Illumina HiSeq 2500 Sequencer

Illumina HiSeq 2500 测序仪

• 批准号: 8639672
• 负责人: JOHN A STAMATOYANNOPOULOS
• 金额: $ 60万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2015-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8639672
• 关键词: Bioinformatics; Cells; Chemistry; Chromatin; Chromatin Structure; Communities; Core Facility; Coupled; DNA Methylation; Data; Funding; Genetic Transcription; Genome; Genomics; Growth; Massive Parallel Sequencing; Modification; Outcome; Output; Positioning Attribute; Price; Reading; Request for Proposals; Research; Research Personnel; Retroviral Vector; Running; Services; Speed; Technology; Time; Translating; Variant; base; cost; density; epigenomics; functional genomics; histone modification; instrument; meetings; next generation; next generation sequencing

DESCRIPTION (provided by applicant): The current proposal requests funds for the purchase of Illumina HiSeq 2500 sequencer, a next- generation, massively parallel sequencing platform. The ultra-high throughput of Illumina instruments, coupled with substantially reduced sequencing costs have made this a platform of choice for interrogating regulatory genomic sequences and function. Massively parallel sequencing is particularly well-suited for epigenomic and functional genomic studies, including chromatin structure, Chromatin accessibility, histone modifications and variants, regulatory factor localization; DNA methylation; transcription; and quantification of genome modifications such as localization of retroviral vector integrations. HiSeq2500 sequencer has dual flow cells with high density clusters generated in each channel that produces high quality passing filter data of 600 GB per 2X100 bp run. The two flow cells can be operated independently using different chemistries. The sequencer has the capability to operate in dual mode; in rapid run mode the clusters are generated on the sequencer, allows producing up to single or paired end 150 base long reads, and run times are several fold shorter , while in high output mode the sequence yields are up to 600 Gb per run. This allows tailoring the sequence run to the desired outcome in terms of speed vs. significantly large data output. The advances in Illumina sequencing technologies and chemistries, the price per Mb sequence output has made HiSeq a platform of choice for functional genomics research, These advances have led to explosive growth in demand for massively parallel sequencing, as more investigators realize the potential of the technology to impact and accelerate their research. The new instrument will be deployed in the context of a well-established, self-supporting core facility that already provides substantial epigenomics-focused next-generation sequencing services and associated bioinformatics support, and is therefore ideally positioned to rapidly translate the HiSeq 2500 potential to meet the needs of specific investigator projects as well as those of the general research community.

描述（由申请人提供）：目前的提案要求资金用于购买Illumina HiSeq 2500测序仪，这是一种下一代大规模并行测序平台。Illumina仪器的超高通量，加上大大降低的测序成本，使其成为查询调控基因组序列和功能的首选平台。大规模平行测序特别适用于表观基因组和功能基因组研究，包括染色质结构、染色质可及性、组蛋白修饰和变体、调节因子定位; DNA甲基化;转录;以及基因组修饰的定量，例如逆转录病毒载体整合的定位。HiSeq 2500测序仪具有双流动池，每个通道中产生高密度簇，每2X 100 bp运行产生600 GB的高质量通过过滤器数据。两个流动池可以使用不同的化学物质独立操作。测序仪具有在双模式下操作的能力;在快速运行模式下，在测序仪上生成簇，允许产生高达单端或双端150个碱基长的读取，并且运行时间缩短数倍，而在高输出模式下，每次运行的序列产量高达600 Gb。这允许在速度与显著大的数据输出方面将序列运行定制为期望的结果。Illumina测序技术和化学的进步，每Mb序列输出的价格使HiSeq成为功能基因组学研究的首选平台，这些进步导致了大规模并行测序需求的爆炸性增长，因为越来越多的研究人员意识到该技术的潜力，以影响和加速他们的研究。新工具将在一个完善的、自我维持的核心基金范围内部署 该公司已经提供了大量以表观基因组学为重点的下一代测序服务和相关的生物信息学支持，因此非常适合快速翻译 HiSeq 2500有潜力满足特定研究项目以及一般研究社区的需求。