TRD #1: New approaches to specimen preparation

2.TRD#1：标本制备的新方法 摘要 我们正在开发新的样品制备方法，这些方法比传统的方法有几个优点 方法：研究方法。这些优势包括消耗非常少量的样品，并提供更多的样品 对准备好的格栅的质量进行控制。该系统使用一种名为Spotiton的机器人设备来分配 将体积在纳升数量级的液滴滴到EM栅格上的精确位置。该设备的成功之处在于 再加上我们开发的一种新的“自吸墨”网格，它提供了一种将样本扩散到薄层的方法 无需使用外部涂抹的滤纸即可制成薄膜。在未来5年内，我们将设计、开发、测试和 验证能够完成多种任务的通用Spotiton系统，包括制作良好控制的玻璃化网格， 管理时间分辨和快速混合实验，并准备~100独立负染 单个栅格上的采样。我们预计这款设备的一个版本将由商业广告生产 公司，以使其成为研究社区普遍可用的。

2. TRD#1: New Approaches to Specimen Preparation Abstract We are developing novel methods for specimen preparation that provide several advantages over traditional methods. These advantages include consumption of very small amounts of sample, and providing for more control over the quality of the prepared grids. The system uses a robotic device, called Spotiton, to dispense drops with volumes on the order of nanoliters to precise locations on an EM grid. The success of the device is coupled to a new “self-blotting” grid that we have developed to provide a method for spreading sample to a thin film without the use of externally applied filter paper. Over the next 5 years we will design, develop, test and validate a generalized Spotiton system capable of multiple tasks including making well controlled vitrified grids, managing time resolved and fast mixing experiments, and preparing ~100 independent negatively stained samples on a single grid. We anticipate that a version of this device will be manufactured by a commercial company so that it becomes generally available to the research community.