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Spatiotemporal Control of Dynamic Notch Signaling with Subcellular Resolution

具有亚细胞分辨率的动态Notch信号传导的时空控制

基本信息

批准号：
9122436
负责人：
Young-wook Jun
金额：
$ 30.12万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-08-01 至 2019-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9122436
关键词：
Address Atomic Force Microscopy Behavior Biological Process Calibration Cell Communication Cell Line Cell Nucleus Cell surface Cells Chemistry Critical Pathways Development Diagnostic Dimensions Disease Elements Endocytosis Event Exertion Genes Goals Hand Health Human Image Imagery In Situ In Vitro Individual Intracellular translocation Investigation Knowledge Label Life Ligands Location Lymphoma Magnetism Maintenance Malignant Neoplasms Measures Mechanics Mediating Methods Modeling Molecular Monitor Multiple Sclerosis Nanotechnology Normal tissue morphology Nucleic Acid Regulatory Sequences Oligonucleotides Optics Play Population Process Property Proteins Proteolysis Receptor Activation Receptor Signaling Regulation Resolution Role Signal Transduction Spatial Distribution Stem cells System Techniques Technology Therapeutic Time angiogenesis cell behavior design innovation magnetic field nanoparticle nanoprobe nanosystems nerve stem cell neurodevelopment next generation notch protein plasmonics receptor research study signal processing single molecule spatiotemporal stem cell therapy tool

项目摘要

DESCRIPTION (provided by applicant): Notch signaling is a highly conserved cell-to-cell communication mechanism, which plays a central role in defining individual cells' behaviors and fates during development. Despite rapidly increasing knowledge of these signaling events, little is known about how spatiotemporal dynamics of receptor signaling across the cell influence signal exchange. To address this challenge, we propose an advanced nanosystem that mimics, enabling simultaneous real-time monitoring and in situ regulation of Notch signaling in a particular cell at any desired location and time with subcellular resolution. Using this new nanotechnology, we first determine force-induced structural features of Notch receptors. We also explore Notch signaling in neural stem cells to determine how the spatiotemporal distribution of Notch signaling across a cell population influences the final fate of individual cels during development. Binary cell communication via Notch.

描述（由申请人提供）：Notch信号传导是一种高度保守的细胞间通讯机制，在发育过程中定义单个细胞的行为和命运中起着核心作用。尽管对这些信号事件的了解迅速增加，但对跨细胞的受体信号传导的时空动态如何影响信号交换知之甚少。为了应对这一挑战，我们提出了一种先进的纳米系统，该系统可以模拟，在任何所需的位置和时间，以亚细胞分辨率同时实时监测和原位调节特定细胞中的Notch信号传导。使用这种新的纳米技术，我们首先确定Notch受体的力诱导结构特征。我们还探讨了神经干细胞中的Notch信号传导，以确定Notch信号在细胞群中的时空分布如何影响发育过程中个体神经元的最终命运。通过Notch进行二进制单元通信。