Developing nanoscale electrophysiology sensors for robust intracellular recording

开发纳米级电生理学传感器以实现强大的细胞内记录

• 批准号: 9423772
• 负责人: Bianxiao Cui
• 金额: $ 31.1万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-15 至 2021-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9423772
• 关键词: Action Potentials; Address; Affect; Amplifiers; Biomedical Research; Carbon Dioxide; Cardiac Myocytes; Cell membrane; Cell model; Cells; Communities; Coupling; Custom; Dialysis procedure; Electrodes; Electron Microscopy; Electrophysiology (science); Electroporation; Environmental Risk Factor; Generations; Geometry; Goals; Incubators; Individual; Measurement; Measures; Membrane; Membrane Potentials; Methods; Nanotechnology; Neurons; Nonlinear Dynamics; Outcome; Performance; Physiologic pulse; Physiological; Process; Resistance; Shapes; Signal Transduction; System; Techniques; Technology; Temperature; Time; Validation; Variant; Work; base; brain cell; design; electrical potential; extracellular; heart cell; human stem cells; improved; miniaturize; minimally invasive; nanoscale; new technology; patch clamp; programs; seal; sensor; solid state; tool

Project Summary / Abstract: Action potentials of electrogenic cells, such as neurons and cardiomyocytes, are crucial for their physiological functions. Neurons use action potential to transmit signals over long distances, and cardiomyocytes use action potentials to synchronize the contraction of millions of cells during each heartbeat. To understand these important physiological functions, one of the most important tactics is to accurately record the electrical potentials from cells. However, the current two major classes of electrophysiological methods, intracellular and extracellular recording, suffer severe limitations in their applications. Intracellular recording such as patch clamp suffers from extremely low throughput and toxic intracellular dialysis. Extracellular recording such as planar electrode array suffers from poor signal and lack of one-to-one cell-to-electrode coupling. In the last decade, much effort has been focused on developing new generation of electrophysiology tools to achieve high throughput intracellular recording. In particular, nanotechnology-based electrode sensors developed independently in several groups has shown great promise in achieving highly sensitive and high throughput intracellular recording. However, developing these nascent technologies into robust electrophysiological tools would require extensive studies for characterization, validation, and optimization. This proposal aims to develop the nanoelectrode technology into robust electrophysiological tools for biomedical research. When accomplished, this new technology will enable users to (a) perform sensitive, intracellular recording of action potentials from tens to hundreds of individual cells simultaneously; (b) achieve long-term, minimally-invasive recording of the cells for days to weeks; and (c) afford stable culture and recording of the hSC-CMs under optimal environmental conditions.

项目概要/摘要： 生电细胞（如神经元和心肌细胞）的动作电位对于它们的功能至关重要。 生理功能。神经元利用动作电位长距离传递信号，心肌细胞 利用动作电位来同步每一次心跳中数百万个细胞的收缩。了解 这些重要的生理功能，其中一个最重要的战术是准确地记录电 细胞的潜能。然而，目前主要有两类电生理方法，细胞内和 细胞外记录在它们的应用中受到严重的限制。细胞内记录，如膜片钳 具有极低的通量和毒性细胞内透析。细胞外记录，如平面 电极阵列遭受信号差和缺乏一对一的细胞-电极耦合。在过去的十年里， 许多努力都集中在开发新一代电生理学工具上， 通过细胞内记录。特别是，基于纳米技术的电极传感器开发 在实现高灵敏度和高通量方面显示出巨大的前景 细胞内记录然而，将这些新生技术发展成强大的电生理工具， 需要进行广泛的研究以进行表征、验证和优化。该提案旨在发展 将纳米电极技术转化为生物医学研究的强大电生理工具。一旦完成， 这项新技术将使用户能够（a）执行敏感的细胞内动作电位记录， （B）实现对细胞的长期、微创记录， 细胞数天至数周;和（c）在最佳环境条件下提供hSC-CM的稳定培养和记录 条件