Multiscale Fabrication and Imaging Platform for Bioscience Applications

项目概要/摘要 需要新技术来研究树突状细胞中的纳米级细胞间通讯， 它与广泛的组织如骨骼、神经、心血管和 免疫系统拟议的工作将设计，建立和表征一个集成的多尺度制造 和成像平台，其能够使用生物相容的和通常 生物医学科学中使用的材料，并且能够在多个位置对感兴趣的生物进行非侵入性成像。 具有高分辨率和高速度。该项目将分为两个具体目标，两个模块 在每一个目标。Aim 1将专注于开发制造从厘米到厘米的设备的能力 以纳米分辨率使用常用的聚二甲基硅氧烷，合成聚乙二醇二丙烯酸酯 水凝胶和天然衍生明胶甲基丙烯酸酯水凝胶材料。在模块1中，混合加减 将开发基于超快激光器的工艺，以允许制造具有几个特征范围的器件 厘米至0.5微米，而在模块2中，STIMUTED发射耗尽（STED）启发光刻 将用于打印纳米结构，其特征范围从0.5微米到小于100纳米。 Aim 2将专注于在平台内集成超分辨率和3D断层成像功能。在 模块3，STED显微镜将被设计为实现亚100纳米的成像分辨率，而在模块 4、采用数字微镜器件-结构照明显微镜（DMD-SIM）实现高速 广角断层成像能力。我们将通过以下方式展示拟议平台的新功能： 开发一种多尺度流体装置，复制骨骼中发现的3D纳米级小管结构， 系统-这是目前技术无法完成的任务。如果成功，这项工作将使研究人员 提出关于广泛的细胞、组织、系统和疾病类型的新问题， 在没有这种技术的情况下进行了充分的研究。

Project Summary/Abstract New technology is needed to investigate nanoscale intercellular communication in dendritic cells, a process which shares commonality with a broad range of tissues such as the skeletal, nervous, cardiovascular, and immune systems. The proposed work will design, build and characterize an integrated multiscale fabrication and imaging platform capable of building multiscale devices and systems using biocompatible and commonly used materials in biomedical sciences, and capable of non-invasively imaging the biology-of-interest at multiple scales with high resolution and high speed. This project will be divided into two specific aims, with two modules in each aim. Aim 1 will focus on developing the capability of fabricating devices that could span from centimeter to nanometer resolution using commonly used polydimethylsiloxane, synthetic polyethylene glycol diacrylate hydrogel and naturally derived gelatin methacrylate hydrogel materials. In module 1, a hybrid additive-subtractive process based on ultrafast lasers will be developed to allow fabrication of devices with a feature range of several centimeters to 0.5micrometer, while in module 2, STimulated Emission Depletion (STED) inspired lithography will be used to print nanoscale structures with a feature range from 0.5micrometer to less than 100nanometers. Aim 2 will focus on integrating super resolution and 3D sectional imaging capabilities within the platform. In module 3, STED microscopy will be designed to achieve an imaging resolution of sub-100nm, while in module 4, Digital Micromirror Device-Structural Illumination Microscopy (DMD-SIM) will be used to achieve high-speed wide-angle sectional imaging capabilities. We will demonstrate the new capabilities of the proposed platform by developing a multiscale fluidic device that replicates the 3D nanoscale canaliculi structure found in the skeletal systems – a task not possible with current technology. If successful, the proposed work will enable researchers to ask new questions concerning a broad range of cells, tissues, systems and disease types that could not be studied adequately in the absence of such a technology.