSciences (EBS) proposes to develop a nanopore based system around our University of Utah collaborators newly developed technology that will be capable of identifying and quantifying sequence specific RNA modifications. A system capable of quantitative, low cost, high throughput, RNA modification characterization has the capability to elucidate the roles of these modifications in cellular signaling, and improve our understanding of gene regulation and associated disease states. The goal of this Phase I program is to demonstrate proof-of-concept for the RNA modification characterization concept, and determine the parameters that will allow our team to develop a complete RNA modification characterization system during a follow-on Phase II program.

描述（由申请人提供）：在此STTR计划期间，电子生物科学（EBS）提出围绕我们的犹他州大学合作者新开发的技术开发基于纳米孔的系统，该技术将能够识别和定量序列特异性RNA修饰。能够定量、低成本、高通量、RNA修饰表征的系统具有阐明这些修饰在细胞信号传导中的作用的能力，并提高我们对基因调控和相关疾病状态的理解。这个I期项目的目标是证明RNA修饰表征概念的概念验证，并确定允许我们的团队在后续II期项目中开发完整的RNA修饰表征系统的参数。