Innovative Technology and Instrumentation (ITI) Core

项目摘要 NEI赞助的Stein Eye Institute(SEI)研究人员呼吁 发展和改进创新的光谱、电生理、光学和 机械系统。为了满足这一需求，一种最先进的计算机数字控制 (数控)制造和工程设施已在SEI建立。这一设施使 建造新的设备和仪器，引导SEI调查人员进入未知领域 和激动人心的研究领域。例如，哈贝尔博士需要设计和建造 电子顺磁共振(EPR)光谱仪在高静水压下的应用 揭示视觉信号转导中蛋白质功能动力学的压力。霍维茨博士的 研究需要定制用于远、近采样的圆二色光谱仪 高压下晶状体蛋白质的紫外光CD。SamPath博士需要定制的荧光材料 用来记录视网膜细胞的单光子反应的显微镜。格拉斯哥博士的研究 需要定制的荧光仪以及单分子共焦和超分辨率 用于测量泪液各层中蛋白质动力学寿命荧光的显微镜 电影。德默博士的研究需要定制的控制系统来跟踪眼睛 研究斜视患者的运动和定制的机械装置 测量眼外肌张力和弹性。对于这些示例中的每个， 仪器设备不是商业上可用的，而是重新设计和建造的，或者 对现有设备进行了广泛的改造。这一组成部分对PI的研究至关重要，因为 为接受过数控加工培训的工程人员提供支持。此外， 组件将提供支持普通生物化学和分子生物学的仪器 SEI实验室的需求，以及维护和维修现有的高技术仪器 核心实验室。

Project Summary The NEI sponsored research of investigators at the Stein Eye Institute (SEI) calls for the development and modification of innovative spectroscopic, electrophysiological, optical and mechanical systems. To support this need, a state-of-the-art computer numerical controlled (CNC) fabrication and engineering facility has been established at the SEI. This facility enables the construction of novel devices and instruments that lead SEI investigators into unexplored and exciting areas of research. For example, Dr. Hubbell requires the design and construction of Electron Paramagnetic Resonance (EPR) spectrometers for operation at high hydrostatic pressure to reveal functional dynamics of proteins in visual signal transduction. Dr. Horwitz's research demands circular dichroism spectrometers customized for sampling both far and near UV CD of lens proteins at high pressure. Dr. Sampath requires customized fluorescent microscopes built for recording single photon responses of retinal cells. Dr. Glasgow's research demands customized fluorometers as well as single molecule confocal and super-resolution microscopes for measuring lifetime fluorescence of protein dynamics in the layers of the tear film. Dr. Demer's research necessitates customized control systems for tracking eye movements in patients to study strabismus and well as customized mechanical devices for measuring extraocular muscle tension and elasticity. For each of these examples, instrumentation is either not available commercially and is designed and built de novo, or existing equipment is extensively modified. This component is essential to the PI's research by providing support for engineering personnel trained in CNC fabrication. In addition, the component will provide instrumentation to support common biochemistry and molecular biology needs of SEI laboratories and maintain and repair existing high technology instrumentation in the core laboratory.