Achieving Single Nucleotide Resolution to Enable DNA Flossing Through Alpha-Hemolysin
实现单核苷酸分辨率,通过 α-溶血素实现 DNA 牙线清洁
基本信息
- 批准号:9171327
- 负责人:
- 金额:$ 22.5万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2016
- 资助国家:美国
- 起止时间:2016-09-16 至 2018-02-28
- 项目状态:已结题
- 来源:
- 关键词:AddressAffectAutomobile DrivingBackBase SequenceBindingBiochemicalBiological ProcessBiological SciencesComplexConsensusConsensus SequenceDNADNA SequenceDevelopmentElectronicsElectrostaticsElementsEnzymesEpigenetic ProcessEvaluationForensic MedicineFrequenciesFutureGeometryGovernmentHealthHemolysinIndividualInvestigationLabelLesionLogicMeasuresMedicalMethodsModificationMonitorMotionMotorNational Human Genome Research InstituteNoiseNucleotidesPatientsPersonsPhasePolymersPreparationProteinsReadingReagentReportingResolutionRisk AssessmentSS DNA BPSamplingSeriesSideSignal TransductionSingle-Stranded DNASmall Business Innovation Research GrantSpeedStretchingSystemTechniquesTechnologyTemperatureTimebasecostimprovedinsertion/deletion mutationinstrumentationnanoporenext generationnovelpreventprogramsscreeningsequencing platformsingle moleculevoltage
项目摘要
Project Summary
Enabling routine DNA sequencing has the potential to improve the understanding of biological processes and
relationships as well as aid in medicine, forensics, and environmental investigations. However, the potential
future benefits of sequencing require a reduction in cost and speed, and an improvement in instrumentation
accessibility relative to presently commercially available technology. Among the various next generation DNA
sequencing technologies being pursued, nanopore strand sequencing has emerged as a very promising
alternative. Strand sequencing has the advantages of being a single-molecule technique that is capable of
long (kilobases) reads, requires minimal sample preparation and no sample labeling, uses relatively
inexpensive hardware and biochemical reagents, is high-throughput for efficient sequence coverage, and
utilizes direct readout with the potential to read epigenetic modifications and lesions as well as sequence other
polymers. Thus, to address the current improvements needed to enable routine DNA sequencing through the
use of nanopores, EBS proposes to develop methods to achieve single-nucleotide resolution without the use of
processive enzymes by taking advantage of EBS’ proprietary electronics, αHL pores and novel DNA slowing
mechanisms. These advancements will enable the development of a low complexity (i.e. no PCR needed),
high accuracy (indels and mismatches are resolved) nanopore strand sequencing approach.
项目总结
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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Anna Schibel其他文献
Anna Schibel的其他文献
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- 资助金额:
$ 22.5万 - 项目类别:
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