SHARED RESOURCES CORE

共享资源核心

• 批准号: 9281859
• 负责人: MARK E LAUER
• 金额: $ 25.58万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9281859
• 关键词: Binding; Blood Vessels; Cell Culture Techniques; Clinic; Clinical; Data; Diabetes Mellitus; Electronic Mail; Future; Gene Expression; Glycosaminoglycans; Goals; Hyaluronan; Ice; Immunohistochemistry; Individual; Inflammatory Bowel Diseases; Instruction; Modification; Notification; Pathology; Patients; Postdoctoral Fellow; Proteoglycan; Public Health; Pulmonary Hypertension; Research; Research Project Grants; Resource Sharing; Role; Sampling; Services; Smooth Muscle; Standardization; Techniques; Time; Unspecified or Sulfate Ion Sulfates; Vascular Diseases; Work; analytical tool; hands on instruction; meetings; programs; skill acquisition; skills; sulfation

Instmctions): (Core B, Lauer) The Shared Resource Core is one of the three cores required by the Cleveland Clinic Program of Excellence in Glycoscience (CC-PEG). The primary objectives of this Core are: 1) to accelerate the pace of the individual research projects by providing them with the glycoscience skills and facilities necessary for glycosaminoglycan analysis, and 2) to provide post-doctoral fellows supported by this program with hands-on instruction in glycosaminoglycan analytical techniques as part of a close partnership with the Glycoscience Skills Development Core. The Shared Resource Core will provide services and instruction in a variety of analytical techniques, involving glycosaminoglycans: (a) extraction and purification, (b) mass, size and sulfation profiling, (c) binding partners and modifications, (d) immunohistochemistry and gene expression, and (e) smooth muscle/endothellal cell culture. These services and instructions will insure timeliness, efficiency and uniformity for all the projects in achieving their aims that require extensive analyses of glycosaminoglycans/proteoglycans, thereby facilitating meeting the overall goals of the CC-PEG to determine the roles of hyaluronan matrices in vascular pathologies. RELEVANCE (See instructions): The Shared Resource Core will contribute to public health by providing the Individual research projects with the specialized analytical tools required for glycosaminoglycan/proteoglycan analyses. These analyses will involve clinical samples from patients with vascular disease involving diabetes, pulmonary hypertension and inflammatory bowel disease.

直觉）： (Core B、劳尔）共享资源核心是克利夫兰诊所要求的三个核心之一 糖科学卓越计划（CC-PEG）。本核心的主要目标是：（1）加快 通过为他们提供糖科学技能和设施， 必要的糖胺聚糖分析，和2）提供博士后研究员支持这个计划 与糖胺聚糖分析技术的实践指导，作为与 Glycoscience Skills Development Core.共享资源核心将提供服务和指导， 涉及糖胺聚糖的各种分析技术：（a）提取和纯化，（B）质量、大小 和硫酸化谱，（c）结合伴侣和修饰，（d）免疫组织化学和基因 表达，和（e）平滑肌/内皮细胞培养。这些服务和说明将确保 所有项目在实现需要广泛分析的目标方面的及时性、效率和一致性 的糖胺聚糖/蛋白聚糖，从而有助于满足CC-PEG的总体目标， 确定透明质酸基质在血管病理中的作用。 相关性（参见说明）： 共享资源核心将通过为个人研究项目提供 糖胺聚糖/蛋白聚糖分析所需的专用分析工具。这些分析将 涉及来自患有血管疾病的患者的临床样品，所述血管疾病涉及糖尿病、肺动脉高压和 炎症性肠病