Towards Viable Nanopore Sequencing by Slowing DNA Translocation

描述（由申请人提供）：该项目的目的是研究一种简单的方法，用于减缓DNA通过纳米孔的电泳易位速率。在这种移位过程中，单个碱基产生的跨纳米孔电流的独特调节形成了DNA纳米孔测序的基础。纳米孔方法需要非常简单的样品制备，可能没有扩增步骤，并且可以用廉价的硬件和生化试剂实现长读取序列。因此，纳米孔技术在实现快速、低成本测序的努力中具有很大的前景。然而，目前的高带宽方法由于自由易位DNA的极其快速的性质而受到无法准确区分各种碱基的限制。本文提出的方法通过生物和电子创新保持了纳米孔测序的简单性。通过该提案取得的进展将可能导致廉价和快速的台式DNA测序解决方案，这将大大有助于实现1000美元基因组的目标。

DESCRIPTION (provided by applicant): The aim of this project is to investigate a simple method for slowing down the rate of electrophoretic translocation of DNA through a nanopore. Unique modulations of current across nanopores produced by individual bases during such translocation forms the basis of DNA nanopore sequencing. The nanopore method requires very simple sample preparation with potentially no amplification steps and can achieve long read sequences with inexpensive hardware and biochemical reagents. Hence, nanopore technology holds a great deal of promise in the effort to achieve rapid, low cost sequencing. However, current high bandwidth methods are limited by the inability to accurately distinguish various bases due to the extremely rapid nature of freely translocating DNA. Methods proposed here maintain the simplicity of nanopore sequencing through biological and electronic innovations. Progress made through this proposal will potentially lead to an inexpensive and rapid benchtop DNA sequencing solution that will contribute significantly towards the goal of the $1000 genome.