Nanoassay development

荧光光谱学本质上是一种非常敏感的技术;它已经形成了 基于大多数非放射性真实的时间测定，如PCR。 我们的实验室与 前研究员（现在在生物技术行业）开发PCR的替代品，如“CataCleave” 探针的SNP，导致以前的出版物。 我们继续研究DNA组分与多层金属纳米颗粒的物理和适当耦合 更快的PCR分析和FCS的使用（见MPM报告），以量化非常紧密的 亚微升液滴中的蛋白质-蛋白质和蛋白质-DNA结合（分析物 以亚飞摩尔量存在）。 我们已经研究了 使用MPM-FCS，G-四链体和线性形式之间的DNA量相似 和时间分辨荧光工具，目标是开发非常灵敏的“G-quad” 和适体检测分析。我们已经开始将适体经验转化为BCC/黑色素瘤检测合作，尽管迄今为止整个动物的适体分布一直很困难。 我们已经将时间分辨荧光检测与平移迁移率（FCS）结合起来，以帮助识别“游离”和“结合”的特征。 我们也在设计用于DNA/RNA探测的STAQ探针（见纳米镜项目），类似于catacleave，计划只对紧密结合位点进行超分辨。 最近，我们也使用TCSPC来开始解开适体异质性问题。

Fluorescence Spectroscopy is inherently a very sensitive technique; it already forms the basis of most non-radioactive real time assays like PCR. Our lab has collaborated with former fellows (now in biotech industry) to develop alternatives to PCR like "CataCleave" probes for SNPs, leading to previous publication. We continue to study the photophysics and proper coupling of DNA components to multilayer metal nanoparticles for much faster PCR analysis and the use of FCS (see MPM report) to quantify very tight protein-protein and protein-DNA binding in sub-microliter drops (analytes are present in sub-femtomole amounts). We had examined the structural transitions of similar amounts of DNA between G-quadruplexed and linear forms, using both MPM-FCS and Time-Resolved Fluorescence tools, with the goal of developing very sensitive 'G-quad' and aptamer detection assays. We have begun to translate aptamer experience into BCC/melanoma detection collaboration, although whole-animal aptamer distribution has been difficult to date. We have numerically combined time-resolved fluorescence detection with translational mobility (FCS) to help identify "free" and "bound" signatures for assay. We are also designing STAQ probes (see nanoscopy project) for DNA/RNA probing, analagous to catacleave, in plans to only superresolve tight binding sites. Recently we have used TCSPC to begin untangling aptamer heterogeneity questions as well.