Innovative Technology and Instrumentation (ITI) Core

项目摘要 NEI赞助的研究人员在斯坦眼科研究所（SEI）的研究呼吁， 开发和修改创新的光谱，电生理，光学和 机械系统。为了满足这一需求，一种最先进的计算机数控 (CNC)在SEI建立了制造和工程设施。该设施使 新设备和仪器的构建，引导SEI研究人员进入未探索的领域， 令人兴奋的研究领域。比如哈贝尔博士要求设计和建造 电子顺磁共振（EPR）光谱仪在高静水压下的操作 压力揭示蛋白质在视觉信号转导中的功能动力学。霍维茨医生的 研究需要为远距离和近距离采样定制的圆二色性光谱仪 高压下透镜蛋白的UV CD。萨姆帕斯博士需要定制荧光 用于记录视网膜细胞的单光子反应的显微镜。格拉斯哥博士的研究 需要定制的荧光计以及单分子共聚焦和超分辨率 用于测量泪液层中蛋白质动力学的寿命荧光的显微镜 电影德默尔博士的研究需要定制的控制系统来跟踪眼睛 运动的患者研究斜视和定制的机械设备， 测量眼外肌张力和弹性。对于这些示例中的每一个， 仪器不是商业上可获得的，而是重新设计和制造的，或者 对现有设备进行了大规模改造。这一部分对PI的研究至关重要， 为CNC加工培训的工程人员提供支持。此外该 组件将提供仪器，以支持常见的生物化学和分子生物学 满足SEI实验室的需求，维护和维修现有的高科技仪器， 核心实验室

Project Summary The NEI sponsored research of investigators at the Stein Eye Institute (SEI) calls for the development and modification of innovative spectroscopic, electrophysiological, optical and mechanical systems. To support this need, a state-of-the-art computer numerical controlled (CNC) fabrication and engineering facility has been established at the SEI. This facility enables the construction of novel devices and instruments that lead SEI investigators into unexplored and exciting areas of research. For example, Dr. Hubbell requires the design and construction of Electron Paramagnetic Resonance (EPR) spectrometers for operation at high hydrostatic pressure to reveal functional dynamics of proteins in visual signal transduction. Dr. Horwitz's research demands circular dichroism spectrometers customized for sampling both far and near UV CD of lens proteins at high pressure. Dr. Sampath requires customized fluorescent microscopes built for recording single photon responses of retinal cells. Dr. Glasgow's research demands customized fluorometers as well as single molecule confocal and super-resolution microscopes for measuring lifetime fluorescence of protein dynamics in the layers of the tear film. Dr. Demer's research necessitates customized control systems for tracking eye movements in patients to study strabismus and well as customized mechanical devices for measuring extraocular muscle tension and elasticity. For each of these examples, instrumentation is either not available commercially and is designed and built de novo, or existing equipment is extensively modified. This component is essential to the PI's research by providing support for engineering personnel trained in CNC fabrication. In addition, the component will provide instrumentation to support common biochemistry and molecular biology needs of SEI laboratories and maintain and repair existing high technology instrumentation in the core laboratory.