Cell_Tissue_Microscopy_Core

核心D细胞和组织显微镜核心 Jyoti Jaiswal博士 核心总监 遗传医学研究中心和神经科学研究中心研究员， 儿童国家卫生系统 Anastas Popatiloff，医学博士，博士 核心联席董事 GW纳米制造和成像中心首席科学家兼主任 乔治华盛顿大学医学与健康科学学院 缅因州希瓦普拉萨德·布瓦南德兰 核心经理 职员科学家二级 遗传医学研究中心和神经科学研究中心 儿童国家卫生系统 克里斯汀·布兰特纳，博士 电子显微镜高级研究科学家 GW纳米制造和成像中心 乔治华盛顿大学医学与健康科学学院 摘要 细胞和组织显微镜核心(CTMC)的目标是提供对最先进的细胞和组织的访问 向DC-IDDRC调查人员提供成像技术，以支持、促进和加强针对IDD的工作 对细胞、组织和动物进行显微镜检查的研究。通过这种支持，CTMC 使DC地区的IDD研究人员能够重点追踪和调查各种儿科疾病 关于神经发育障碍、胎儿和新生儿脑损伤以及遗传性疾病。CTMC已经 传统上提供光学显微镜、电子显微镜和共焦显微镜服务。然而，由于 为了满足DC-IDD研究人员对增强成像分辨率的日益增长的需求，Core获得了新的 允许扩展其服务以包括先进的电子显微镜(EM)技术的仪器， 多光子和超分辨率成像能力。这些进展将使成像从纳米级到 整个组织和胚胎鳞片。这一增长得到了Core的显著扩展的补充 基础设施，允许处理大型数据集，并对大型结构和功能进行分析 数据集。CORE的进一步发展将显著增强IDD研究人员的能力 结合光遗传学和电生理学进行体内和体外功能成像。支持 在CTMC的这一增长中，已经聘请了两名新的核心经理，他们将他们的专业知识带到了体内 功能成像、EM及相关光和EM成像。这些人和其他敬业的核心工作人员 都是实践研究人员，不仅通过指定的核心活动提供他们的特定专业知识，而且 也通过协作项目。核心的这些能力通过合作进一步得到补充 科睿与多家显微镜和成像技术公司联手打造。此外，核心 与其他DC-IDDRC核心的接口，包括神经行为评估、基因组学和蛋白质组学， 以及人类和动物成像核心。我们将利用CTMC工作人员提供的专业知识和服务， 利用与此核心有关联的用户的参与，并与显微镜建立合作伙伴关系 公司，以提供以下服务：1)培训基于显微成像的研究人员 方法，2)帮助研究人员正确选择和使用光和EM工具来检查细胞 和组织，3)帮助开发和实施量化分析数据的方法 每个项目，4)保持高质量的数据采集、分析和发布，以及5)培训和 通过以下途径教育下一代碘缺乏病研究人员应用基于显微镜的技术 实际操作培训、特定技术教育和研讨会。 好了！

CORE D – CELL AND TISSUE MICROSCOPY CORE Jyoti Jaiswal, PhD Core Director Investigator, Center for Genetic Medicine Research & Center for Neuroscience Research, Children's National Health System Anastas Popratiloff, MD, PhD Core Co-Director Lead Scientist and Director, GW Nanofabrication and Imaging Center The George Washington University School of Medicine and Health Sciences Shivaprasad Bhuvanendran, ME Core Manager Staff Scientist ll Center for Genetic Medicine Research & Center for Neuroscience Research Children’s National Health System Christine Brantner, PhD Senior Research Scientist, Electron Microscopy GW Nanofabrication and Imaging Center George Washington University School of Medicine and Health Sciences Abstract The goal of the Cell and Tissue Microscopy Core (CTMC) is to provide access to state-of-the-art cell and tissue imaging technologies to DC-IDDRC investigators, in order to support, facilitate and enhance IDD-targeted research that involves microscopic examination of cells, tissues and animals. Through this support, the CTMC enables IDD researchers in the DC region to pursue and investigate a variety of pediatric diseases with a focus on neurodevelopmental disorders, fetal and neonatal brain injury, and genetic diseases. The CTMC has traditionally provided light microscopy, electron microscopy and confocal microscopy services. However, due to the growing need of DC-IDD researchers for enhanced imaging resolution, the Core has acquired new instruments that allow expansion of its services to include advanced electron microscopy (EM) technologies, Multiphoton, and super resolution imaging abilities. These developments will enable imaging from nanoscale to whole tissue and embryo scale. This growth has been supplemented by the significant expansion in Core’s infrastructure, which allow to handle large datasets, and carry out analysis of large structural and functional data sets. Additional developments in the Core will significantly enhance the ability of IDD researchers to perform in vivo and ex vivo functional imaging with combined optogenetics and electrophysiology. To support this growth in the CTMC, two new Core managers have been hired, who bring their expertise in in vivo functional imaging, EM, and correlative light and EM imaging. These and other dedicated Core staff members are all practicing researchers and offer their specific expertise not only through designated Core activities, but also through collaborative projects. These abilities of the Core are further supplemented by the collaborations forged between the Core and multiple microscopy and imaging technology companies. Additionally, the Core interfaces with other DC-IDDRC Cores, including the Neurobehavioral Evaluation, Genomics and Proteomics, and Human and Animal Imaging Cores. We will use the expertise and services offered by the CTMC staff, leverage on the involvement of users affiliated with this Core, and create partnerships with microscopy companies, in order to provide the following services: 1) training researchers in microscopy-based imaging approaches, 2) assisting researchers in proper choice and use of light and EM tools for examination of cells and tissues, 3) helping develop and implement approaches for quantitative analysis of the data obtained for each project, 4) maintaining high quality of data acquisition, analysis and publication, and 5) training and educating the next generation of IDD researchers in the application of microscopy-based technologies through hands on training, education on specific technologies, and workshops. !