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Achieving Single Nucleotide Resolution to Enable DNA Flossing Through Alpha-Hemolysin

实现单核苷酸分辨率，通过 α-溶血素实现 DNA 牙线清洁

基本信息

批准号：
9171327
负责人：
Anna Schibel
金额：
$ 22.5万
依托单位：
ELECTRONIC BIOSCIENCES, INC.
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-16 至 2018-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9171327
关键词：
Address Affect Automobile Driving Back Base Sequence Binding Biochemical Biological Process Biological Sciences Complex Consensus Consensus Sequence DNA DNA Sequence Development Electronics Electrostatics Elements Enzymes Epigenetic Process Evaluation Forensic Medicine Frequencies Future Geometry Government Health Hemolysin Individual Investigation Label Lesion Logic Measures Medical Methods Modification Monitor Motion Motor National Human Genome Research Institute Noise Nucleotides Patients Persons Phase Polymers Preparation Proteins Reading Reagent Reporting Resolution Risk Assessment SS DNA BP Sampling Series Side Signal Transduction Single-Stranded DNA Small Business Innovation Research Grant Speed Stretching System Techniques Technology Temperature Time base cost improved insertion/deletion mutation instrumentation nanopore next generation novel prevent programs screening sequencing platform single molecule voltage

项目摘要

Project Summary Enabling routine DNA sequencing has the potential to improve the understanding of biological processes and relationships as well as aid in medicine, forensics, and environmental investigations. However, the potential future benefits of sequencing require a reduction in cost and speed, and an improvement in instrumentation accessibility relative to presently commercially available technology. Among the various next generation DNA sequencing technologies being pursued, nanopore strand sequencing has emerged as a very promising alternative. Strand sequencing has the advantages of being a single-molecule technique that is capable of long (kilobases) reads, requires minimal sample preparation and no sample labeling, uses relatively inexpensive hardware and biochemical reagents, is high-throughput for efficient sequence coverage, and utilizes direct readout with the potential to read epigenetic modifications and lesions as well as sequence other polymers. Thus, to address the current improvements needed to enable routine DNA sequencing through the use of nanopores, EBS proposes to develop methods to achieve single-nucleotide resolution without the use of processive enzymes by taking advantage of EBS’ proprietary electronics, αHL pores and novel DNA slowing mechanisms. These advancements will enable the development of a low complexity (i.e. no PCR needed), high accuracy (indels and mismatches are resolved) nanopore strand sequencing approach.

项目总结