Picosecond pulse technology for non-invasive electrostimulation
用于无创电刺激的皮秒脉冲技术
基本信息
- 批准号:8811947
- 负责人:
- 金额:$ 18.26万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2014
- 资助国家:美国
- 起止时间:2014-03-01 至 2017-02-28
- 项目状态:已结题
- 来源:
- 关键词:3-DimensionalAction PotentialsAddressAdverse effectsApoptoticAreaBiologicalBrainCell membraneCellsComputer SimulationDeep Brain StimulationDependencyDevelopmentDiseaseDoseDyesDystoniaElectric StimulationElectrodesElectromagnetic EnergyEndoplasmic ReticulumEngineeringFeasibility StudiesFura-2HeadHealthHeartHumanHuman bodyImageImplantIn VitroIndividualIntractable PainLifeMeasurementMeasuresMedicalMembraneMental disordersMethodsMicroscopeMicroscopyModelingMonitorNecrosisNeuromuscular DiseasesNeuronsPainParkinson DiseasePathway interactionsPenetrationPhysiologic pulseProcessResolutionSafetySignal TransductionSpottingsStimulusStructureSystemTechnologyTestingTimeTissuesTrainingTranscranial magnetic stimulationbasecell injurycellular imagingelectric fieldimprovedin vivolensmillisecondmodels and simulationnanosecondnovel strategiespatch clampprototyperatiometricrelating to nervous systemresponsesimulationtime intervalvoltage
项目摘要
DESCRIPTION (provided by applicant): Electric stimulation of cells and tissues is the basis of diverse medical treatments. However, stimulation of deep structures is usually invasive and relies on electrodes that are inserted or permanently implanted into the body. Transcranial magnetic stimulation (TMS) is an example of a non-invasive technology, but its penetration depth and precision are limited. Thus far, deep-penetrating but non-invasive electrostimulation has not been possible. However, recent developments in picosecond pulse technology offer an opportunity to overcome physical limitations and to deliver electric stimuli deep into the human body without using electrodes. We propose to employ intense picosecond-duration electric pulses (psEP) as a substitute for conventional electric stimulation with longer (micro- and millisecond) pulses. Instead of electrodes, psEP can be delivered by ultrawideband antennas in the form of electromagnetic waves, and focused at a depth in the human body without insertion of electrodes. Computer simulations predict significantly deeper penetration and better focusing of 200-ps pulses in comparison with TMS. Also, we have assembled and tested a prototype of an in vitro psEP exposure system and, for the first time, were able to demonstrate that 200-ps EP can indeed evoke action potentials in cultured neurons. This interdisciplinary project combines an engineering effort to develop and characterize psEP exposure systems with biological analyses of the efficiency and safety of electrostimulation by psEP. Specifically, this
project consists of five Aims intended to 1) develop a microscopy- and patch clamp- compatible system for psEP studies in vitro, 2) perform high-resolution computer simulations of psEP delivery in a realistic human head model, 3) quantify electrostimulation parameters in vitro for single pulses and trains of 200-ps pulses, 4) analyze Ca2+ dynamics in psEP-treated excitable and non-excitable cells, and 5) determine the safety margin between stimulatory effects and cell damage. This study will provide guidance for engineering of a high voltage picosecond pulser and antenna for deep-brain stimulation. It will also lay the ground for first in vivo trials of no-invasive psEP electrostimulation.
描述(申请人提供):细胞和组织的电刺激是各种医疗治疗的基础。然而,对深层结构的刺激通常是侵入性的,依赖于插入或永久植入体内的电极。经颅磁刺激(TMS)是一种非侵入性技术,但其穿透深度和精度有限。到目前为止,深度穿透但非侵入性的电刺激还不可能。然而,皮秒脉冲技术的最新发展提供了一个机会,可以克服物理限制,在不使用电极的情况下将电刺激深入人体。我们建议使用高强度皮秒持续时间的电脉冲(PsEP)来代替传统的具有更长(微秒和毫秒)脉冲的电刺激。代替电极,PSEP可以通过超宽带天线以电磁波的形式传递,并在不插入电极的情况下聚焦在人体的某一深度。计算机模拟预测,与TMS相比,200-ps脉冲具有更深的穿透深度和更好的聚焦能力。此外,我们还组装并测试了一个体外psEP暴露系统的原型,并首次能够证明200-ps EP确实可以在培养的神经元中诱发动作电位。这个跨学科的项目结合了开发和表征psEP暴露系统的工程努力,以及对psEP电刺激的效率和安全性的生物学分析。具体地说,这
该项目有五个目标,旨在1)开发一种与显微镜和膜片钳兼容的系统用于psEP的体外研究,2)在真实的人头模型中对psEP的释放进行高分辨率的计算机模拟,3)对单个脉冲和200 ps脉冲序列的体外电刺激参数进行量化,4)分析经psEP处理的可兴奋和不可兴奋细胞中的钙动力学,以及5)确定刺激效应和细胞损伤之间的安全界限。这项研究将为深部脑刺激高压皮秒脉冲发生器和天线的工程设计提供指导。这也将为首次无创的psEP电刺激体内试验奠定基础。
项目成果
期刊论文数量(6)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
A subnanosecond electric pulse exposure system for biological cells.
- DOI:10.1007/s11517-016-1516-7
- 发表时间:2017-07
- 期刊:
- 影响因子:3.2
- 作者:Xiao S;Semenov I;Petrella R;Pakhomov AG;Schoenbach KH
- 通讯作者:Schoenbach KH
3D bioprinter applied picosecond pulsed electric fields for targeted manipulation of proliferation and lineage specific gene expression in neural stem cells.
- DOI:10.1088/1741-2552/aac8ec
- 发表时间:2018-10
- 期刊:
- 影响因子:4
- 作者:Petrella RA;Mollica PA;Zamponi M;Reid JA;Xiao S;Bruno RD;Sachs PC
- 通讯作者:Sachs PC
A Dielectric Rod Antenna for Picosecond Pulse Stimulation of Neurological Tissue.
- DOI:10.1109/tps.2016.2537213
- 发表时间:2016-04
- 期刊:
- 影响因子:0
- 作者:Petrella RA;Schoenbach KH;Xiao S
- 通讯作者:Xiao S
Picosecond and Terahertz Perturbation of Interfacial Water and Electropermeabilization of Biological Membranes.
- DOI:10.1007/s00232-015-9788-7
- 发表时间:2015-10
- 期刊:
- 影响因子:0
- 作者:Vernier PT;Levine ZA;Ho MC;Xiao S;Semenov I;Pakhomov AG
- 通讯作者:Pakhomov AG
Electroporation by subnanosecond pulses.
- DOI:10.1016/j.bbrep.2016.05.002
- 发表时间:2016-07
- 期刊:
- 影响因子:2.7
- 作者:Semenov I;Xiao S;Pakhomov AG
- 通讯作者:Pakhomov AG
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Andrei G Pakhomov其他文献
Andrei G Pakhomov的其他文献
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{{ truncateString('Andrei G Pakhomov', 18)}}的其他基金
Next Generation Temporal Interference Stimulation for Non-Invasive Neuromodulation
用于非侵入性神经调节的下一代时间干扰刺激
- 批准号:
10615485 - 财政年份:2023
- 资助金额:
$ 18.26万 - 项目类别:
Targeted Neuromodulation by Nanosecond Pulsed Electric Fields
纳秒脉冲电场的靶向神经调节
- 批准号:
10669767 - 财政年份:2022
- 资助金额:
$ 18.26万 - 项目类别:
Targeted Neuromodulation by Nanosecond Pulsed Electric Fields
纳秒脉冲电场的靶向神经调节
- 批准号:
10515459 - 财政年份:2022
- 资助金额:
$ 18.26万 - 项目类别:
Low Energy Defibrillation with Nanosecond Pulsed Electric Field
纳秒脉冲电场低能量除颤
- 批准号:
8941895 - 财政年份:2015
- 资助金额:
$ 18.26万 - 项目类别:
Low Energy Defibrillation with Nanosecond Pulsed Electric Field
纳秒脉冲电场低能量除颤
- 批准号:
9278268 - 财政年份:2015
- 资助金额:
$ 18.26万 - 项目类别:
Picosecond pulse technology for non-invasive electrostimulation
用于无创电刺激的皮秒脉冲技术
- 批准号:
8636788 - 财政年份:2014
- 资助金额:
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Mechanisms and Implications of Nanoelectroporation in Living Cells
活细胞纳米电穿孔的机制和意义
- 批准号:
8099680 - 财政年份:2010
- 资助金额:
$ 18.26万 - 项目类别:
Mechanisms and Implications of Nanoelectroporation in Living Cells
活细胞纳米电穿孔的机制和意义
- 批准号:
7984696 - 财政年份:2010
- 资助金额:
$ 18.26万 - 项目类别:
Mechanisms and Implications of Nanoelectroporation in Living Cells
活细胞纳米电穿孔的机制和意义
- 批准号:
8500364 - 财政年份:2010
- 资助金额:
$ 18.26万 - 项目类别:
Mechanisms and Implications of Nanoelectroporation in Living Cells
活细胞纳米电穿孔的机制和意义
- 批准号:
8298579 - 财政年份:2010
- 资助金额:
$ 18.26万 - 项目类别:
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