Center for Photogenomics

光基因组学中心

• 批准号: 9338283
• 负责人: JOHN A STAMATOYANNOPOULOS
• 金额: $ 200万
• 依托单位: ALTIUS INSTITUTE FOR BIOMEDICAL SCIENCES
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-14 至 2020-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9338283
• 关键词: Alpha Cell; Back; Biochemical; Biological; Cell Nucleus; Cells; Chemicals; Chemistry; Chromatin; Chromatin Structure Alteration; Clinical; DNA; DNA-Binding Proteins; Detection; Development; Elements; Engineering; Enhancers; Ensure; Environment; Foundations; Generations; Genetic Fingerprintings; Genome; Genomics; Glucocorticoids; Human; Human Genome; Hypersensitivity; Image; Image Analysis; Imagery; Imaging Device; Imaging Techniques; In Situ; Individual; Knowledge; Laboratories; Lateral; Marrow; Methods; Microscope; Microscopy; Mission; Modeling; Modernization; Modification; Nuclear; Nucleic Acid Regulatory Sequences; Oligonucleotides; Optics; Pattern; Postdoctoral Fellow; Property; Quantum Dots; RNA; Regulatory Element; Reporting; Reproducibility; Research; Research Personnel; Resolution; Signal Transduction; Site; Solid; Specimen; Speed; Structure; Students; Techniques; Technology; Tissues; Training Programs; Translations; base; cell type; clinical diagnostics; clinically relevant; fluorophore; in vivo; innovation; instrument; instrumentation; multidisciplinary; nanocrystal; new technology; non-genomic; novel; outreach program; peripheral blood; pre-doctoral; programs; public health relevance; tissue preparation; transcription factor

 DESCRIPTION (provided by applicant): The mission of the Center for Photogenomics is to develop revolutionary technologies that enable the direct visualization and functional profiling of human regulatory regions at the resolution of individual chromatin templates in intact cells, and to leverage the extraordinary cell selectivity and information content of regulatory DNA to pioneer novel biological and translational applications. The Center will specifically (i) develop technology to simultaneously visualize and localize in highly multiplexed fashion, regulatory DNA regions on individual chromatin templates within the intact cell nucleus; (ii) develop technology for activity-based profiling of regulatory regions; (iii) pioneer structural, functional and integrative applications of photogenomics using super-resolution "nanoscopic" techniques; (iv) enable photogenomics through development of revolutionary instrumentation for high-throughput, high-speed super-resolution microscopy; and (v) lay the foundation for translation of photogenomic techniques to solving common problems in the modern clinical diagnostic laboratory by integrating with existing clinical workflows. This will ensure that photogenomics wil bring the power of genomic analysis to the understanding of cells within the context of their tissue environment. In keeping with the mission of the CEGS program of training and outreach, this Center proposal outlines a strong multi-disciplinary post- doctoral program that will create a new breed of genomics researcher with expertise in genomics as well as advanced imaging techniques and analysis. In addition, through innovative and interactive programs, the Center will expose non-genomics researchers and pre-doctoral students to this new field.

 描述(申请人提供)：摄影基因组学中心的使命是开发革命性的技术，使直接可视化和功能剖析成为可能 该技术能够在完整细胞中分辨出单个染色质模板，并利用调控DNA非凡的细胞选择性和信息含量，开创新颖的生物和翻译应用领域。该中心将具体(I)开发以高度多样化的方式同时显示和定位调控DNA区域的技术；(Ii)开发基于活动的调控区域图谱的技术；(Iii)利用超分辨率“纳米”技术开创光基因组学的结构、功能和集成应用；(Iv)通过开发用于高通量、高速超分辨率显微镜的革命性仪器，使光基因组学得以实现；以及(V)通过与现有临床工作流程相结合，为光基因组技术的转化奠定基础，以解决现代临床诊断实验室中的常见问题。这将确保光基因组学将带来基因组分析的力量，以了解细胞在其组织环境的背景下。为了与CEGS培训和推广计划的使命保持一致，该中心提案概述了一个强大的多学科博士后计划，将创造一个 拥有基因组学专业知识以及先进成像技术和分析的新一代基因组学研究人员。此外，通过创新和互动项目，该中心将让非基因组学研究人员和博士前学生接触到这一新领域。