Towards Viable Nanopore Sequencing by Slowing DNA Translocation

描述(申请人提供)：这个项目的目的是研究一种简单的方法来减缓DNA通过纳米孔的电泳性移位的速度。在这种移位过程中，由单个碱基产生的跨纳米孔的独特电流调制形成了DNA纳米孔测序的基础。纳米孔法需要非常简单的样品制备，可能不需要扩增步骤，并且可以用廉价的硬件和生化试剂获得长阅读序列。因此，纳米孔技术在努力实现快速、低成本测序方面具有很大的前景。然而，由于DNA自由移位的极快性质，目前的高带宽方法受到无法准确区分不同碱基的限制。这里提出的方法通过生物和电子创新保持了纳米孔测序的简单性。通过这项提议取得的进展可能会导致一种廉价和快速的台式DNA测序解决方案，这将对实现1000美元基因组的目标做出重大贡献。

DESCRIPTION (provided by applicant): The aim of this project is to investigate a simple method for slowing down the rate of electrophoretic translocation of DNA through a nanopore. Unique modulations of current across nanopores produced by individual bases during such translocation forms the basis of DNA nanopore sequencing. The nanopore method requires very simple sample preparation with potentially no amplification steps and can achieve long read sequences with inexpensive hardware and biochemical reagents. Hence, nanopore technology holds a great deal of promise in the effort to achieve rapid, low cost sequencing. However, current high bandwidth methods are limited by the inability to accurately distinguish various bases due to the extremely rapid nature of freely translocating DNA. Methods proposed here maintain the simplicity of nanopore sequencing through biological and electronic innovations. Progress made through this proposal will potentially lead to an inexpensive and rapid benchtop DNA sequencing solution that will contribute significantly towards the goal of the $1000 genome.