Nanoparticle Synthesis and Characterization

共享资源核-纳米粒子合成与表征核(核A) 项目摘要/摘要 MC2TCN的愿景是推进、翻译和传播一套超小型(10 Nm)有机二氧化硅 混合纳米颗粒在肿瘤治疗中的应用。为了支持拟议的研究， 实现这一首要目标，共享资源核心(SRC)在纳米颗粒合成和 表征(核心-A)将提供临床级的颗粒合成和表征研究 颗粒尺寸小于10 nm的C‘点和C环。此核心的重点将是提供 分析颗粒大小和颗粒表面化学中的异质性的仪器和专业知识 使中心能够研究这些异质性如何调节药代动力学(PK)、清除量 轮廓和靶背景比，这是医学中纳米材料的一个关键问题，但通常具有挑战性 地址。这将通过以下方式实现：(1)添加高效液相色谱(HPL C)。 具有基于表面化学的颗粒分离的颗粒特定检测设备的系统，(2) 添加凝胶渗透色谱(GPC)系统，用于根据颗粒大小进行颗粒分离，(3) 获取用于光学实验的激光调谐到封装成染料的吸收特性 各种光学纳米粒子。其他需要合成和表征整个中心使用的设备 纳米粒子已经存在于康奈尔大学的空间中，那里将是核心的所在地，包括引擎盖空间，一个 手套箱、Zeta-sizer和动态光散射。该核心将为所有研究项目(RP1-4)提供服务。 中心。

SHARED RESOURCE CORE - NANOPARTICLE SYNTHESIS & CHARACTERIZATION CORE (CORE A) Project Abstract/Summary The vision of MC2TCN is to advance, translate, and disseminate a suite of ultrasmall (<10 nm) silica-organic hybrid nanoparticles for theranostics applications in oncology. In order to support the proposed research to achieve this overarching goal, the Shared Resources Core (SRC) on Nanoparticle Synthesis and Characterization (Core-A) will provide for particle synthesis and characterization investigations of clinical-grade C' dots and C rings with particle sizes below 10 nm. Particular focus of this Core will be to provide instrumentation and expertise in the analysis of heterogeneities in particle size and particle surface chemistry enabling studies in the Center on how these heterogeneities modulate pharmacokinetics (PK), clearance profiles, and target-to-background ratios, a key issue of nanomaterials in medicine but usually challenging to address. This will be accomplished by (1) addition of a high performance liquid chromatography (HPLC) system with particle specific detection equipment for particle separations based on surface chemistry, (2) addition of a gel permeation chromatography (GPC) system for particle separation based on particle size, (3) acquisition of lasers for optical experiments tuned to the absorption characteristics of dyes encapsulated into the various optical nanoparticles. Other equipment needed to synthesize and characterize Center-wide used nanoparticles already exist in the space at Cornell, where the Core will be located, and includes hood space, a glove box, a zeta-sizer, and dynamic light scattering. This core will serve all research projects (RP1-4) of the Center.