Towards Viable Nanopore Sequencing by Slowing DNA Translocation

通过减缓 DNA 易位实现可行的纳米孔测序

• 批准号: 8570021
• 负责人: Anna Schibel
• 金额: $ 25万
• 依托单位: ELECTRONIC BIOSCIENCES, INC.
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8570021
• 关键词: Address; Biochemical; Biological; Characteristics; DNA; DNA Binding; DNA Sequence; Development; Discrimination; Disease; Electronics; Engineering; Enzymes; Escherichia coli; Evaluation; Failure; Genetic; Genome; Goals; Government; Health; Hemolysin; Human; Individual; Lead; Lipids; Measurement; Medicine; Methods; Motor; Nature; Noise; Nucleotides; Performance; Persons; Phase; Polynucleotides; Preparation; Proteins; RNA; Reading; Reagent; Relative (related person); Residual state; Right-On; SS DNA BP; Sampling; Single-Stranded DNA; Site-Directed Mutagenesis; Solutions; Speed; Stretching; Structure; System; Techniques; Technology; Time; base; biological systems; cost; electrical measurement; genome sequencing; improved; innovation; insertion/deletion mutation; instrumentation; melting; nanopore; novel; pathogen; programs; public health relevance; silicon nitride; single molecule; solid state; success; tool

DESCRIPTION (provided by applicant): The aim of this project is to investigate a simple method for slowing down the rate of electrophoretic translocation of DNA through a nanopore. Unique modulations of current across nanopores produced by individual bases during such translocation forms the basis of DNA nanopore sequencing. The nanopore method requires very simple sample preparation with potentially no amplification steps and can achieve long read sequences with inexpensive hardware and biochemical reagents. Hence, nanopore technology holds a great deal of promise in the effort to achieve rapid, low cost sequencing. However, current high bandwidth methods are limited by the inability to accurately distinguish various bases due to the extremely rapid nature of freely translocating DNA. Methods proposed here maintain the simplicity of nanopore sequencing through biological and electronic innovations. Progress made through this proposal will potentially lead to an inexpensive and rapid benchtop DNA sequencing solution that will contribute significantly towards the goal of the $1000 genome.

描述（由申请人提供）：该项目的目的是研究一种通过纳米孔减慢DNA电泳易位速率的简单方法。在这种易位过程中，由单个碱基产生的电流通过纳米孔的独特调制形成了DNA纳米孔测序的基础。纳米孔方法需要非常简单的样品制备，可能没有扩增步骤，并且可以用廉价的硬件和生化试剂获得长读取序列。因此，纳米孔技术在实现快速、低成本测序方面具有很大的前景。然而，由于DNA自由易位的极快性质，目前的高带宽方法无法准确区分各种碱基，因此受到限制。本文提出的方法通过生物和电子创新保持了纳米孔测序的简单性。通过这一提议取得的进展将有可能导致一种廉价和快速的台式DNA测序解决方案，这将为实现1000美元基因组的目标做出重大贡献。