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Controlled Protein Translocation in Nanopores for Sequencing Applications

用于测序应用的纳米孔中的受控蛋白质易位

基本信息

批准号：
10645979
负责人：
Anna Schibel
金额：
$ 39.35万
依托单位：
ELECTRONIC BIOSCIENCES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-08-11 至 2024-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10645979
关键词：
Amino Acids Back Biological Biological Markers ChIP-seq Charge Complex Computer software DNA Diameter Disease Electrolytes Electronics Elements Enzymes Evaluation Exclusion Feasibility Studies Gene Expression Goals Homo Individual Length Ligation Lipid Bilayers Magnetism Measurement Measures Methodology Methods Monitor Motor Nucleic acid sequencing Peptide Sequence Determination Peptides Phase Positioning Attribute Post-Translational Protein Processing Preparation Protein translocation Proteins Proteome Proteomics RNA Reader Reading Frames Reproducibility Resolution Sampling Scheme Secondary Protein Structure Small Business Innovation Research Grant Stretching System Systems Integration Techniques Technology Time Variant War biological research data acquisition detection method high reward high risk improved instrumentation nanopore novel particle polypeptide prevent programs protein folding prototype research clinical testing sequencing platform single cell proteins single molecule synthetic peptide tool voltage

项目摘要

Project Summary In the field of proteomics, the ultimate goal is to determine the sequence and abundance of every protein variant in a sample. To reach this goal, we need techniques capable of single-molecule, high-accuracy, de novo protein sequencing. Unfortunately, current proteomics technologies lack the sensitivity, dynamic range, throughput, scale, and accuracy needed to meet this goal. During this project, we will develop technological components and methods required to enable state-of-the-art protein sequencing. More specifically, we will develop methods for controllably translocating a protein through a nanopore reader such that the protein can be sequenced with single amino acid resolution. Nanopore-based sequencing provides a path to characterizing both high- and low- abundance proteins by sequencing proteins one molecule at a time, but with the high accuracy, sensitivity and throughput needed to cover the wide dynamic range in protein abundance in the proteome. During this project, we will develop and build a novel prototype sequencing system, fully assess and optimize the associated workflow/methodology for highly accurate, reproducible, and versatile protein/peptide characterization, and demonstrate initial sequencing for various proteins and peptides. At the conclusion of this project, we will have successfully demonstrated feasibility for a practical nanopore-based protein sequencing concept.

项目总结