Nanoparticle Synthesis and Characterization

共享资源核心-纳米颗粒合成和表征核心（核心A） 项目摘要/摘要 MC 2 TCN的愿景是推进，翻译和传播一套超小（<10 nm）硅有机 用于肿瘤治疗诊断学应用的混合纳米颗粒。为了支持拟议的研究， 实现这一总体目标，纳米颗粒合成的共享资源核心（SRC）， 表征（核心-A）将提供临床级的颗粒合成和表征研究 粒径小于10 nm的C'点和C环。本核心的特别重点将是提供 粒度和颗粒表面化学不均匀性分析的仪器和专业知识 使中心能够研究这些异质性如何调节药代动力学（PK）、清除率 轮廓，目标与背景比，这是医学中纳米材料的关键问题，但通常具有挑战性， 地址.这将通过（1）添加高效液相色谱（HPLC） 具有用于基于表面化学的颗粒分离的颗粒特异性检测设备的系统，（2） 添加凝胶渗透色谱（GPC）系统，用于基于粒度的颗粒分离，（3） 获得用于光学实验的激光器，该激光器被调谐到封装在 各种光学纳米粒子。合成和表征中心范围内使用的 纳米粒子已经存在于康奈尔大学的空间中，核心将位于那里，包括罩空间， 手套箱，zeta-sizer，和动态光散射。该核心将服务于所有研究项目（RP 1 -4）， 中心

SHARED RESOURCE CORE - NANOPARTICLE SYNTHESIS & CHARACTERIZATION CORE (CORE A) Project Abstract/Summary The vision of MC2TCN is to advance, translate, and disseminate a suite of ultrasmall (<10 nm) silica-organic hybrid nanoparticles for theranostics applications in oncology. In order to support the proposed research to achieve this overarching goal, the Shared Resources Core (SRC) on Nanoparticle Synthesis and Characterization (Core-A) will provide for particle synthesis and characterization investigations of clinical-grade C' dots and C rings with particle sizes below 10 nm. Particular focus of this Core will be to provide instrumentation and expertise in the analysis of heterogeneities in particle size and particle surface chemistry enabling studies in the Center on how these heterogeneities modulate pharmacokinetics (PK), clearance profiles, and target-to-background ratios, a key issue of nanomaterials in medicine but usually challenging to address. This will be accomplished by (1) addition of a high performance liquid chromatography (HPLC) system with particle specific detection equipment for particle separations based on surface chemistry, (2) addition of a gel permeation chromatography (GPC) system for particle separation based on particle size, (3) acquisition of lasers for optical experiments tuned to the absorption characteristics of dyes encapsulated into the various optical nanoparticles. Other equipment needed to synthesize and characterize Center-wide used nanoparticles already exist in the space at Cornell, where the Core will be located, and includes hood space, a glove box, a zeta-sizer, and dynamic light scattering. This core will serve all research projects (RP1-4) of the Center.