Analytical Neurochemistry: Core D

分析神经化学：核心 D

• 批准号: 9054633
• 负责人: Michael Byrne Robinson
• 金额: $ 19.12万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-11-01 至 2020-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9054633
• 关键词: Affect; Amino Acids; Animal Model; Behavior; Behavioral; Biochemistry; Bioenergetics; Biogenic Amines; Bioinformatics; Biological Assay; Biometry; Blinded; Brain; Calcium; Cataloging; Catalogs; Cells; Cerebral Palsy; Charge; Chemicals; Clinical; Data Analyses; Defect; Developmental Disabilities; Disease; Electrophysiology (science); Eligibility Determination; Epilepsy; Experimental Designs; Free Radicals; Gene Mutation; Genes; Genetic; Glutamates; Goals; High Pressure Liquid Chromatography; Human; Hypoxia; Impaired cognition; In Vitro; Individual; Inherited; Intellectual functioning disability; Investigation; Kinetics; Lead; Mass Spectrum Analysis; Measurement; Measures; Membrane Potentials; Mental Retardation and Developmental Disabilities Research Centers; Metabolic; Metabolic Diseases; Metabolic Pathway; Mission; Mitochondria; Mitochondrial DNA; Modeling; Modification; Motion; Myelin; Neurotransmitters; Oxidative Phosphorylation; Pathologic; Pathology; Patients; Pattern; Peptides; Physiology; Pre-Clinical Model; Proteins; Quality Control; Reaction; Regulation; Reporting; Reproducibility; Research; Residual state; Sample Size; Services; System; Technology; Testing; Therapeutic Intervention; Tissues; Tracer; Variant; biomarker development; brain behavior; disability; glucose metabolism; improved outcome; in vivo; leukodystrophy; neurochemistry; neuroimaging; novel; pre-clinical; preference; protein expression; research study; small molecule; stable isotope; therapy development; urea cycle

(ANALYTICAL NEUROCHEMISTRY CORE (ANC): CORE D)  PROJECT SUMMARY Description: The Analytical Neurochemistry Core (ANC) supports these services: (a) Quantitation of amino acids and biogenic amines; (b) Kinetic studies of flux through metabolic pathways using stable isotopes as tracers and mass spectrometry to measure isotopic enrichment; (c) Qualitative and quantitative mass spectroscopic analyses of proteins/peptides and their modifications; and (d) Bioenergetic assays of mitochondrial function. Analyses are performed: (a) In vitro; (b) In pre-clinical animal models of intellectual and developmental disabilities (IDD); and (c) In vivo, in human patients. Users have access to a broad repertoire of analytical services, as well as expert advice with regard to experimental design and data interpretation. The core emphasizes all aspects of quality control, including assurance that studies are adequately powered and properly controlled. When feasible, analyses are performed in a blinded fashion. Relevance to IDDRC Mission: The overall theme of our Center is – “Genes, Brain and Behavior”. The primary focus of the ANC is “Brain”, or the neurochemical changes which result from gene mutation and variation. These neurochemical aberrations may contribute to the behavioral anomalies that become clinical manifestations of IDD. The ANC enables users to identify and quantify changes in brain biochemistry. Such information deepens understanding of the IDD and affords development of biomarkers with which to gauge the efficacy of therapeutic interventions. Eligibility: These services are available both to approved users of the IDDRC at CHOP/UPenn and to users at other Centers in the Network.

（分析神经化学核心（ANC）：核心D） 项目摘要 描述：分析神经化学核心（ANC）支持这些服务：（a）氨基的定量 （B）使用稳定同位素作为放射性同位素对通过代谢途径的通量进行动力学研究， 示踪剂和质谱法，以测量同位素富集度;（c）定性和定量质量 蛋白质/肽及其修饰的光谱分析;以及（d） 线粒体功能进行以下分析：（a）体外;（B）在智力和智力发育的临床前动物模型中， 发育障碍（IDD）;和（c）在体内，在人类患者中。用户可以访问广泛的 分析服务，以及关于实验设计和数据解释的专家建议。的 核心强调质量控制的各个方面，包括保证研究具有足够的效力， 适当控制。可行时，以设盲方式进行分析。 与IDDRC使命的相关性：我们中心的总主题是-“基因，大脑和行为”。主 ANC的焦点是“脑”，即基因突变和变异导致的神经化学变化。 这些神经化学畸变可能导致临床上的行为异常 IDD的表现。ANC使用户能够识别和量化大脑生物化学的变化。等 信息加深了对IDD的理解，并提供了生物标志物的发展， 治疗干预的有效性。 资格：这些服务可供CHOP/UPenn的IDDRC批准用户和 网络中的其他中心。