Core B: Proteomics and Lipidomics Core

核心 B：蛋白质组学和脂质组学核心

• 批准号: 9210670
• 负责人: Calvin Pardee Hull Vary
• 金额: $ 24.26万
• 依托单位: MAINEHEALTH
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9210670
• 关键词: Address; Adipose tissue; Antipsychotic Agents; Applications Grants; Biochemical Pathway; Biological; Biology; Blood Vessels; Brown Fat; Cells; Centers of Research Excellence; Client; Clinical Trials; Communities; Complex; Computer software; Core Facility; Data; Data Analyses; Data Set; Development; Disease; Equipment; Evolution; Future; Gene Expression; Goals; Hybrids; Individual; Informatics; Institutes; Institution; Investments; Ions; Isotope Labeling; Isotopes; Isotopically-Coded Affinity Tagging; Link; Lipids; Liquid substance; Maine; Marrow; Mass Spectrum Analysis; Medical center; Mesenchymal; Metabolic; Methodology; Methods; Molecular; Multivariate Analysis; Obesity; Osteocytes; Osteoporosis; Outcome; Peptides; Positioning Attribute; Preparation; Protein Analysis; Proteins; Proteomics; Research; Research Infrastructure; Research Institute; Research Personnel; Resources; Sampling; Scanning; Scientific Inquiry; Serum; Services; Signal Transduction; Standardization; Statistical Methods; Techniques; Technology; Testing; Tissue Sample; Tissues; United States National Institutes of Health; Work; analytical method; atypical antipsychotic; base; biological systems; bone; bone loss; computing resources; data integration; improved; in vivo; innovation; insight; instrument; mass spectrometer; multiple reaction monitoring; neuroregulation; novel; novel strategies; programs; protein expression; protein phosphatase inhibitor-2; relating to nervous system; response; success; translational study

The long-term goals of the Proteomics and Lipidomics Core are to: (1) provide new approaches that expand previous protein identification capabilities, (2) provide isotope-free quantitative protein analysis, (3) enable broad unbiased lipid profiling of cells and tissues, (4) enable targeted lipid quantitation, and (5) improve statistical methods for data interpretation. This core facility was developed based on strategic focusing of targeted investments made in the past decade. The mass spectrometry resource was originally supported by our COBRE in Vascular Biology, and has steadily grown and been supported by new equipment and software investments by our institution. Recently, there has been a significant evolution of protein and lipid identification and quantification technologies, with corresponding maturation of data analysis methodology. This evolution has led to a major advance in the fields of proteomics and lipidomics. In the last year we have implemented these technologies based on the premise that quantitative protein and lipid profiling is critical for the success of the projects proposed in this COBRE and within our greater research community. We are now uniquely positioned as the only regional facility of its kind within the state of Maine providing these services for academic, not-for-profit, and for-profit scientific inquiry. Understanding the interconnection of bone and adipose gene expression and lipid metabolic changes in mammalian tissues and cells is fundamental to understanding disease. Thus, this resource will provide protein expression and lipid metabolic information on biological systems, and the intellectual and computational resources needed to guide COBRE studies. Two specific aims are proposed. Specific Aim 1. Provide state-of-the-art qualitative protein identification and quantitative protein expression analysis. We will offer standard and new mass spectrometry-based services for investigators for unbiased protein identification and quantitation of complex cell and tissue samples to support the projects in this COBRE. Specific Aim 2. Provide new capabilities for unbiased broad-spectrum lipid profiling and targeted characterization of cell and tissue lipids. We will provide investigators with state-of-the-art unbiased lipid profiling of diverse sample types, including cells, serum, and adipose tissue using ion scanning and fragmentation mass spectrometry of all precursor species. Successful implementation of Core services and integration with the projects in this COBRE will benefit investigators by providing high quality protein expression and lipid profiling data sets for their proposed projects and future grant applications. Insights from protein expression and lipid metabolic data will inform translational studies that could ultimately see application in clinical trials of individuals with osteoporosis and obesity.!

蛋白质组学和脂质组学核心的长期目标是：（1）提供新的方法， 以前的蛋白质鉴定能力，（2）提供无同位素定量蛋白质分析，（3）使 细胞和组织的广泛无偏脂质谱分析，（4）能够进行靶向脂质定量，和（5）改善 数据解释的统计方法。这一核心设施是根据以下战略重点开发的： 在过去十年中进行的投资。质谱分析资源最初是由 通过我们的COBRE在血管生物学，并已稳步增长，并得到新设备的支持， 我们的软件投资。近年来，蛋白质和脂质的结构发生了重大变化， 识别和量化技术，以及相应的数据分析方法的成熟。 这种演变导致了蛋白质组学和脂质组学领域的重大进展。去年，我们 已经基于定量蛋白质和脂质谱分析是 这对于COBRE和我们更大的研究社区中提出的项目的成功至关重要。 我们现在处于独特的地位，是缅因州唯一的区域设施， 这些服务的学术，不以营利为目的，以营利为目的的科学调查。理解互联 哺乳动物组织和细胞中骨和脂肪基因表达和脂质代谢变化的研究 是理解疾病的基础因此，该资源将提供蛋白质表达和脂质 生物系统的代谢信息，以及所需的智力和计算资源， 指导COBRE研究。提出了两个具体目标。 具体目标1.提供最先进的蛋白质定性鉴定和蛋白质定量 表情分析我们将为研究人员提供标准和新的基于质谱的服务， 对复杂细胞和组织样本进行无偏蛋白质鉴定和定量，以支持 这个眼镜蛇。 具体目标2。为无偏倚的广谱脂质分析和靶向 细胞和组织脂质的表征。我们将为研究者提供最先进的无偏脂质 使用离子扫描分析不同样品类型，包括细胞、血清和脂肪组织， 所有前体物质的裂解质谱。 成功实施核心服务并与此COBRE中的项目集成将使 研究人员通过提供高质量的蛋白质表达和脂质谱数据集， 项目和未来的赠款申请。从蛋白质表达和脂质代谢数据的见解将告知 转化研究，最终可以看到应用于骨质疏松症患者的临床试验， 肥胖！