Core B: Proteomics and Lipidomics Core

核心 B：蛋白质组学和脂质组学核心

• 批准号: 10246816
• 负责人: Calvin Pardee Hull Vary
• 金额: $ 19.82万
• 依托单位: MAINEHEALTH
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10246816
• 关键词: Address; Adipose tissue; Antipsychotic Agents; Applications Grants; Biochemical Pathway; Biological; Biology; Blood Vessels; Brown Fat; Cells; Centers of Research Excellence; Client; Clinical Trials; Communities; Complex; Computer software; Core Facility; Data; Data Analyses; Data Set; Development; Disease; Equipment; Evolution; Future; Gene Expression; Goals; Hybrids; Individual; Informatics; Infrastructure; Institutes; Institution; Investments; Ions; Isotope Labeling; Isotopes; Isotopically-Coded Affinity Tagging; Letters; Link; Lipids; Liquid substance; Maine; Marrow; Mass Spectrum Analysis; Medical center; Mesenchymal; Metabolic; Methodology; Methods; Molecular; Multivariate Analysis; Obesity; Osteocytes; Osteoporosis; Outcome; Peptides; Positioning Attribute; Preparation; Protein Analysis; Proteins; Proteomics; Research; Research Institute; Research Personnel; Resources; Sampling; Scanning; Scientific Inquiry; Serum; Services; Signal Transduction; Standardization; Statistical Methods; Techniques; Technology; Testing; Tissue Sample; Tissues; United States National Institutes of Health; Work; analytical method; atypical antipsychotic; base; biological systems; bone; bone loss; computing resources; data integration; improved; in vivo; innovation; insight; instrument; lipidomics; mass spectrometer; multiple reaction monitoring; neuroregulation; novel; novel strategies; programs; protein expression; protein phosphatase inhibitor-2; relating to nervous system; response; success; translational study

The long-term goals of the Proteomics and Lipidomics Core are to: (1) provide new approaches that expand previous protein identification capabilities, (2) provide isotope-free quantitative protein analysis, (3) enable broad unbiased lipid profiling of cells and tissues, (4) enable targeted lipid quantitation, and (5) improve statistical methods for data interpretation. This core facility was developed based on strategic focusing of targeted investments made in the past decade. The mass spectrometry resource was originally supported by our COBRE in Vascular Biology, and has steadily grown and been supported by new equipment and software investments by our institution. Recently, there has been a significant evolution of protein and lipid identification and quantification technologies, with corresponding maturation of data analysis methodology. This evolution has led to a major advance in the fields of proteomics and lipidomics. In the last year we have implemented these technologies based on the premise that quantitative protein and lipid profiling is critical for the success of the projects proposed in this COBRE and within our greater research community. We are now uniquely positioned as the only regional facility of its kind within the state of Maine providing these services for academic, not-for-profit, and for-profit scientific inquiry. Understanding the interconnection of bone and adipose gene expression and lipid metabolic changes in mammalian tissues and cells is fundamental to understanding disease. Thus, this resource will provide protein expression and lipid metabolic information on biological systems, and the intellectual and computational resources needed to guide COBRE studies. Two specific aims are proposed. Specific Aim 1. Provide state-of-the-art qualitative protein identification and quantitative protein expression analysis. We will offer standard and new mass spectrometry-based services for investigators for unbiased protein identification and quantitation of complex cell and tissue samples to support the projects in this COBRE. Specific Aim 2. Provide new capabilities for unbiased broad-spectrum lipid profiling and targeted characterization of cell and tissue lipids. We will provide investigators with state-of-the-art unbiased lipid profiling of diverse sample types, including cells, serum, and adipose tissue using ion scanning and fragmentation mass spectrometry of all precursor species. Successful implementation of Core services and integration with the projects in this COBRE will benefit investigators by providing high quality protein expression and lipid profiling data sets for their proposed projects and future grant applications. Insights from protein expression and lipid metabolic data will inform translational studies that could ultimately see application in clinical trials of individuals with osteoporosis and obesity.!

蛋白质组学和脂质组学核心的长期目标是：(1)提供可扩展的新方法 以前的蛋白质识别能力，(2)提供无同位素的定量蛋白质分析，(3)使 广泛的无偏见的细胞和组织的脂类分布，(4)实现有针对性的脂类定量，以及(5)改善 数据解释的统计方法。该核心设施是基于以下战略重点开发的 过去十年中进行的有针对性的投资。最初支持的是质谱仪资源 由我们的血管生物学科布雷公司开发，并稳步增长，并得到了新设备和 我们机构的软件投资。最近，蛋白质和脂肪有了显著的进化。 识别和量化技术，相应成熟的数据分析方法。 这一进化导致了蛋白质组学和脂类组学领域的重大进展。在过去的一年里我们 我实施这些技术的前提是定量的蛋白质和脂肪图谱 对于在科布雷和我们更大的研究社区内提出的项目的成功至关重要。 我们现在的独特定位是缅因州内唯一的区域设施，提供 这些服务是为学术、非营利性和营利性科学研究提供的。了解互联互通 哺乳动物组织和细胞中骨和脂肪基因表达和脂代谢变化的研究 是了解疾病的基础。因此，这种资源将提供蛋白质表达和脂肪 关于生物系统的代谢信息，以及所需的智力和计算资源 指导科布雷的研究。提出了两个具体目标。 具体目标1.提供最先进的定性蛋白质鉴定和定量蛋白质 表情分析。我们将为调查人员提供标准和新的基于质谱学的服务 对复杂的细胞和组织样本进行无偏见的蛋白质鉴定和定量，以支持 这只科布雷。 具体目标2.为无偏见的广谱脂类分析和靶向提供新的能力 细胞和组织脂质的表征。我们将为调查人员提供最先进的无偏见的脂质 使用离子扫描和分析不同类型的样本，包括细胞、血清和脂肪组织 所有前体物种的裂解质谱图。 成功实施核心服务并与此Cobre中的项目集成将受益 研究人员通过为他们的建议提供高质量的蛋白质表达和脂谱数据集 项目和未来的赠款申请。对蛋白质表达和脂肪代谢数据的洞察将为 翻译研究最终可能在骨质疏松症患者的临床试验中得到应用 肥胖症！