Core 4: Laboratory p354-364

项目负责人/主要研究者（最后，第一，中间）：Khshnan-Sarin，Suchltra/O 'Malley，Stephanie S. 具体目标：本实验室核心的目的是支持在以下机构的主持下开展的研究： 建议的中心通过提供接触到选定的烟草相关的客观和定量的估计 药物/化合物，如其在生物液体中的浓度所反映的。符合优先事项之一 在FOA中，建议的项目涉及通过更好地了解如何减少成瘾， 烟草制品中某些普遍的成分变化可能会影响尼古丁成瘾的风险。 研究策略包括通过各种途径单独施用尼古丁，以及与其他药物联合施用。 选择的添加剂，包括薄荷醇，可能会影响对尼古丁的反应。研究的最佳解释 结果要求个体和实验性尼古丁和/或薄荷醇暴露的变化 武器可以考虑。分析物靶标的初始列表包括尼古丁、可替宁、反式3-OH可替宁/可替宁 (OHC/C）比率，薄荷醇葡糖苷酸和未结合的薄荷醇（用于体外溶液）。但 实验室核心将有能力根据中心不断变化的需求建立其他测定......

Program Director/Principal Investigator (Last, First, Middle): Khshnan-Sarin, Suchltra/O'Malley, Stephanie S. SPECIFIC AIMS: The purpose of this Laboratory Core is to support research carried out under the auspices of the proposed Center by providing objective and quantitative estimates of exposure to selected tobacco related drugs /compounds, as reflected by their concentrations in biological fluids. Consistent with one of the priorities in the FOA, the proposed projects are concerned with reducing addiction through better understanding of how , some prevalent modifications in the content of tobacco products may influence risk of addiction to nicotine. Research strategies include administration, by various routes, of nicotine alone, and in conjunction with other selected additives, including menthol, that may influence responses to nicotine. Optimal interpretation of study outcomes requires that variations in nicotine and/or menthol exposure across individuals and experimental arms be considered. The initial list of analyte targets includes nicotine, cotinine, trans 3-OH cotinine/cotinine (OHC/C) ratios, menthol glucuronide and unconjugated menthol (for in-vitro solutions). However, the Laboratory Core will have the ability to set up other assays as mandated by the evolving needs of the center...