SHARED RESOURCES CORE

实例)： (核心B，劳尔)共享资源核心是克利夫兰诊所需要的三个核心之一 糖科学卓越计划(CC-PEG)。此核心的主要目标是：1)加速 通过向他们提供糖科学技能和设施来加快个人研究项目的步伐 糖胺多糖分析所必需的，以及2)提供该计划支持的博士后研究员 在糖胺多聚糖分析技术方面进行实践指导，作为与 血糖科学技能开发核心。共享资源核心将提供服务和指令 涉及糖胺多聚糖的各种分析技术：(A)提取和纯化；(B)质量、大小 和硫酸盐分析，(C)结合伙伴和修饰，(D)免疫组化和基因 (E)平滑肌/内皮细胞培养。这些服务和说明将确保 所有项目在实现其需要广泛分析的目标方面的及时性、效率和一致性 糖胺多聚糖/蛋白多聚糖，从而促进实现CC-PEG的总体目标 确定透明质酸基质在血管病理中的作用。 相关性(请参阅说明)： 共享资源核心将为公共卫生做出贡献，为个人研究项目提供 糖胺多聚糖/蛋白多聚糖分析所需的专门分析工具。这些分析将 涉及糖尿病、肺动脉高压和高血压的血管疾病患者的临床样本 炎症性肠病。

Instmctions): (Core B, Lauer) The Shared Resource Core is one of the three cores required by the Cleveland Clinic Program of Excellence in Glycoscience (CC-PEG). The primary objectives of this Core are: 1) to accelerate the pace of the individual research projects by providing them with the glycoscience skills and facilities necessary for glycosaminoglycan analysis, and 2) to provide post-doctoral fellows supported by this program with hands-on instruction in glycosaminoglycan analytical techniques as part of a close partnership with the Glycoscience Skills Development Core. The Shared Resource Core will provide services and instruction in a variety of analytical techniques, involving glycosaminoglycans: (a) extraction and purification, (b) mass, size and sulfation profiling, (c) binding partners and modifications, (d) immunohistochemistry and gene expression, and (e) smooth muscle/endothellal cell culture. These services and instructions will insure timeliness, efficiency and uniformity for all the projects in achieving their aims that require extensive analyses of glycosaminoglycans/proteoglycans, thereby facilitating meeting the overall goals of the CC-PEG to determine the roles of hyaluronan matrices in vascular pathologies. RELEVANCE (See instructions): The Shared Resource Core will contribute to public health by providing the Individual research projects with the specialized analytical tools required for glycosaminoglycan/proteoglycan analyses. These analyses will involve clinical samples from patients with vascular disease involving diabetes, pulmonary hypertension and inflammatory bowel disease.