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Analytical Neurochemistry: Core D

分析神经化学：核心 D

基本信息

批准号：
9054633
负责人：
Michael Byrne Robinson
金额：
$ 19.12万
依托单位：
CHILDREN'S HOSP OF PHILADELPHIA
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-11-01 至 2020-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9054633
关键词：
Affect Amino Acids Animal Model Behavior Behavioral Biochemistry Bioenergetics Biogenic Amines Bioinformatics Biological Assay Biometry Blinded Brain Calcium Cataloging Catalogs Cells Cerebral Palsy Charge Chemicals Clinical Data Analyses Defect Developmental Disabilities Disease Electrophysiology (science)Eligibility Determination Epilepsy Experimental Designs Free Radicals Gene Mutation Genes Genetic Glutamates Goals High Pressure Liquid Chromatography Human Hypoxia Impaired cognition In Vitro Individual Inherited Intellectual functioning disability Investigation Kinetics Lead Mass Spectrum Analysis Measurement Measures Membrane Potentials Mental Retardation and Developmental Disabilities Research Centers Metabolic Metabolic Diseases Metabolic Pathway Mission Mitochondria Mitochondrial DNA Modeling Modification Motion Myelin Neurotransmitters Oxidative Phosphorylation Pathologic Pathology Patients Pattern Peptides Physiology Pre-Clinical Model Proteins Quality Control Reaction Regulation Reporting Reproducibility Research Residual state Sample Size Services System Technology Testing Therapeutic Intervention Tissues Tracer Variant biomarker development brain behavior disability glucose metabolism improved outcome in vivo leukodystrophy neurochemistry neuroimaging novel pre-clinical preference protein expression research study small molecule stable isotope therapy development urea cycle

项目摘要

(ANALYTICAL NEUROCHEMISTRY CORE (ANC): CORE D) PROJECT SUMMARY Description: The Analytical Neurochemistry Core (ANC) supports these services: (a) Quantitation of amino acids and biogenic amines; (b) Kinetic studies of flux through metabolic pathways using stable isotopes as tracers and mass spectrometry to measure isotopic enrichment; (c) Qualitative and quantitative mass spectroscopic analyses of proteins/peptides and their modifications; and (d) Bioenergetic assays of mitochondrial function. Analyses are performed: (a) In vitro; (b) In pre-clinical animal models of intellectual and developmental disabilities (IDD); and (c) In vivo, in human patients. Users have access to a broad repertoire of analytical services, as well as expert advice with regard to experimental design and data interpretation. The core emphasizes all aspects of quality control, including assurance that studies are adequately powered and properly controlled. When feasible, analyses are performed in a blinded fashion. Relevance to IDDRC Mission: The overall theme of our Center is – “Genes, Brain and Behavior”. The primary focus of the ANC is “Brain”, or the neurochemical changes which result from gene mutation and variation. These neurochemical aberrations may contribute to the behavioral anomalies that become clinical manifestations of IDD. The ANC enables users to identify and quantify changes in brain biochemistry. Such information deepens understanding of the IDD and affords development of biomarkers with which to gauge the efficacy of therapeutic interventions. Eligibility: These services are available both to approved users of the IDDRC at CHOP/UPenn and to users at other Centers in the Network.

（分析神经化学核心（anc）：核心d）