Proteomics and Lipidomics Core
Proteomics and Lipidomics Core
批准号:
10711696
负责人:
Calvin Pardee Hull Vary
金额:
$30.92万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2017
资助国家:
美国
项目状态:
未结题
起止时间:
2017-09-01 至 2028-08-31
关键词:
AddressAdipocytesApplications GrantsAwardBasic ScienceBiochemical PathwayBiologicalBiomedical EngineeringBone remodelingCardiometabolic DiseaseCardiovascular systemCellsCenters of Research ExcellenceClientClinical ResearchComplexCore FacilityDataData AnalysesData CollectionDevelopmentDoctor of PhilosophyEnsureEquipmentExperimental DesignsFundingFutureGoalsGrantHumanInformaticsInstitutionInvestmentsIonsIsotopesLipidsLiquid ChromatographyLiquid substanceMass ChromatographyMass Spectrum AnalysisMesenchymalMetabolicMethodologyMolecularMonitorMusNational Institute of General Medical SciencesOutcomePeptidesPhasePhysiologicalPilot ProjectsPositioning AttributePostdoctoral FellowPreparationPropertyProtein AnalysisProteinsProteomeProteomicsProtocols documentationPublishingReactionResearchResearch DesignResearch InstituteResearch PersonnelResearch Project GrantsResolutionResourcesRisk FactorsRoleSamplingScanningScientific InquirySerumServicesSignal TransductionSpecimenStatistical Data InterpretationStatistical MethodsStudentsTechnologyTissue EngineeringTissue SampleTissuesTrainingTranslational ResearchUnited States National Institutes of HealthWorkbariatric surgerybone lossdata acquisitiondata integrationdata repositoryenergy balanceequipment acquisitionexperiencefacility renovationflexibilityhuman tissueimprovedinnovationinsightinstrumentinstrumentationlarge datasetslipidomelipidomicsliquid chromatography mass spectrometrymass spectrometermodifiable riskmultiple reaction monitoringneuroregulationnovelnovel strategiesprogramsprotein expressionrecruitresponsesuccess
中文摘要
蛋白质组学和脂质组学核心(C. Vary,核心主任)
英文摘要
Proteomics and Lipidomics Core (C. Vary, Core Director)
The Proteomics and Lipidomics Core is led by Calvin Vary PhD and provides high quality research design
support, sample preparation, mass spectrometry analysis, data collection, and data analysis for a variety of
tissues and cells. As a component of the COBRE in Mesenchymal and Neural Regulation of Metabolic
Networks since 2017, our services have focused on protein and lipid profiling in mouse and human tissues.
Our goals remain to: 1) provide new approaches that expand previous protein identification capabilities, 2)
provide unbiased analytic workflows for proteomics and lipidomics studies that include 3) further developing
targeted protein (e.g., MRMHR, SRM) and lipid quantitation (MSMSALL, MPIS) workflows, and 4) provide
improved statistical and informatics approaches for data interpretation and identification of mechanisms.
In Phase I of this COBRE, the Proteomics and Lipidomics Core consistently supported basic, translational, and
clinical research projects and grant applications. These have included young investigator-initiated COBRE
projects, COBRE pilot projects, and other NIH research applications, both within and outside of MaineHealth
Institute for Research. A significant Phase I accomplishment was the award of an NIH S10 shared instrument
grant in support of a new high resolution TripleToF mass spectrometer, liquid chromatograph, and other
support equipment. MaineHealth provided institutional funds to renovate the facility, which consolidated the
current and new equipment in a more efficient footprint. This support has increased our capacity, efficiency,
and flexibility by allowing expanded proteomics and lipidomics workflows and increased user access, while
providing new opportunities to support ongoing research and training for internal and external users. In phase
I, we recruited an outstanding mass spectrometry technology manager, Carlos Gartner PhD, who brought
expertise that includes mass spectrometry instrumentation and advanced liquid chromatography. We also
recruited Yulica Santos-Ortega, PhD as a research associate with mass spectrometry-based cardiovascular
proteomics experience. New Phase II opportunities include exploitation of human proteome-wide selected
reaction monitoring protocols using on-line data repositories that will be implemented on new instrumentation
(Sciex QTRAP 6500+). We continue to be uniquely positioned as the only regional facility of its kind providing
these services for scientific inquiry and in support of student, investigator, and staff training. Within this
COBRE, these resources will be critical for promoting junior investigator advancement. This core will advance
our understanding of the properties of thermogenic adipocyte lineages and roles in energy balance (Project 1,
M. Lynes), provide insight into the role of the X/A cell secretome on bariatric surgery-related bone loss (Project
2, Z. Li). This core will also be well positioned to support PI progress and future exploratory directions for
identification of modifiable risk factors in cardiometabolic disease (Project 3, I. Agarwal).
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
Core B: Proteomics and Lipidomics Core
-
批准号:9210670
-
项目类别:
-
资助金额:$24.26万
-
财政年份:2017
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
Core B: Proteomics and Lipidomics Core
-
批准号:10246816
-
项目类别:
-
资助金额:$19.82万
-
财政年份:2017
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
CORE B PROTEIN, NUCLEIC ACID ANALYSIS AND CELL IMAGING
-
批准号:7959653
-
项目类别:
-
资助金额:$24.07万
-
财政年份:2009
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
Regulation of TGF-beta Receptor-dependent Vascular Disease
-
批准号:7838891
-
项目类别:
-
资助金额:$26.14万
-
财政年份:2009
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
CORE B PROTEIN, NUCLEIC ACID ANALYSIS AND CELL IMAGING
-
批准号:7720093
-
项目类别:
-
资助金额:$23.42万
-
财政年份:2008
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
CORE B PROTEIN, NUCLEIC ACID ANALYSIS AND CELL IMAGING
-
批准号:7609687
-
项目类别:
-
资助金额:$27.17万
-
财政年份:2007
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
Regulation of TGF-beta Receptor-dependent Vascular Disease
-
批准号:7586701
-
项目类别:
-
资助金额:$34.52万
-
财政年份:2007
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
Regulation of TGF-beta Receptor-dependent Vascular Disease
-
批准号:7448001
-
项目类别:
-
资助金额:$2.69万
-
财政年份:2007
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
Regulation of TGF-beta Receptor-dependent Vascular Disease
-
批准号:7262739
-
项目类别:
-
资助金额:$34.52万
-
财政年份:2007
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
SMAD-INDEPENDENT TGF-BETA SIGNALING MECHANISMS IN ANGIOGENESIS
-
批准号:7609693
-
项目类别:
-
资助金额:$18.81万
-
财政年份:2007
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
Regulation of TGF-beta Receptor-dependent Vascular Disease
-
批准号:7797526
-
项目类别:
-
资助金额:$34.52万
-
财政年份:2007
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
Regulation of TGF-beta Receptor-dependent Vascular Disease
-
批准号:7393722
-
项目类别:
-
资助金额:$34.52万
-
财政年份:2007
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
SMAD-INDEPENDENT TGF-BETA SIGNALING MECHANISMS IN ANGIOGENESIS
-
批准号:7381070
-
项目类别:
-
资助金额:$19.21万
-
财政年份:2006
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
CORE B PROTEIN, NUCLEIC ACID ANALYSIS AND CELL IMAGING
-
批准号:7381064
-
项目类别:
-
资助金额:$30.74万
-
财政年份:2006
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
COBRE: MMC: STRUCTURAL BIOLOGY(CORE B):POSTTRANSLATIONAL
-
批准号:7170227
-
项目类别:
-
资助金额:$13.14万
-
财政年份:2005
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
COBRE: MMC: ENDOGLIN IN ANGIOGENESIS; PROTEIN-COLLAGEN
-
批准号:7170225
-
项目类别:
-
资助金额:$13.88万
-
财政年份:2005
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
COBRE: MMC: STRUCTURAL BIOLOGY (CORE B): POST TRANSLATIONAL MODIFICATION
-
批准号:7011645
-
项目类别:
-
资助金额:$26.46万
-
财政年份:2004
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
COBRE: MMC: ENDOGLIN IN ANGIOGENESIS; PROTEIN IN COLLAGEN GELS, ANGIOGENESIS
-
批准号:7011643
-
项目类别:
-
资助金额:$31.77万
-
财政年份:2004
-
负责人:Calvin Pardee Hull Vary
-
依托单位:
国内基金
海外基金
支链氨基酸代谢紊乱调控“Adipocytes - Macrophages Crosstalk”诱发2型糖尿病脂肪组织功能和结构障碍的作用及机制
-
批准号:81970721
-
项目类别:面上项目
-
资助金额:55.0万元
-
批准年份:2019
-
负责人:陶凌
-
依托单位: