Imaging Core

成像核心

• 批准号: 9488981
• 负责人: Zhenwu Zhuang
• 金额: $ 14.65万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9488981
• 关键词: Adult; Angiography; Archives; Blood Flow Velocity; Blood Vessels; Blood flow; Calibration; Catheterization; Clinic; Contrast Media; Data; Embryo; Equipment; Evaluation; Gene Targeting; Hindlimb; Human; Image; Imaging Techniques; Imaging technology; Injections; Ischemia; Measurement; Methodology; Molecular; Monitor; Mus; Operative Surgical Procedures; Organelles; Patients; Perfusion; Permeability; Phenotype; Physiologic Monitoring; Play; Preparation; Probability; Procedures; Reproducibility; Research; Research Personnel; Resolution; Role; Services; Specialist; Specimen; Stains; Standardization; Structure; System; Techniques; Time; Tissue imaging; Translating; Translations; X-Ray Computed Tomography; blood flow measurement; clinical practice; ex vivo imaging; experimental study; imaging study; in vivo; in vivo imaging; microCT; novel; programs; reconstruction; success; tissue preparation

Abstract/Summary: Core B will continue to serve the role it plays in our present PPG, while introducing two novel CT imaging components for phenotyping embryonic arteriogenesis in mice and assessing permeability in the setting of hindlimb ischemia in adult mice. All three projects extensively utilize Doppler flow velocity system for blood flow measurements and high-resolution microCT imaging for arteriogenesis phenotyping, with molecular, cellular and sub-organelle studies. For embryonic phenotyping, the Core will perform primary services necessary for the desired imaging procedures such as catheterization and en bloc staining. The Core will execute high-resolution microCT image acquisition, reconstruction, and analysis. These standardized efforts will result in optimal image acquisition and processing, reduce variability and maximize data reliability, while facilitating the translation of ideas from the bench to mice, and ultimately to humans. Core B procedures will be performed by two research specialists under the direction of Dr. Zhenwu Zhuang.

摘要/总结：核心B将继续在我们目前的PPG中发挥作用，同时引入两个 用于对小鼠胚胎动脉发生进行表型分型和评估血管通透性的新型CT成像组件 成年小鼠后肢缺血的设置。这三个项目都广泛利用多普勒流速系统 用于血流测量和动脉生成表型的高分辨率microCT成像， 分子、细胞和亚细胞器研究。对于胚胎表型，核心将进行主要的 所需成像程序所需的服务，如导管插入术和整体染色。核心 将执行高分辨率microCT图像采集、重建和分析。这些标准化 这些努力将导致最佳的图像采集和处理，减少变异性并最大限度地提高数据可靠性， 同时促进了想法从实验室到老鼠，最终到人类的转化。核心B程序 将由两位研究专家在庄振武博士的指导下进行。