权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Mouse Genetics Core

小鼠遗传学核心

基本信息

批准号：
9151169
负责人：
EBENEZER N YAMOAH
金额：
$ 26.22万
依托单位：
UNIVERSITY OF NEVADA RENO
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-01 至 2021-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9151169
关键词：
Address Age Aging Animal Model Auditory Auditory system Backcrossings Blinded Breeding Code Data Development Disasters Disease Outbreaks Doxycycline Endolymph Ensure Epithelium Facility Controls Gene Targeting Generations Genes Genetic Genetically Engineered Mouse Genetically Modified Animals Genotype Goals Hair Cells Human Resources In Vitro Individual Laboratories Labyrinth Mediating Modeling Molecular Mus Mutant Strains Mice Nerve Degeneration Neural Pathways Neurons Outcome Perilymph Presbycusis Process Program Research Project Grants Proteins Research Resources Rodent Role Sensory Sensory Hair Services Site Sum Synapses Testing Tetanus Helper Peptide Therapeutic Tight Junctions Transcript Transgenic Mice Transgenic Model United States National Institutes of Health abstracting age related aged aging gene animal facility combat cost effective design genetic approach handbook hearing impairment in vivo innovation interest meetings mouse model mutant novel occludin optogenetics programs relating to nervous system research study senescence spiral ganglion success

项目摘要

Abstract Development and utilization of aged and genetically engineered mouse models are crucial to understanding the roles of specific genes in age-related hearing loss (ARHL). We will require strain-specific aged (senescent) mice that are not available commercially and are produced at the NIA-supported facility at the NIH, http://www.nia.nih.gov/research/dab/aged-rodent-colonies-handbook. All projects and Core C within the proposed PPG makes extensive use of these aged mice, and utilize a variety of genetic approaches to study the role of several genes in the inner ear and auditory neural pathways. Indeed, the proposed PPG will require the use of diseased models that result in hearing loss in the aging auditory system. The purpose of the mouse core is to provide services for the establishment of a strain-specific aged mouse colony and the generation and management of gene-targeted and transgenic mice. Founder mice will be identified, bred, and maintained in an animal facility at UNR. Additionally, several mouse lines will be bred and aged and supplied for experiments proposed in all three projects and Core C. The Core will also be responsible for maintaining and distributing mouse lines to each individual project. This will provide the most cost effective sharing and utilization of the mouse models. In addition, the Core will also cryopreserve critical lines in case of an inadvertent loss. Finally, the Core will be a resource for other laboratories interested in mouse models of ARHL.

摘要