Use of in vivo calcium imaging to study addiction.

摘要： 这个独立研究科学家奖的主要目标是让科琳博士的时间受到保护 McClung获得新的技术技能，这将补充她正在进行的成瘾研究计划 生物学这一奖项将使她成为熟练使用尖端，在体内钙成像 这项技术可以在几天到几周的时间内测量行为动物的神经元活动。她还将 学习如何处理和分析与此技术相关的大量数据。这将 包括与匹兹堡大学卡内基梅隆大学的杰出研究人员的动态合作 大学，斯坦福大学和山。西奈大学。这项技术将扩大和补充她 现有的研究，调查昼夜节律蛋白在奖励相关的大脑区域的作用， 药物成瘾的病理生理学具体来说，他们将允许她第一次测量神经元活动 在特定的神经元群体中，昼夜节律蛋白已经被操纵。这些 研究还将补充她目前的合作翻译项目，旨在了解 昼夜节律失调对青少年药物滥用风险的影响。这个奖项将 也为McClung博士提供了受保护的时间，以便与她在精神病学方面的临床同事进行互动， 在未来形成一个转化成瘾中心，专注于昼夜节律和睡眠的影响 干扰因此，该奖项将为未来的创新研究项目铺平道路，这将有助于我们更好地 了解和治疗成瘾症。

Abstract: The primary goal of this independent research scientist award is to allow protected time for Dr. Colleen McClung to acquire new technical skills which will complement her ongoing research program in addiction biology. This award will allow her to become proficient in the use of cutting edge, in vivo calcium imaging technology to measure neuronal activity in behaving animals over periods of days to weeks. She will also learn how to process and analyze the large amounts of data that are associated with this technology. This will involve dynamic collaborations with prominent researchers at the University of Pittsburgh, Carnegie Mellon University, Stanford University and Mt. Sinai University. This technology will expand and complement her existing studies which investigate the role of circadian proteins in reward-related brain regions in the pathophysiology of drug addiction. Specifically, they will allow her for the first time to measure neuronal activity over circadian time in specific neuronal populations in which circadian proteins have been manipulated. These studies will also complement her current collaborative translational projects which are aimed at understanding the influence of circadian rhythm misalignments in adolescents in the risk for substance abuse. This award will also allow protected time for Dr. McClung to interact with her clinical colleagues in psychiatry with the goal of forming a translational addiction center in the future focused on the influence of circadian rhythm and sleep disruptions. Thus this award will pave the way for future innovative research projects that will help us better understand and treat addiction disorders.