Effects of Developmental Alcohol Exposure on Astrocyte Ca2+ Signaling and Structural Synaptic Plasticity

摘要 怀孕期间接触酒精会导致胎儿酒精谱系障碍(FASD)，这是 与不同神经心理领域的认知障碍有关。患有FASD的儿童经常 表现出感觉运动功能障碍，包括运动协调和精细运动控制方面的缺陷。 尽管发育期酒精暴露已被证明会影响星形胶质细胞并导致损伤 在几个脑区的神经元可塑性中，对星形胶质细胞钙离子的变化知之甚少。 在完整的大脑中发生的信号和突触变化。在这里，我们将使用产前暴露小鼠 利用胎儿酒精谱障碍模型研究酒精对基础和运动技能的影响 学习诱导星形胶质细胞钙信号和结构突触可塑性。我们将在体内结合 在基础状态和学习状态下，用星形细胞钙离子成像对树突棘进行成像。这个 该提案的目标是确定发育期酒精暴露是否会导致 基础学习和运动技能学习诱导了星形细胞钙信号和结构突触的可塑性。

Abstract Exposure to alcohol during pregnancy produces fetal alcohol spectrum disorders (FASD) which is associated with cognitive impairments in various neuropsychological domains. Children with FASD often exhibit sensorimotor dysfunctions that include deficits in motor coordination and fine motor control. Although developmental alcohol exposure has been shown to affect astrocytes and cause impairments in neuronal plasticity in several brain regions, very little is known about alterations in astrocytic Ca2+ signaling and synaptic changes that occur in the intact brain. Here we will use prenatal exposure mouse model of fetal alcohol spectrum disorders to study alcohol-induced alterations in basal and motor-skill learning induced astrocytic Ca2+ signaling and structural synaptic plasticity. We will combine in vivo imaging of dendritic spines with astrocytic Ca2+ imaging under basal conditions and with learning. The goal of the proposal is to determine whether developmental alcohol exposure results in alterations in basal and motor-skill learning induced astrocytic Ca2+ signaling and structural synaptic plasticity.