Center for Photogenomics

光基因组学中心

• 批准号: 8883312
• 负责人: JOHN A STAMATOYANNOPOULOS
• 金额: $ 200万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-14 至 2016-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8883312
• 关键词: Back; Biochemical; Biological; Breeding; Cell Nucleus; Cells; Chemicals; Chemistry; Chromatin; Chromatin Structure Alteration; Clinical; DNA; DNA-Binding Proteins; Detection; Development; Diagnostic; Education and Outreach; Engineering; Enhancers; Ensure; Environment; Foundations; Generations; Genetic Fingerprintings; Genome; Genomics; Glucocorticoids; Human; Human Genome; Image; Image Analysis; Imagery; Imaging Device; Imaging Techniques; In Situ; Indium; Individual; Knowledge; Laboratories; Lateral; Marrow; Methods; Microscope; Microscopy; Mission; Modeling; Modification; Nuclear; Nuclear Localization Signal; Nucleic Acid Regulatory Sequences; Oligonucleotides; Optics; Pattern; Postdoctoral Fellow; Property; Quantum Dots; RNA; Regulatory Element; Reporting; Reproducibility; Research; Research Personnel; Resolution; Site; Solid; Specimen; Speed; Structure; Techniques; Technology; Tissues; Translations; base; cell type; clinically relevant; doctoral student; fluorophore; in vivo; innovation; instrument; instrumentation; nanocrystal; new technology; non-genomic; novel; peripheral blood; pre-doctoral; programs; public health relevance; tissue/cell preparation; transcription factor

 DESCRIPTION (provided by applicant): The mission of the Center for Photogenomics is to develop revolutionary technologies that enable the direct visualization and functional profiling of human regulatory regions at the resolution of individual chromatin templates in intact cells, and to leverage the extraordinary cell selectivity and information content of regulatory DNA to pioneer novel biological and translational applications. The Center will specifically (i) develop technology to simultaneously visualize and localize in highly multiplexed fashion, regulatory DNA regions on individual chromatin templates within the intact cell nucleus; (ii) develop technology for activity-based profiling of regulatory regions; (iii) pioneer structural, functional and integrative applications of photogenomics using super-resolution "nanoscopic" techniques; (iv) enable photogenomics through development of revolutionary instrumentation for high-throughput, high-speed super-resolution microscopy; and (v) lay the foundation for translation of photogenomic techniques to solving common problems in the modern clinical diagnostic laboratory by integrating with existing clinical workflows. This will ensure that photogenomics wil bring the power of genomic analysis to the understanding of cells within the context of their tissue environment. In keeping with the mission of the CEGS program of training and outreach, this Center proposal outlines a strong multi-disciplinary post- doctoral program that will create a new breed of genomics researcher with expertise in genomics as well as advanced imaging techniques and analysis. In addition, through innovative and interactive programs, the Center will expose non-genomics researchers and pre-doctoral students to this new field.

 描述（由申请人提供）：光基因组学中心的使命是开发革命性的技术，使直接可视化和功能分析， 人类调控区在完整细胞中的单个染色质模板的分辨率，并利用非凡的细胞选择性和调控DNA的信息内容，开拓新的生物和翻译应用。该中心将具体（i）开发技术，以高度多路复用的方式同时可视化和定位完整细胞核内单个染色质模板上的调控DNA区域;（ii）开发基于活性的调控区域分析技术;（iii）使用超分辨率“纳米”技术开拓光基因组学的结构、功能和综合应用;（iv）开发基于活性的调控区域分析技术。（iv）通过开发高通量、高速超分辨率显微镜的革命性仪器，实现光基因组学;以及（v）通过与现有临床工作流程相结合，为将光基因组学技术转化为解决现代临床诊断实验室中的常见问题奠定基础。这将确保光基因组学将把基因组分析的力量带到细胞组织环境背景下的细胞理解中。为了与CEGS培训和推广计划的使命保持一致，该中心的建议概述了一个强大的多学科博士后计划，将创建一个 新一代的基因组学研究人员，具有基因组学方面的专业知识以及先进的成像技术和分析。此外，通过创新和互动计划，该中心将使非基因组学研究人员和博士生接触这一新领域。