Core-002: Structural and Compositional Assessment Core

密歇根州综合肌肉骨骼健康核心中心-核心C 项目摘要项目摘要 跨生理层次的组成、结构和结构，在器官、组织、 基质和纳米级，是连接基因表达和功能的关键中间性状 结果；然而，分析这些特征需要先进而昂贵的工具和 支持化学、工程和生物医学成像方面的专业知识。结构和 组成评估核心(核心C)将提供对设施和技能的访问 知识渊博的专家是量化所有组件、架构和结构所必需的 肌肉骨骼组织。核心C将包括微型计算机断层扫描(µCT)、纳米级 计算机断层扫描(Nano-CT)、体内微型计算机断层扫描(In-vivoµCT)和 它的主要成分是拉曼光谱。选择这些技术是因为它们的广泛应用 适用性和他们在密歇根大学的庞大用户基础。密歇根州的调查人员 率先使用微CT和拉曼光谱研究肌肉骨骼组织，并 在应用这些技术解决肌肉骨骼问题方面处于领先地位。核心C将利用 他们的专业知识，在智力和财政上支持当前的研究界， 对所有肌肉骨骼组织进行结构和成分分析。核心C也将 作为其使命的一部分，重点是扩大其提供的服务，以增加现有的机会 并扩大研究社区。提出了四个目标：核心将提供 在目标1和目标2中，获得关于设计实验的专门知识和指导 最先进的技术和专家，允许量化组织成分、结构和 在这些方法上的结构和培训。此外，在目标3中，核心将提供指导 对结果进行解释，并对今后的研究提出建议。最后，总体目标是 目标4是使研究界能够通过持续的技术开发和 教育。遗传和/或环境扰动往往会导致可测量的变化 具有机械和其他功能后果的结构和建筑。因此， 准确地量化这些特征对于促进对机制的理解至关重要。 基本肌肉骨骼生物学和健康，并制定和评估战略 预防和治疗疾病。

Michigan Integrative Musculoskeletal Health Core Center – Core C Project Summary Project Summary Composition, architecture, and structure across physiological hierarchies, at the organ, tissue, matrix and nanoscale-levels, are key intermediate traits that link gene expression with functional outcomes; however, analysis of these traits requires advanced and costly instruments and supporting expertise in chemistry, engineering, and biomedical imaging. The Structural and Compositional Assessment Core (Core C) will provide access to the facilities and skilled knowledgeable experts necessary to quantify composition, architecture, and structure of all musculoskeletal tissues. Core C will include micro-Computed Tomography (µCT), nano- Computed Tomography (nano-CT), in-vivo micro-Computed Tomography (in-vivo µCT) and Raman spectroscopy as its major components. These technologies were chosen for their broad applicability and their large user base at the University of Michigan. Investigators at Michigan pioneered the use of µCT and Raman spectroscopy to study musculoskeletal tissues and are leaders in applying these technologies to solve musculoskeletal problems. Core C will leverage their expertise to support the current Research Community, both intellectually and financially, in performing structural and compositional analyses of all musculoskeletal tissues. Core C will also focus, as part of its mission, on expanding its offerings to enhance the opportunities available and to expand the Research Community. Four Aims are proposed: the Core will provide expertise and guidance on the design of experiments in Aim 1, and in Aim 2, access to state-of- the-art technologies and experts to allow quantification of tissue composition, architecture, and structure and training in these in methods. Further, in Aim 3, the Core will provide guidance with interpretation of results and make recommendations for future studies. Lastly, the overall goal of Aim 4 is to enable the research community through ongoing technology development and education. Genetic and/or environmental perturbations often lead to measureable changes in structure and architecture that have mechanical and other functional consequences. Therefore, accurately quantifying these traits is critical to advancing understanding of the mechanisms underlying musculoskeletal biology and health and to develop and evaluate strategies for the prevention and treatment of disease.