Georgia Comprehensive Metabolomics and Proteomics Unit for MoTrPAC

佐治亚州 MoTrPAC 综合代谢组学和蛋白质组学单位

• 批准号: 9246760
• 负责人: Dean Paul Jones
• 金额: $ 8.45万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-12-08 至 2022-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9246760
• 关键词: 3-Dimensional; Adipocytes; Affect; Aging; Animal Experimentation; Animals; Automobile Driving; Big Data; Bioinformatics; Biological Assay; Chemicals; Clinical; Clinical Research; Clinical Sciences; Collaborations; Communication; Complement; Complex; Custom; Data; Data Coordinating Center; Development; Dimensions; Disease; Educational workshop; Elements; Environment; Exercise; Forensic Medicine; Goals; Health; Health Benefit; Immunoassay; Institutes; Isotopes; Label; Laboratories; Leadership; Link; Lipids; Liquid Chromatography; Machine Learning; Malignant Neoplasms; Maps; Mass Spectrum Analysis; Measurement; Measures; Mediating; Methods; Modality; Molecular; Molecular Structure; Molecular Target; Monitor; Muscle; NMR Spectroscopy; Neurodegenerative Disorders; Nuclear; Oral; Oxidation-Reduction; Oxidative Stress; Particle Size; Peptide Mapping; Phosphotransferases; Physical activity; Plasma; Post-Translational Protein Processing; Procedures; Process; Proteins; Proteome; Proteomics; Protocols documentation; Publications; Reaction; Records; Research; Research Personnel; Resolution; Sampling; Scientist; Signal Transduction; Site; System; Systems Biology; Technology; Time; Training; Transducers; Translational Research; United States National Institutes of Health; Universities; Validation; advanced system; analytical method; chemoproteomics; cytokine; data resource; design; differential expression; innovation; instrumentation; ion mobility; lectures; member; metabolomics; molecular imaging; novel; oxidized lipid; phenotypic biomarker; response; small molecule; tandem mass spectrometry; tool; ultra high resolution

PROJECT SUMMARY The Georgia Comprehensive Metabolomics and Proteomics Unit for MoTrPAC (CMP-GA) is a cross-cutting team with advanced high-throughput chemical analytics and big data capabilities to develop a comprehensive map of molecular transducers of physical activity. The investigative team excels in ultrasensitive, precise and spatially resolved analyses of small molecules, lipids, and proteins. The team members have strong academic records as innovative, independent scientists, core laboratory directors and effective collaborators in complex research initiatives. Instrumentation includes state-of-the-art ultra-high resolution accurate mass high-field Orbitrap tandem mass spectrometry (MS) and ultraperformance liquid chromatography (UPLC); three- dimensional (3-D) molecular imaging via high throughput multi-modal MS at 5 m resolution; unique ion mobility-mass spectrometry machine-learning approaches for chemical identifications; and other such as GC- Orbitrap, 1D and 2D high field (800 MHz) NMR spectroscopy, ICP-MS, immunoassays, chemical and enzymatic assays, etc. The analytical laboratories are integrated through the NIH-supported Atlanta Clinical and Translational Sciences Institute, and operate within the outstanding research environment of Emory University and the Georgia Institute of Technology (GA Tech). CMP-GA has six elements organized to provide 1) leadership in the design and implementation of MoTrPAC goals; 2) bioinformatics, computational support and data delivery to the MoTrPAC Data Coordinating Center; 3) global, targeted and spatially resolved metabolomics; 4) metabolite forensics for unequivocal chemical identification of novel molecular transducers; 5) innovative proteomic and chemoproteomic technologies to define transducers within the redox proteome, ubiquitinome, acetylome, kinome and nuclear proteome; and 6) identification and quantification of lipid transducers. Through the successful completion of these aims and collaboration with the MoTrPAC consortium, investigators of CMP-GA will deliver a publically-available data resource and molecular transducer map that will enhance and accelerate mechanistic research on diseases and conditions affected by physical activity.

项目概要 佐治亚州 MoTrPAC 综合代谢组学和蛋白质组学单位 (CMP-GA) 是一个跨领域的机构 团队拥有先进的高通量化学分析和大数据能力，开发全面的 身体活动的分子传感器图。调查团队擅长超灵敏、精确和 小分子、脂质和蛋白质的空间分辨分析。团队成员学术实力雄厚 记录作为复杂的创新、独立科学家、核心实验室主任和有效合作者 研究举措。仪器包括最先进的超高分辨率精确质量高场 Orbitrap串联质谱（MS）和超高效液相色谱（UPLC）；三- 通过高通量多模态 MS 以 5 µm 分辨率进行三维 (3-D) 分子成像；独特离子 用于化学鉴定的迁移率质谱机器学习方法；以及其他诸如 GC- Orbitrap、一维和二维高场 (800 MHz) NMR 波谱、ICP-MS、免疫测定、化学和 酶分析等。分析实验室通过 NIH 支持的亚特兰大临床中心进行整合 和转化科学研究所，并在埃默里大学出色的研究环境中运作 大学和佐治亚理工学院（GA Tech）。 CMP-GA 有六个要素组织来提供 1) 领导 MoTrPAC 目标的设计和实施； 2）生物信息学、计算支持 并将数据传送至 MoTrPAC 数据协调中心； 3) 全局性、针对性和空间分辨率 代谢组学； 4）代谢物取证，用于新型分子传感器的明确化学鉴定； 5) 创新的蛋白质组学和化学蛋白质组学技术，用于定义氧化还原蛋白质组内的传感器， 泛素组、乙酰组、激酶组和核蛋白质组； 6) 脂质的鉴定和定量 换能器。通过成功实现这些目标以及与 MoTrPAC 的合作 CMP-GA 联盟的研究人员将提供公开可用的数据资源和分子传感器 该地图将加强和加速对受物理影响的疾病和状况的机制研究 活动。