Development of an spf Macaca nemestrina breeding colony

spf 猕猴线虫繁殖群的开发

• 批准号: 9348723
• 负责人: ROBERT J. ADAMS
• 金额: $ 97.15万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-21 至 2021-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9348723
• 关键词: AIDS/HIV problem; Animals; Breeding; Cercopithecine Herpesvirus 1; Data; Development; Disease Management; Family suidae; Female; Funding; Genetic; Genetic Markers; Genome; Genomic approach; Genotype; Goals; Grant; Hereditary Disease; Histocompatibility; Immunogenetics; Institution; Macaca; Macaca nemestrina; Maintenance; Research; Research Personnel; Resources; SIV; Sales; Serological; Simian Retroviruses; Single Nucleotide Polymorphism; Tail; Technology; Testing; United States; United States National Institutes of Health; Universities; Viral; Virus; animal resource; functional genomics; genetic pedigree; germ free condition; health data; human disease; male; member; offspring; pig genome; research study; whole genome

This renewal of our animal resource grant (P40 OD013117) requests five years of funding to continue support for a Specific Pathogen Free (SPF) colony of pig-tailed macaques (Macaca nemestrina) at the Johns Hopkins University that was first funded in 2006 and renewed in 2011. It has the following resource component and research component aims: A. Resource Component Specific Aims: Resource Aim 1: To expand the size of the current SPF breeding colony to increase the numbers of females for breeding and males available for sale to NIH funded investigators both within Johns Hopkins University and at other research institutions. Resource Aim 2: To maintain the SPF status of the colony by regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV). At present all of the current members of the colony have repeatedly tested negative for these agents. Resource Aim 3: To maintain pedigree and health data on all animals. This data is available on all of the animals in the colony and will be expanded as the colony grows. It is provided to purchasers of our animals. B. Research Component Specific Aims: To expand the genetic data available for pigtailed macaques to a) increase the research utility of pigtailed macaques and b) assist in genetic and disease management of the colony. This will be done through three research aims. Research Aim 1: To characterize pig-tailed macaque immunogenetics in detail via MHC class I and MHC class II genotyping. Research Aim 2: To characterize pig-tailed macaque genetics by single nucleotide polymorphism (SNP) genetic marker mapping throughout the pig-tailed macaque genome. Research Aim 3: To establish the whole genome of the pigtailed macaque using long-read sequencing technology and de novo assembly, thereby enhancing the research value of this macaque species by enabling functional genomic approaches.

我们的动物资源赠款(P40 OD013117)的续期需要五年的资金来继续支持 约翰霍普金斯大学的无特定病原体(SPF)群的猪尾猕猴(Macaca Nomestrina) 该大学于2006年首次获得资助，并于2011年续签。它具有以下资源组件和 研究部分的目标是： A.资源构成部分的具体目标： 资源目标1：扩大目前SPF繁殖群体的规模，以增加雌性数量 用于繁殖和雄性，可出售给美国国立卫生研究院资助的约翰·霍普金斯大学和 在其他研究机构。 资源目标2：通过定期检测4种病毒的血清学和聚合酶链式反应来维持菌落的SPF状态 制剂：脑炎疱疹病毒1型(B病毒)、猿猴免疫缺陷病毒(SIV)、猿猴逆转录病毒(SRV) 和猿猴T淋巴营养病毒(STLV)。目前，殖民地的所有现任成员都已多次 这些药剂的检测结果为阴性。 资源目标3：维护所有动物的系谱和健康数据。此数据可在所有 动物在群体中，并将随着群体的增长而扩大。它是提供给我们动物的购买者的。 B.研究组成部分的具体目标：将现有的辫尾猕猴遗传数据扩大到a) 提高对尾辫猕猴的研究效用和b)协助对短尾猴的遗传和疾病管理 殖民地。这将通过三个研究目标来实现。 研究目标1：通过MHC I类和MHC类对猪尾猕猴免疫遗传学进行详细表征 II基因分型。 研究目的2：利用单核苷酸多态性(SNP)研究猪尾短尾猴的遗传特征 整个猪尾猕猴基因组的标记图谱。 研究目的3：利用长阅读测序技术建立辫尾猕猴的全基因组 技术和从头组装，从而通过使这一猕猴物种能够 功能基因组学方法。