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Microsatellite Sequencing to Enable Cancer Genotyping

微卫星测序可实现癌症基因分型

基本信息

批准号：
9756344
负责人：
Anna Schibel
金额：
$ 31.93万
依托单位：
ELECTRONIC BIOSCIENCES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2018
资助国家：
美国
起止时间：
2018-08-06 至 2021-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9756344
关键词：
Automobile Driving Back Basic Cancer Research Biological Sciences Biopsy Bladder Brain Breast Cancer cell line Cancerous Capillary Electrophoresis Cells Colon Colon Carcinoma Consensus Sequence Consumption DNA DNA Sequence Development Diagnostic Electronics Engineering Enzymes Evaluation Genome Genotype Goals Gold Government Head and neck structure Healthcare Hemolysin Human Human Genome Investigation Kidney Knowledge Length Liver Location Lung Malignant Neoplasms Measurement Methodology Methods Microsatellite Instability Microsatellite Repeats Motor Mutate Mutation Nucleotides Ovarian Patient Care Patients Persons Polymerase Population Predisposition Preparation Process Prostate Proteins Resolution Sampling Sensitivity and Specificity Single Nucleotide Polymorphism Skin Source Survival Rate Technology Third Generation Sequencing Thyroid Gland Time base cancer cell cancer type clinical application clinical diagnostics colon cancer cell line improved insertion/deletion mutation interest nanopore next generation sequencing novel strategies outcome forecast prevent prognostic prognostic assays programs single molecule synthetic construct

项目摘要

Project Summary Electronic BioSciences (EBS) will investigate and develop methodologies to accurately sequence microsatellite regions within the human genome to enable cancer genotyping. Microsatellites are simple/short repeats (1-10 nucleotides in length) that occur in tandem 5-50 times, and are among the most variable types of DNA sequence in the genome. Mutations to these microsatellite regions include expansion or contraction of the repeat number, single nucleotide polymorphisms (SNPs), and/or insertions or deletions (indels), which have been documented with cancer predisposition, cancer onset, and/or prognosis for many types of cancer, including colon, breast, bladder, brain, head and neck, skin, thyroid, kidney, prostate, lung, ovarian, and liver. To date, however, there is no technology presently available that is ideally suited for microsatellite sequencing/characterization, which has significantly limited the understanding of microsatellite mutations and the development of associated diagnostic/prognostic assays. During this two-year program, EBS will focus on developing the methodology to sequence and genotype microsatellites from colon cancer cell lines via direct, electronic, nanopore-based, single-molecule sequencing. The investigations performed during this program will enable new approaches to probe the human genome with unprecedented detail in order to relate and statistically characterize microsatellite sequences that signify a specific cancer type, predisposition, onset, and/or prognosis, directly improving basic cancer research and enabling eventual improved clinical diagnostics and/or prognostics technologies.

项目总结