Domestication and characterization of TM7-the most elusive oral phylum

TM7——最难以捉摸的口腔门的驯化和表征

• 批准号: 8999567
• 负责人: Xuesong He
• 金额: $ 3万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-04-01 至 2018-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8999567
• 关键词: Adherence; Area; Attention; Bacteria; Behavior; Biological; Biological Assay; Cell Death; Cell Separation; Cells; Coculture Techniques; Communities; Complex; Culture Media; Disease; Environment; Epithelial Cells; Equipment and supply inventories; Gene Cluster; Gene Expression; Genes; Genetic; Genome; Genomics; Gingiva; Goals; Growth; Health; Human; Human Microbiome; Human body; In Vitro; Inflammatory; Inflammatory Bowel Diseases; Laboratories; Life; Lymphocyte; Mass Spectrum Analysis; Metabolic; Metabolic Pathway; Microbiology; Mining; Modeling; Molecular; Names; Oral; Oral cavity; Pathogenesis; Pathway interactions; Periodontal Diseases; Periodontitis; Phenotype; Physiological; Production; Research; Research Personnel; Resources; Role; Site; Techniques; Testing; Transcript; Tube; Veillonella atypica; Virulence; Work; base; chemokine; functional genomics; improved; interest; member; microbial; microorganism; novel; oral bacteria; pathogen; research study; response; small molecule; tissue/cell culture; transcriptome sequencing

DESCRIPTION (provided by applicant): The human microbiome project has provided a comprehensive inventory of the microorganisms associated with the human body. The next challenge is to understand their role in human health and disease which requires cultivation of the species of interest. Relevant to this application, more than half of the oral microorganisms are categorized as uncultivated phylotypes including many associated with oral microbial pathogenesis. Among them, members of the candidate division TM7 phylum are the most enigmatic bacterial group in the oral cavity. Despite their ubiquitous occurrence in human body sites and other environments, no TM7 representative has been cultivated as pure culture. As a phylum containing suspected pathogens associated with inflammatory mucosal diseases, particularly periodontitis, cultivation of TM7 and the ability to investigate their pathogenic features has become like a "Holy Grail" in microbiology. Using a novel medium and enrichment techniques, we were able to reduce TM7-containing cultures from a complex community to dual-species co-cultures containing the disease associated TM7 phylotype (TM7x) together with Atopobium parvulum or Veillonella atypica as partner strain. We hypothesize that the growth of TM7x requires specific partner species and that understanding their relationship may be the key for isolating pure culture of TM7x. Furthermore, the present semi-pure cultures of TM7x and the possible pure TM7x cultures obtained through this study will enable our ability to study genomics, interactomics and the pathogenic potential of TM7x. This application aims to test our hypothesis and achieve these goals through detailed molecular analyses of the relationship between TM7x and its partner species, improving the medium for cultivating pure TM7x, as well as exploring its pathogenic potential. The immediate impact of the study is the discovery of cultivation conditions for TM7x and the associated biological/physiological functions. The long-term significance of the study is the creation of a new pathway for cultivation of many other yet-to-be cultured potentially pathogenic species.

描述（由申请人提供）：人体微生物组项目提供了与人体相关的微生物的全面清单。下一个挑战是了解它们在人类健康和疾病中的作用，这需要培养感兴趣的物种。与本申请相关，超过一半的口腔微生物被归类为未培养的微生物类型，包括许多与口腔微生物发病机制相关的微生物。其中，候选部门TM 7门的成员是口腔中最神秘的细菌群。尽管它们普遍存在于人体部位和其他环境中，但没有TM 7代表物被培养为纯培养物。作为含有与炎性粘膜疾病特别是牙周炎相关的可疑病原体的门，TM 7的培养和研究其致病特征的能力已经成为微生物学中的“圣杯”。使用一种新的培养基和富集技术，我们能够减少TM 7含有文化从一个复杂的社会双物种共培养物含有疾病相关的TM 7型（TM 7 x）与Atopobium parvulum或韦荣球菌作为合作伙伴菌株。我们推测TM 7 x的生长需要特定的伙伴物种，了解它们之间的关系可能是分离TM 7 x纯培养物的关键。此外，本研究获得的TM 7 x的半纯培养物和可能的纯TM 7 x培养物将使我们能够研究基因组学、相互作用和TM 7 x的致病潜力。本申请旨在验证我们的假设，并实现这些目标，通过详细的分子分析之间的关系TM 7 x和它的合作伙伴物种，改进培养基培养纯TM 7 x，以及探索其致病潜力。该研究的直接影响是发现了TM 7 x的培养条件和相关的生物/生理功能。这项研究的长期意义在于为培养许多其他尚未培养的潜在致病物种创造了一条新途径。