Development of an spf Macaca nemestrina breeding colony

我们的动物资源赠款（P40 OD 013117）的更新要求五年的资金继续支持 在约翰霍普金斯的无特定病原体（SPF）的猪尾猕猴（Macaca nemestrina）群体中， 该大学于2006年首次获得资助，并于2011年更新。它有以下资源组成部分， 研究部分的目标是： A.资源组成部分的具体目标： 资源目标1：扩大现有SPF繁殖群体的规模，以增加雌性的数量 用于繁殖和雄性供销售给NIH资助的约翰霍普金斯大学和 在其他研究机构。 资源目标2：通过定期血清学和PCR检测4种病毒，维持殖民地的SPF状态 病原体：猕猴疱疹病毒1型（B病毒）、猿猴免疫缺陷病毒（SIV）、猿猴逆转录病毒（SRV） 和猿猴T淋巴细胞病毒（STLV）。目前，殖民地的所有现任成员都一再表示， 对这些病原体检测呈阴性 资源目标3：维护所有动物的系谱和健康数据。这些数据可在所有 动物在殖民地，并将扩大作为殖民地增长。它提供给我们的动物购买者。 B。研究组成部分的具体目标：将猪尾猕猴的遗传数据扩展到a） 增加猪尾猕猴的研究效用，和B）协助猪尾猕猴的遗传和疾病管理， 殖民地这将通过三个研究目标来实现。 研究目的1：通过MHC-I类和MHC-II类来详细描述猪尾猕猴的免疫遗传学特征 II基因分型。 研究目的2：利用单核苷酸多态性（SNP）遗传学方法研究猪尾猕猴的遗传学特征 标记映射在猪尾猕猴基因组中。 研究目的3：利用长片段测序技术建立猪尾猕猴全基因组 技术和从头组装，从而通过使 功能基因组学方法。

This renewal of our animal resource grant (P40 OD013117) requests five years of funding to continue support for a Specific Pathogen Free (SPF) colony of pig-tailed macaques (Macaca nemestrina) at the Johns Hopkins University that was first funded in 2006 and renewed in 2011. It has the following resource component and research component aims: A. Resource Component Specific Aims: Resource Aim 1: To expand the size of the current SPF breeding colony to increase the numbers of females for breeding and males available for sale to NIH funded investigators both within Johns Hopkins University and at other research institutions. Resource Aim 2: To maintain the SPF status of the colony by regular serologic and PCR testing for 4 viral agents: Cercopithecine herpesvirus 1 (B virus), Simian Immunodeficiency Virus (SIV), Simian Retrovirus (SRV) and Simian T-lymphotrophic Virus (STLV). At present all of the current members of the colony have repeatedly tested negative for these agents. Resource Aim 3: To maintain pedigree and health data on all animals. This data is available on all of the animals in the colony and will be expanded as the colony grows. It is provided to purchasers of our animals. B. Research Component Specific Aims: To expand the genetic data available for pigtailed macaques to a) increase the research utility of pigtailed macaques and b) assist in genetic and disease management of the colony. This will be done through three research aims. Research Aim 1: To characterize pig-tailed macaque immunogenetics in detail via MHC class I and MHC class II genotyping. Research Aim 2: To characterize pig-tailed macaque genetics by single nucleotide polymorphism (SNP) genetic marker mapping throughout the pig-tailed macaque genome. Research Aim 3: To establish the whole genome of the pigtailed macaque using long-read sequencing technology and de novo assembly, thereby enhancing the research value of this macaque species by enabling functional genomic approaches.